Substitute components in the Abernathy salmon diet were tested in 2 years of feeding trials with fall chinook salmon fingerlings. These diet tests indicated that turbot meal and dogfish meal were adequate substitutes for salmon meal. Soybean oil was superior to peanut, corn, cottonseed, or safflower oil. Corn gluten, safflower or soybean meals were inadequate substitutes for cottonseed meal. Dried buttermilk was equal to dried skim milk. The mixed diet could be stored under refrigeration for 3 days and the premixed meals could be held at room temperature for several weeks without observable deleterious effect on the fish; storage for greater periods was deleterious.

The 1970 fall chinook salmon feeding trials indicated that dry pelleted diets were equal to moist pelleted diets with similar formulations. A superior diet was produced by reducing the ratio of dried whey product and wheat germ meal to 1:1 and eliminating cottonseed meal. This formulation feed at a 45-percent protein level was more efficient than other protein levels fed. Growth was not reduced when the soybean oil supplement was lowered from 6 to 2 percent of the diet. Two corn distillers' products that were substituted as partial replacements for dried whey product did not enhance fish growth. Storage of the Abernathy dry pellet at room temperature did not alter the nutritional adequacy of the diet.

Feeding trials using fall chinook salmon finglerlings were conducted at the Salmon Cultural Laboratory, Longview, Washington, during 1971 for the purpose of improving the Abernathy diet formula. The results indicated that cottonseed meal could replace a portion of the fish meal in the diet without reducing fish growth, but similar substitutions of wheat and corn gluten meal reduced growth. Fish growth was significantly increased when a diet containing 50 percent protein and 3,350 kcal per kilogram was fed as compared with a diet containing 45 percent protein and 3,350 kcal per kilogram. Soybean lecithin proved to be equal to soybean oil as a caloric source when fed at 2 percent of the diet. Two types of dried whey product with different levels of lactose content produced similar growth response. Reducing the dried whey portion of the diet to 5 percent did not affect growth, nor did methionine supplementation produce any effects. Anchovy meal was unsuitable as a replacement for herring meal.

The 1966 and 1967 diet trials at the Salmon-Cultural Laboratory indicated that fall chinook salmon fingerlings utilized an all-meal diet more efficiently when pelleted than when ricer feed. The Abernathy pellet was comparable to the Oregon pellet. Supplement vitamins C and E proved to be required under certain conditions of the experiment while vitamins K, para-aminobenzoic acid, B12, cod liver oil, and a mineral mixture were not shown to be of benefit.

Studies on two hemoflagellates of fish were carried out to determine biological parameters: host range, vector relations, in vivo culture, in vitro culture, pathogenicit, and cryogenic preservation.

Static bioassays were conducted to determine the relative acute toxicites of some insecticides, herbicides, fungicides, and a defoliant to the scud Gammarus lacustris.