Studies in Clinical Enzymology presents a critical review of the nature and diagnostic value of enzymes. It discusses the characteristics of iso-enzymes; the enzymes changes in myocardial infarction, cardiomyopathies, and non-ischemic heart failure; and the characteristics of enzymes studied in African heart disease. Some of the topics covered in the book are the simple enzyme kinetics; classification of enzymes; description of activators and co-enzymes; mechanics of enzyme inhibition; effects of drugs in enzyme reactions; function of the cell membrane; distribution of enzymes; mechanism of enzyme increase; and enzyme changes in disease. The causes of increased phosphatase activity and the evaluation of molecular theory are discussed. An in-depth analysis of the use of iso-enzymes to determine the source of increased serum enzyme activity in disease is provided. The enzyme changes in congestive heart failure not due to myocardial infarction are presented. A chapter is devoted to the liver function and enzyme changes in hepatic amoebiasis. The book can provide useful information to cardiologists, biologists, students, and researchers.

This book is the 2nd improved and expanded edition of "Clinical Enzymology" (Lott/Wolf, 1987). It includes case studies and guidelines for specialists of laboratory medicine and clinicians, devotes each chapter to a specific enzyme or protein marker, contains case studies and guidelines, a section on marker biochemistry and physiology as well as a section on special pathology and analysis. The clear, didactic structure and the multiple choice questions also make the book valuable reading for graduate students in the fields of clinical pathology and laboratory medicine.

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course. - Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject - Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification - Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy