Thin layer chromatography (TLC) is well suited for performing enantioseparations for research as well as larger-scale applications. A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first bo

Thin layer chromatography (TLC) is well suited for performing enantioseparations for research as well as larger-scale applications. A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first book to focus solely on the theory, capabilities, and applications of TLC for direct and indirect enantioseparations. The first part of the book examines the fundamental principles of chirality and TLC. It describes the necessary materials, laboratory equipment, procedures, and strategies for the separation, quantification, isolation, and analysis of chiral compounds.

Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation. The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market—the presence of substandard and counterfeit pharmaceutical products. Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.

"The problem addressed by this dissertation is the separation of optical isomers in commercial as well as biological samples. The chromatographic separation of enantiomers is an important and rapidly developing field of study. Chiral separations of pharmaceutical compounds and important organic intermediates in high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) were achieved. Two methods were employed for the direct liquid chromatographic resolution of chiral analytes: chiral stationary phases (CSPs) and chiral mobile phase additives (CMAs). Native and derivatized [beta]-cyclodextrins ([beta]-CD) were used as chiral stationary phases in reverse phase and normal phase HPLC, respectively. This study marked the first use of derivatized [beta]-CDs for chiral separations in normal phase media. N-carbobenzoxy-glycl-L-proline and (1R)-(-)ammonium-10-camphorsulfonate were utilized as CMAs in normal phase TLC for the resolution of several aromatic amino alcohols. Maltosyl-[beta]-CD and hydroxypropyl-[beta]-CD were employed as CMAs in reverse phase TLS. A study was conducted with hydroxypropyl-[beta]-CD to determine how the degree of substitution of a derivatized CD could effect development time, the viscosity of the solution and the enantioselectivity. In addition, studies were initiated to determine the presence of trace levels of D-amino acids in: amniotic fluid, blood serum and urine. The blood and urine of healthy young adults were analyzed and found to contain trace to percent levels of D-amino acids. The human amniotic fluid samples did not have detectable levels of D-amino acids"--Abstract, page iv.

This volume represents the proceedings of a two-day international meeting on chiral chromatography held at the University of Surrey between 3-4 September 1987. The meeting was jointly organized by the Chromatographic SOCiety and the Robens Institute of the University of Surrey in response to the burgeoning interest in this rapid maturing field of chromatography. Nowhere is this interest more evident than in the agrochemical and pharmaceutical industries where the implications of different pharmacological and toxicological activity for the individual enantiomers present in a racemic drug ol" insecticide is an increasing area of concern. Developments in the area of chiral separations are at last beginning to provide SCientists with the necessary tools to study how animals and man handle racemates and relate their obseIVations to the obseIVed biological effects of these substances. The development of robust and Simple methods for the separation of enantiomers will therefore have a profound Impact on safety evaluation and drug design. The meeting proved to be very successful. with over 160 delegates from thirteen countries in Europe and America present to learn from the experiences of experts in the field of chiral chromatography and to hear about the latest developments. Hopefully. in future symposia on chiral separations at the University of Surrey.

In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques. This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography. Features individual chapters written by recognized authoritative and visionary experts in the field Provides an overview and focused treatment of a single topic Provides tables and diagrams with commonly used data to facilitate practical work, comparison of results, and decision-making Places modern developments in the research literature into a general context not always apparent to inexperienced users of the technique Offers comprehensive updates to all chapters Includes new chapters on instrument platforms, effects-directed detection, data analysis tools, small-scale and microfluidic planar separation systems, and applications to the separation of amino acids and peptides, the analysis of saccharides and lipids, and forensic analysis

Eine Ergänzung anderer, in der Reihe Separation Sciences bereits erschienener Titel. Viel Theorie und zahlreiche Anwendungsbeispiele für die Praxis der chiralen HPLC- und GC-Trennung machen dieses top-aktuelle Buch zu einer unverzichtbaren Informationsquelle für jeden Neueinsteiger auf diesem Gebiet. (11/98)

Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.

This title discusses all phases of thin-layer chromatography (TLC), including the preparation of biological samples prior to TLC, general practices of TLC, and applications based on compound types. Stresses practical rather than theoretical aspects of TLC, compares modern TLC with other separation methods, contains extensive coverage of sample preparation methods, surveys the latest instrumentation for high-performance and overpressured TLC, covers current principles and techniques, and more.