Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings.

History of Harrison County, Missouri containing personal sketches of many who have been identified with the development the county.

For two hundred years the pessimists have dominated public discourse, insisting that things will soon be getting much worse. But in fact, life is getting better—and at an accelerating rate. Food availability, income, and life span are up; disease, child mortality, and violence are down all across the globe. Africa is following Asia out of poverty; the Internet, the mobile phone, and container shipping are enriching people's lives as never before. In his bold and bracing exploration into how human culture evolves positively through exchange and specialization, bestselling author Matt Ridley does more than describe how things are getting better. He explains why. An astute, refreshing, and revelatory work that covers the entire sweep of human history—from the Stone Age to the Internet—The Rational Optimist will change your way of thinking about the world for the better.

Excerpt from History of Eau Claire County, Wisconsin, Past and Present: Including an Account of the Cities, Towns and Villages of the County The publishers of the history desire to acknowledge the cor dial and valuable assistance which has been accorded them in its compilation by many citizens of Eau Claire county. It has been a help deeply appreciated and deserves due recognition. Among those to whom special thanks are due is Hon. William F. Bailey, James H. Waggoner, Percy C. Atkinson, Marshall Cousins, Walde mar Ager, Reinhold Liebau, Miss A. E. Kidder, W. H. Schulz, W. W. Bartlett, L. A. Brace, J. P. Welsh, Frank L. Clark, C. W Lockwood, G. Caldwell, W. A. Clark. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.

This handbook contains 13 chapters covering banana (Musa and Ensete) diseases caused by various groups of causal agents and disorders caused by unknown and known factors. Topics discussed include fungal diseases of the foliage, root, corm, pseudostem, fruit (pre- and postharvest), as well as diseases caused by bacteria and phytoplasmas, viruses and nematode pathogens. Information is given on their economic impact, distribution, symptoms, disease cycle and epidemiology, host reaction, and control. Non-infectious disorders, mineral deficiencies, injuries caused by adverse climate and extreme weather, chemical injuries and genetic abnormalities are also described. Topic on quarantine and the safe movement of Musa germplasm is also given. This book, like the last, is for all 'banana doctors' around the world. It is hoped that it serves as a useful field and laboratory guide plus a source of information to all those investigating problems of the banana, abacá and enset crops.