Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the effective and rapid methods for the diagnosis of virus infections. It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. Some of the topics covered in the book are the fluorescence microscopy; nature of fluorescence; filter systems; transmitted light microscopy; interference filters for transmitted light microscopy; lamp centration and alignment of microscope; contrast-fluorescence condensers; photomicrography; and choice of microscope. The culture of viruses for production of antiserum is fully covered. The selection of cell lines or tissues for virus culture is discussed in detail. The text describes in depth the fluorescent antibody staining techniques. The preparation of specimens from respiratory infections is presented completely. A chapter is devoted to the respiratory syncytial virus. Another section focuses on the analysis influenza virus, paramyxoviruses, and picornaviruses. The book can provide useful information to doctors, virologists, students, and researchers.

Viral respiratory tract infections are important and common causes of morbidity and mortality worldwide. In the past two decades, several novel viral respiratory infections have emerged with epidemic potential that threaten global health security. This Monograph aims to provide an up-to-date and comprehensive overview of severe acute respiratory syndrome, Middle East respiratory syndrome and other viral respiratory infections, including seasonal influenza, avian influenza, respiratory syncytial virus and human rhinovirus, through six chapters written by authoritative experts from around the globe.

THE ESSENTIAL WORK IN TRAVEL MEDICINE -- NOW COMPLETELY UPDATED FOR 2018 As unprecedented numbers of travelers cross international borders each day, the need for up-to-date, practical information about the health challenges posed by travel has never been greater. For both international travelers and the health professionals who care for them, the CDC Yellow Book 2018: Health Information for International Travel is the definitive guide to staying safe and healthy anywhere in the world. The fully revised and updated 2018 edition codifies the U.S. government's most current health guidelines and information for international travelers, including pretravel vaccine recommendations, destination-specific health advice, and easy-to-reference maps, tables, and charts. The 2018 Yellow Book also addresses the needs of specific types of travelers, with dedicated sections on: · Precautions for pregnant travelers, immunocompromised travelers, and travelers with disabilities · Special considerations for newly arrived adoptees, immigrants, and refugees · Practical tips for last-minute or resource-limited travelers · Advice for air crews, humanitarian workers, missionaries, and others who provide care and support overseas Authored by a team of the world's most esteemed travel medicine experts, the Yellow Book is an essential resource for travelers -- and the clinicians overseeing their care -- at home and abroad.

The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.

The traditional approach to diagnosis of virus infectians by isolation of the causative virus is usually both slow and expensive. More recently, the emphasis has been on the direct detection of viruses or viral antigens in clinical specimens. This can be done using established techniques such as immunofluorescence or electron microscopy, or by newly developed biochemical methods. The purpose of this meeting was to review these and other developments in the laboratory diagnosis of virus infections. We would like to thank all those who contributed to the success of this meeting. In particular, we are grateful to the CEC for financial sponsorship, to Professor C. Dow, Director of the Veterinary Research Laboratories, for help in organising the meeting, and to Miss B. Hamilton for her excellent typing of the manuscripts. M S McNulty J B McFerran VII CONTENTS Page Preface V VIRAL DIAGNOSIS BY TIMMUNOFLUORESCENCE P. S. Gardner APPLICATIONS OF IMMUNOFLUORESCENCE IN VETERINARY VIRAL DIAGNOSIS M. S. McNulty and G. M. Allan 15 TAGGING OF VIRAL ANTIGENS USING IMMUNOPEROXIDASE . AND IMMUNOOOLD TEGINlQUES R. Ducatelle, F. Castryck and J. Hoorens 27 DETECTION OF BVD VIRUS IN VlRAEMIC CATTLE BY AN INDIRECT IMMUNOPEROXIDASE TEGINIQUE A. Meyling 37 VIRAL DIAGNOSIS BY ELECTRON MICROSCOPY J. D. Almeida 47 CAPTURE ASSAYS FOR THE DETECTION OF VIRUS-SPECIFIC IgM ANTIBODY R. S. Tedder 60 DETECTION . AND IDENTIFICATION OF PAPILLOMAVIRUSES IN BENIGN . AND MALIGNANT 'TIlmJRS OF CATTLE M. S. Campo 72 DETECTION OF VIRAL NUCLEIC ACIDS IN CELL CULTURES .

Advanced Biosensors for Virus Detection: Smart Diagnostics to Combat Against the SARS-CoV2 Pandemic covers the development of biosensor-based approaches for the diagnosis and prognosis of viral infections, specifically coronaviruses. The book discusses wide-ranging topics of available biosensor-based technologies and their application for early viral detection. Sections cover the emergence of SARS-CoV, MERS-CoV and SARS-CoV2, the global health response, the impact on affected populations, state-of-the art biomarkers, and risk factors. Specific focus is given to COVID-19, with coverage of genomic profiling, strain variation and the pathogenesis of SARS-CoV2. In addition, current therapeutics, nano-abled advancements and challenges in the detection of SARS-CoV2 and COVID-19 management are discussed, along with the role of nanomaterials in the development of biosensors and how biosensors can be scaled up for clinical applications and commercialization. - Deals with biosensors-based approaches that could be exploited to design and develop high throughput, rapid and cost-effective diagnostics technologies for the early detection of viral infections - Illustrates the development of multiplexed, miniaturized analytical systems for point-of-care applications - Provides information about fabrication protocols for various biosensor based diagnostic approaches that could be directly implemented to develop a novel biosensor - Includes the past, present and future status of biosensors, along with information about biosensors currently under clinical trials

To Catch a Virus Trace the evolution of diagnostic virology from yellow fever to COVID-19 Join expert storytellers John Booss, Marilyn J. August, and Marie Louise Landry in a journey through the history of viral epidemics and the detective work of those determined to identify the culprits and treat the infected. From the identification of the first virus in the late 1800s to the molecular techniques that enabled the rapid recognition of and vaccine development for the SARS-CoV-2 virus, viral diagnostic methods have progressed over the past century to become a formidable tool in human health care. This collection of gripping historical narratives covers a range of fascinating outbreaks and public health challenges, from yellow fever and smallpox to AIDS and COVID-19. This new edition chronicles the ongoing story of the COVID-19 pandemic, highlighting the people, the pathogen, and the progress in the diagnostic laboratory and clinical settings that has touched every aspect of global health. The many photographs and rich biographical sketches of key figures, diagrams of diagnostic procedures, micrographs of virus-infected cells, timelines, and a new glossary of key terms make To Catch a Virus compelling reading. This book serves as an excellent resource for courses in virology, immunology, microbiology, and public health. As the world struggles with the ongoing pandemic of SARS-CoV-2/COVID-19, To Catch a Virus is an insightful and superbly told story that chronicles the incredible metamorphosis of diagnostic virology and the technological advances that now make it possible to quickly and accurately detect and monitor the many disease-causing viruses that plague humankind. A stimulating, informative, and absorbing read that is highly recommended. —Richard L. Hodinka, PhD, Professor Emeritus, Perelman School of Medicine at the University of Pennsylvania; former Director, Clinical Virology Laboratory, Children’s Hospital of Philadelphia To Catch a Virus provides a beautifully written and compelling story of scientific discovery. It carefully traces the understanding of viral diseases from the turn of the twentieth century to the present. For general readers the authors provide timely and expert guidance to the extraordinary advances in diagnosis, surveillance, and therapeutics that constitute the silver lining in the otherwise somber years of COVID-19. For anyone wishing to understand the challenges confronting virologists and their accomplishments to date, this work is the place to start. —Frank M. Snowden, PhD, Andrew Downey Orrick Professor Emeritus of History, Yale University; former Chair, Program in History of Science and History of Medicine, Yale University

Molecular Detection of Animal Viral Pathogens presents expert summaries on state-of-the-art diagnostic approaches for major animal viral pathogens, with a particular emphasis on identification and differentiation at the molecular level. Written by specialists in related research areas, each chapter provides a concise overview of an individual virus

Herpes Simplex Virus is the fifth monograph to be published in the Bloomsbury Series of Clinical Science. It provides an authoritative review of the key issues related to this common clinical problem. The characteristics of the virus, its epidemiology and the diag nosis and management of the various forms of infection are all considered. Adrian Mindel is an international authority on this subject; he joined the Academic Department of Genito-urinary Medicine at the Middlesex Hospital in 1980 and has been actively involved in HSV research since that time. His major research interests include the epidemiology and treatment of genital herpes, the epidemi ology of neo-natal herpes and the many and varied features of HSV infections in immuno-compromised patients. The continuing aim of the Bloomsbury Series is to identify the growing areas of clinical research and relate these to current and future medical practice. In Herpes Simplex Virus such aspirations have been successfully achieved. London, May 1989 Jack Tinker Preface There has been considerable interest in herpes simplex viruses (HSV) over recent years. Amongst the many reasons for this are the introduction of safe and efficacious therapy, the recognition that HSV may cause life-threatening infections in neonates and immunocompromised patients, the observation that genital herpes is one of the commonest viral sexually transmitted diseases and the possible association of HSV with cervical cancer.