Eosinophil Ultrastructure: Atlas of Eosinophil Cell Biology and Pathology entirely focuses on eosinophils and their functional roles in inflammation, host defense, and normal homeostatic activities. The book explores the ultrastructure of human eosinophils, highlighting biological processes observed under normal, experimental, and pathological conditions. Created to fill a void in the eosinophil literature, the book includes an extensive array of electron microscopic images that illustrate the diversity of eosinophil morphology. While the atlas is a learning and teaching tool, it is mainly a helpful resource for researchers to identify distinguishing features and structural changes that arise during studies of human eosinophils. The book also covers the ultrastructure of mouse eosinophils under normal and activation conditions and in the context of representative diseases. - Gives guidelines to understand the human eosinophils in studies focused on structural biology, cellular immunology, innate and adaptive immunity, immune responses to pathogens, immunopathology, and inflammatory responses - Provides a core of essential knowledge to identify both immature and mature eosinophils - Comprises a representative compilation of the eosinophil ultrastructure during biological processes, such as activation and degranulation, mostly under experimental conditions - Highlights eosinophil biological processes found in vivo during human diseases, thus providing a link between basic science and clinical aspects - Helps identify distinguishing features and structural changes that arise during studies of human eosinophils after isolation from body fluids, while in cultures, or biopsies - Explains the ultrastructural organization of mature and immature mouse eosinophils, highlighting the similarities/differences between them and human eosinophils

Biological Ultrastructure focuses on the ultrastructure of biological systems, including microscopy and the roles of amino acids, lipoproteins, and carbohydrates. The manuscript first offers information on the methods in ultrastructural research and the principles of molecular structure. Topics include application of electron microscopy and x-ray diffraction, electronic theory of valency, spatial arrangement, and aggregation of molecules and interaction with water. The book then examines the role of proteins and lipids, including amino acids, polypeptide chains, natural lipoproteins, and structure and properties of isolated lipid molecules. The publication underscores the role of carbohydrates and nucleic acids, as well as monosaccharides, disaccharides, polysaccharides, structure of nucleic acids, viruses, and cell nucleus chromosomes and genes. The text also takes a look at the role of ultrastructure in biology and medicine and the role of mineral salts. The manuscript is a valuable source of data for readers interested in biological ultrastructure.

Engström-Finean Biological Ultrastructure, Second Edition, looks upon biological phenomena essentially as patterns of physical and chemical events organized in space and time. It attempts to describe the spatial aspects of this organization, emphasizing in particular the fundamental roles of individual types of molecules. It also introduces a minimum background of fundamental structural information and of techniques so as to aid in the appreciation of the significance of experimental data. The plan of the book in the present edition remains unchanged but the tremendous advances of recent years have necessitated changes in emphasis in the treatment of methods and fundamentals of structure and a complete reappraisal of biological ultrastructure. Key topics discussed include methods in ultrastructural research, the principles of molecular structure; the role of proteins, nucleic acids, lipids, carbohydrates, mineral salts; and the role of ultrastructure in biology and medicine.

Ultrastructure in Biological Systems, Volume 6: Ultrastructure of the Mammalian Heart focuses on the mammalian heart with some cross-reference to that of other vertebrates, such as birds. This book is divided into four main topics— ultrastructure of ventricular and atrial myocardium; impulse generation and conduction system of the heart; embryologic development of the mammalian heart; and ultrastructure of the innervation of the mammalian heart. In these topics, this publication specifically discusses the sarcotubular system (SR), contractile apparatus, general description of cellular morphology, and physiologic implications of cellular and fiber structure. The origin and cytodifferentiation of heart muscle cells, innervation of the sino-atrial node, and ultrastructure of the sympathetic cervical ganglion are also deliberated. This volume is a good source for biologists and students researching on the ultrastructure of the mammalian heart.

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Represents the latest release in the Methods in Cell Biology series - Includes the latest information on Expansion Microscopy for Cell Biology

A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

Three-Dimensional Ultrastructure in Biology

Ultrastructure in Biological Systems, Volume 2: Ultrastructure of the Kidney provides an overview of the state of knowledge on the ultrastructure of the mammalian kidney. The application of the electron microscope to studies of the kidney resulted in the demonstration of the hitherto undetected early thickening of the basement membrane of glomerular capillaries in glomerulonephritis. Yet many problems remain, particularly in relation to the correlation between function and the ultrastructure of components of the kidney—mesangium, glomerulus, juxtaglomerular apparatus, and the renal tubules. It is only recently that the mesangium has come to be accepted as real, and many questions remain as to the function of its cells. The existence of true membranes between foot processes of the epithelial cells of glomeruli is a newly established fact; but what this has to do with glomerular filtration is not known at present. Granules apparently secretory in nature have been identified in cells of the juxtaglomerular apparatus, but so far their presence has not been correlated with specific functional change. Artifacts introduced at fixation are now known to have considerable relevance in interpreting the ultrastructure of the normal nephron. These are paraphrased views of the contributors to this monograph who, while acquainting the reader with the research being carried on in these areas, have also brought into focus the many problems still awaiting solution.