This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond. Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Discover important lessons learned about whole organism biology via microbial proteomics This text provides an exhaustive analysis and presentation of current research in the field of microbial proteomics, with an emphasis on new developments and applications and future directions in research. The editors and authors show how and why the relative simplicity of microbes has made them attractive targets for extensive experimental manipulation in a quest for both improved disease prevention and treatment and an improved understanding of whole organism functional biology. In particular, the text demonstrates how microbial proteomic analyses can aid in drug discovery, including identification of new targets, novel diagnostic markers, and lead optimization. Each chapter is written by one or more leading experts in the field and carefully edited to ensure a consistent and thorough approach throughout. Methods, technologies, and tools associated with the most promising approaches are stressed. Key topics covered include: Microbial pathogenesis at the proteome level Whole cell modeling Structural proteomics and computational analysis Biomolecular interactions Physiological proteomics Metabolic reconstruction using proteomics data While presenting the practical utility of proteomics data, the text is also clear on the field's current limitations, pointing to areas where further investigation is needed. Offering a state-of-the-art perspective from internationally recognized experts, this text is ideally suited for researchers and students across the gamut of genomic sciences, including biochemistry, microbiology, molecular biology, genetics, biomedical and pharmaceutical sciences, biotechnology, and veterinary science.

Genomics and genome technology is having, and continues to have, a major impact on all areas of bioscience research providing insights into the key area of molecular mechanisms of cells in health and disease. This is causing a profound effect on biomedical science and is accelerating the development of new diagnostic applications. This book provides a timely, graduate level introduction to the fast-paced area of genomics and clinical diagnostic technologies and introduces the concept of applications based on this area. The initial chapters focus on principal molecular technologies that underpin the information in the later chapters. In addition to introductory areas of nucleic acids and techniques in molecular biology, bioinformatics and proteomics, other key diagnostic areas such as the use of immunological reagents are covered. The later chapters provide more specialised examples of currently used diagnostic technologies and insights into selected key diagnostic challenges including specific examples of molecular microbial diagnostics and molecular biomarkers in oncology. The running themes through the chapters provides an insight into current and future perspectives in this rapidly evolving field.

This volume brings current knowledge of proteomics technologies and related developments with special reference to diseases caused by microbes. The editor has compiled chapters written by expert academicians which distill the information about useful methods in microbial proteomics for the benefit of readers. Chapters cover several methods used to investigate the microbial proteome and special topics such as antimicrobial drug resistance mechanisms, biomarker developments, post translational modifications. Key Features: -overview of several biochemical methods in proteomics -full-color, high quality images of the most frequent technologies and applications -concise, well organized, and didactic format -updates in basic applied information -bibliographic references -information on proteomics for tuberculosis treatment This reference work is intended for researchers seeking information on laboratory techniques applied in proteomics research and microbiology.

New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.

Recent Developments in Applied Microbiology and Biochemistry, Vol. 2, provides a comprehensive treatment and understanding on application oriented microbial concepts, giving readers insights into recent developments in microbial biotechnology and medical, agricultural and environmental microbiology. - Discusses microbial proteome analyses and their importance in medical microbiology - Explores emerging trends in the prevention of current global health problems, such as cancer, obesity and immunity - Shows recent approaches in the production of novel enzymes from environmental samples by enrichment culture and metagenomics approaches - Guides readers through the status and recent developments in analytical methods for the detection of foodborne microorganisms

Introduction to forensic proteomics -- A proteomics tutorial -- Proteomic sample preparation techniques : toward forensic proteomic applications -- NextGen serology : leveraging mass spectrometry for protein-based human body fluid identification -- Informatics approaches to forensic body fluid identification by proteomic mass spectrometry -- Fingermarks as a new proteomic specimen : state of the art and perspective of in situ proteomics -- Human identification using genetically variant peptides in biological forensic evidence -- Proteomics in the analysis of forensic, archaeological, and paleontological bone -- Proteomics for microbial forensics -- ISO 17025 accreditation of method-based mass spectrometry for bioforensic analyses -- Unambiguous identification of ricin and abrin with advanced mass spectrometric assays -- Challenges in the development of reference materials for protein toxins -- The statistical defensibility of forensic proteomics.

This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade. This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss: Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant Anaerobes Identification of Species in Mixed Microbial Populations Detection of Resistance Mechanisms Proteomics as a biomarker discovery and validation platform Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

Dr. Joshua Lederberg - scientist, Nobel laureate, visionary thinker, and friend of the Forum on Microbial Threats - died on February 2, 2008. It was in his honor that the Institute of Medicine's Forum on Microbial Threats convened a public workshop on May 20-21, 2008, to examine Dr. Lederberg's scientific and policy contributions to the marketplace of ideas in the life sciences, medicine, and public policy. The resulting workshop summary, Microbial Evolution and Co-Adaptation, demonstrates the extent to which conceptual and technological developments have, within a few short years, advanced our collective understanding of the microbiome, microbial genetics, microbial communities, and microbe-host-environment interactions.