Infrared spectra of aqueous NO2( - ) and NO3( - ) solutions, and of binary mixtures, were recorded using internal reflection techniques and a Fourier transform spectrometer. NO2( - ) could be detected at a concentration of 0.02 M, and NO3( - ) at 0.001 M. However, NO3( - ) also became adsorbed on the Ge prism. After making corrections for adsorption and for overlapping of NO2( - ) and NO3( - ) bands, it was possible to analyze binary mixtures with concentrations as low as NO3( - ) = 0.03 M and NO2( - ) = 0.05 M. By adjusting the optics so that the usually totally absorbing water stretching band had a transmittance of about 50%, it was possible to observe N-H and C-H stretching bands of solutes. Dissolved materials cause distortions of the background which may influence qualitative and quantitative observations.

A comprehensive handbook outlining state-of-the-art analytical techniques used in geomicrobiology, for advanced students, researchers and professional scientists.

A bestselling classic reference, now expanded and updated to cover the latest instrumentation, methods, and applications The Second Edition of Fourier Transform Infrared Spectrometry brings this core reference up to date on the uses of FT-IR spectrometers today. The book starts with an in-depth description of the theory and current instrumentation of FT-IR spectrometry, with full chapters devoted to signal-to-noise ratio and photometric accuracy. Many diverse types of sampling techniques and data processing routines, most of which can be performed on even the less expensive instruments, are then described. Extensively updated, the Second Edition: * Discusses improvements in optical components * Features a full chapter on FT Raman Spectrometry * Contains new chapters that focus on different ways of measuring spectra by FT-IR spectrometry, including fourteen chapters on such techniques as microspectroscopy, internal and external reflection, and emission and photoacoustic spectrometry * Includes a new chapter introducing the theory of vibrational spectrometry * Organizes material according to sampling techniques Designed to help practitioners using FT-IR capitalize on the plethora of techniques for modern FT-IR spectrometry and plan their experimental procedures correctly, this is a practical, hands-on reference for chemists and analysts. It's also a great resource for students who need to understand the theory, instrumentation, and applications of FT-IR.