The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.

The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.

Seafood and seafood products represent some of the most important foods in almost all types of societies around the world. More intensive production of fish and shellfish to meet high demand has raised some concerns related to the nutritional and sensory qualities of these cultured fish in comparison to their wild-catch counterparts. In addition, the variety in processing, preservation, and storage methods from traditional to modern is contributing to an increase in variability in consumer products. This second edition of the Handbook of Seafood and Seafood Products Analysis brings together the work of 109 experts who focus on the most recent research and development trends in analytical techniques and methodologies for the analysis of captured fresh and preserved seafood, either cultivated or wild, as well as for derived products. After providing a general introduction, this handbook provides 48 chapters distributed in six sections: Chemistry and biochemistry focuses on the analysis of main chemical and biochemical compounds of seafood. Processing control describes the analysis of technological quality and the use of some non-destructive techniques as well as methods to check freshness, detection of species, and geographic origin and to evaluate smoke flavoring. Nutritional quality deals with the analysis of nutrients in seafood such as essential amino acids, bioactive peptides, antioxidants, vitamins, minerals and trace elements, and fatty acids. Sensory quality covers the sensory quality and main analytical tools to determine color, texture, flavor and off-flavor, quality index methods as well as sensory descriptors, sensory aspects of heat-treated seafood, and sensory perception. Biological Safety looks at tools for the detection of spoilage, pathogens, parasites, viruses, marine toxins, antibiotics, and GM ingredients. Chemical Safety focuses on the identification of fish species, detection of adulterations, veterinary drug residues, irradiation, food contact materials, and chemical toxic compounds from the environment, generated during processing or intentionally added. Key Features: This comprehensive handbook provides a full overview of the tools now available for the analysis of captured fresh and preserved seafood, either cultivated or wild, as well as for derived products. This is a comprehensive and informative book that presents both the merits and limitations of analytical techniques and also gives future developments for guaranteeing the quality of seafood and seafood products. This cutting-edge work covers processes used from all of the seven seas to ensure that consumers find safe, nutritionally beneficial, and appealing seafood products at their markets and restaurants. This handbook covers the main types of worldwide available analytical techniques and methodologies for the analysis of seafood and seafood products.

The book is a multidisciplinary space and serves as a platform to share and learn about the frontier knowledge between different areas related to “Recent trends in sustainable engineering.” Sustainable engineering promotes the responsible use of resources and materials involved in the different manufacturing processes or the execution stages of a service. An interdisciplinary approach is required in all aspects of engineering. In this sense, engineers, researchers, and the academic community will play a fundamental role in developing new technologies that respect the environment, still, at the same time, that considers social and economic factors.

This book describes the principles of the bioanalytical techniques that are used in clinical diagnosis, pharmaceutical analysis, bioprocess monitoring, environmental studies and life sciences research. The primary focus of this book is on instrumental methods which are evolving rapidly due to the availability of new technologies. The basic concepts of each technique are explained in as concise and simple a manner as possible, without sacrificing generality or rigour. Part-I and Part-II are primarily for undergraduate students. Part-I describes analytical methods that are based on the separation of particles (chromatography, electrophoresis, mass spectrometry, etc). Part-II describes methods based on spectroscopy, scattering and diffraction. Part-III introduces some basic biochemical and molecular biological methods such as binding assays, PCR, protein sequencing and nucleic acid sequencing and is intended as background material for students who are interested in the topics described in Part-IV. Part-IV describes the basic concepts of novel micro- and nano-bioanalytical techniques which are based on biosensors, microarrays, microfluidics, surface plasmon resonance and nanomaterials. Part-IV is primarily for postgraduate students. However, adequate basics are provided to ensure that advanced undergraduates can also benefit from this section.

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Chemical engineering applications have been a source of challenging optimization problems in terms of economics and technology. The goal of this book is to enable the reader to get instant information on fundamentals and advancements in chemical engineering. This book addresses ongoing evolutions of chemical engineering and provides overview to the sate of the art advancements. Molecular perspective is increasingly important in the refinement of kinetic and thermodynamic molding. As a result, much of the material was revised on industrial problems and their sophisticated solutions from known scientists around the world. These issues were divided in to two sections, fundamental advances and catalysis and reaction engineering. A distinct feature of this text continues to be the emphasis on molecular chemistry, reaction engineering and modeling to achieve rational and robust industrial design. Our perspective is that this background must be made available to undergraduate, graduate and professionals in an integrated manner.

Food proteins and bioactive peptides play a vital role in thegrowth and development of the body’s structural integrity andregulation, as well as having a variety of other functionalproperties. Land animal-derived food proteins such as collagen andgelatine carry risks of contamination (such as BSE). Marine-derivedproteins, which can provide equivalents to collagen and gelatinwithout the associated risks, are becoming more popular amongconsumers because of their numerous health beneficial effects. Mostmarine-derived bioactive peptides are currently underutilized.While fish and shellfish are perhaps the most obvious sources ofsuch proteins and peptides, there is also the potential for furtherdevelopment of proteins and peptides from sources like algae, seacucumber and molluscs. Marine-derived proteins and peptides alsohave potential uses in novel products, with the possibility of widecommercialization in the food, beverage, pharmaceutical andcosmetic industries, as well as in other fields such asphotography, textiles, leather, electronics, medicine andbiotechnology. Marine Proteins and Peptides: Biological Activities andApplications presents an overview of the current status,future industrial perspectives and commercial trends of bioactivemarine-derived proteins and peptides. Many of the industrialperspectives are drawn from the food industry, but the book alsorefers to the pharmaceutical and cosmetics industries. There haverecently been significant advances in isolating functionalingredients from marine bio-resources and seafood by-products foruse in these industries, but little has been published, creating aknowledge gap, particularly with regard to the isolation andpurification processes. This book is the first to fill thatgap. Marine Proteins and Peptides: Biological Activities andApplications is a valuable resource for researchers inmarine biochemistry field as well as food industry managersinterested in exploring novel techniques and knowledge onalternative food protein sources. It will become a standardreference book for researchers involved in developing marinebio-resources and seafood by-products for novel nutraceutical,cosmetics, and pharmaceutical applications. It will also appeal tomanagers and product developers in the food, pharmaceutical andcosmetics industries, particularly those looking to usemarine-derived proteins and peptides as substitutes or replacementsfor unfashionable or outdated food components.

The marine environment covers 70% of the earth’s surface and accounts for 98% of the potentially habitable space. The bioactives from marine microorganisms include antibiotic compounds, polysaccharides, inhibitors, enzymes, peptides, and pigments. These are used in various fields of biology that range from nutraceuticals to cosmeceuticals. Recent scientific investigations have revealed that marine microbial compounds exhibit various beneficial biological effects, such as anti-inflammatory, anti-cancer, anti-HIV, anti-hypertensive, and anti-diabetic. Marine Microorganisms: Extraction and Analysis of Bioactive Compounds sheds light on the extraction, clean-up, and detection methods of major compounds from marine organisms. The book includes information on the different classes of marine microorganisms and the different bioactives that can be extracted from bacteria, fungi and microalgae. Divided into 7 chapters, the book covers bioactive marine natural products, such as marine microbes, seaweeds, and marine sponges as potential sources of drug discovery, and focuses on analysis methods of the biocomponents from marine microorganisms. A useful reference tool for researchers and students, this book provides current knowledge about isolation and analysis methods of the bioactives and provides insight into the various bioactives of marine microbes toward nutraceutical and pharmaceutical development.