Soil enzymes are one of the vital key mediators involved in nutrient recycling and the decomposition of organic matter and thereby in maintaining soil quality and fertility. This Soil Biology volume covers the various facets of soil enzymes, such as their functions, biochemical and microbiological properties and the factors affecting their activities. Enzymes in the rhizosphere, in forest soils, and in volcanic ash-derived soils are described. Soil enzymes covered include phosphohydrolases, lignocellulose-degrading enzymes, phenol oxidases, fungal oxidoreductases, keratinases, pectinases, xylanases, lipases and pectinases. Several chapters treat the soil enzymatic activities in the bioremediation of soils contaminated with pesticides and pollutants such as oil, chlorinated compounds, synthetic dyes and aromatic hydrocarbons. The role of soil enzymes as bioindicators is a further important topic addressed.

Methods of Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Readers will especially benefit from the step-by-step explanation of the lab procedures, as well as background information for using these methods effectively and analyzing data. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background informaton, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. Written for the researcher, but welcoming to those new to soil enzymology, the introduction includes conceptual information to orient those who are not familiar with these methods but want to use them. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.

Soil sampling for microbiological analysis; Statistical treatment of microbial data; Soil sterilization; Soil water potencial; Most probable number counts; Light microscopic methods for studying soil microorganisms; Viruses; Recovery and enumeration of viable bacteria; Coliform bacteria; Autotrophic nitrifying bacteria; Free-living dinitrogen-fixing bacteria; Legume nodule symbionts; Anaerobic bacteria and processes; Denitrifiers; Actiomycetes; Frankia and the actinorhizal symbiosis; Filamentous fungi; Vesicular-arbuscular mycorrhizal fungi; Isolation of microorganisms producting antibiotics; Microbiological procedures for biodegradation research; Algae and cyanobacteria; Marking soil bacteria with lacZY; Detection of specific DNA sequences in environmental sample via polymerase chaim reaction; Isolation and purification of bacterial DNA from soil; Microbial biomass; Soil enzymes; Carbon mineralization; Isotopic methods for the study of soil organic matter dynamics ; Practical considerations in the use of nitrogen tracers in agricultural and environmental research; Nitrogen availability; Nitrogen mineralization, immobilization, and nitrification; Dinitrogen fixation; Measuring denitrification in the field; Sulfur oxidation and reduction in soils; Iron and manganese oxidation and reduction.

Soil enzymes play a fundamental role in many soil processes such as the mineralization of organic matter, the synthesis of humic substances, the degradation of xenobiotics or the mechanisms involved in the biocontrol of plant pathogens. Their direct link with soil microorganisms gives them a key role as biomonitors of the evolution of soil quality or in the monitoring of the application of organic amendments to degraded soils. As a consequence of the importance of soil enzymes on soil processes, there is an increasing interest in their study, as well as in the application of molecular techniques as diagnostic tools.

The need to understand the biological processes that are important for essential aquatic and terrestrial ecosystem function has prompted much research into the field of ecological enzymology. This book presents the two broad areas of application in a compilation of reviews by 21 international experts in their respective fields. The first explores enzymatic activities to assess the processes or mechanisms that operate in a given system, such as the rhizosphere, plant leaves and shoots, soil surfaces, and biofilms. The second considers enzymes or microbial cells as sensors to detect microbial activity and stresses due to pollution, management, or climatic change in both aquatic and terrestrial ecosystems.

The fourth edition of Soil Microbiology, Ecology and Biochemistry updates this widely used reference as the study and understanding of soil biota, their function, and the dynamics of soil organic matter has been revolutionized by molecular and instrumental techniques, and information technology. Knowledge of soil microbiology, ecology and biochemistry is central to our understanding of organisms and their processes and interactions with their environment. In a time of great global change and increased emphasis on biodiversity and food security, soil microbiology and ecology has become an increasingly important topic. Revised by a group of world-renowned authors in many institutions and disciplines, this work relates the breakthroughs in knowledge in this important field to its history as well as future applications. The new edition provides readable, practical, impactful information for its many applied and fundamental disciplines. Professionals turn to this text as a reference for fundamental knowledge in their field or to inform management practices. - New section on "Methods in Studying Soil Organic Matter Formation and Nutrient Dynamics" to balance the two successful chapters on microbial and physiological methodology - Includes expanded information on soil interactions with organisms involved in human and plant disease - Improved readability and integration for an ever-widening audience in his field - Integrated concepts related to soil biota, diversity, and function allow readers in multiple disciplines to understand the complex soil biota and their function

Soil enzymes are one of the vital key mediators involved in nutrient recycling and the decomposition of organic matter and thereby in maintaining soil quality and fertility. This Soil Biology volume covers the various facets of soil enzymes, such as their functions, biochemical and microbiological properties and the factors affecting their activities. Enzymes in the rhizosphere, in forest soils, and in volcanic ash-derived soils are described. Soil enzymes covered include phosphohydrolases, lignocellulose-degrading enzymes, phenol oxidases, fungal oxidoreductases, keratinases, pectinases, xylanases, lipases and pectinases. Several chapters treat the soil enzymatic activities in the bioremediation of soils contaminated with pesticides and pollutants such as oil, chlorinated compounds, synthetic dyes and aromatic hydrocarbons. The role of soil enzymes as bioindicators is a further important topic addressed.

The disturbance of soils, like other phenomena of environmental pollution, encountered in so many areas all over the world, has become a subject of extensive concern and has led to a vast amount of literature in the field of enzymology, too.The book is the first comprehensive and updated review of the enzymological literature on three great categories of disturbed soils, including their remediation. The volume consists of three sections: I. Enzymology of oil-contaminated soils; II. Enzymology of soils affected by industrial emissions; and III, enzymology of technogenic soils. Within each section the reviewed studies are grouped by country.The volume will be of great value to soil scientists, environmental scientists, conservationists, and many other scientists in these and related fields.

Extracellular Enzymes in Environments: Responses to Collaborative Remediation of Contaminated Soil and Groundwater provides an overview of the functions, activities, and analysis methods of enzymes in soil and water environments. In addition, the response of enzymes to environmental changes (e.g., contamination, remediation, climate change, fertilization) is also summarized based on experimental results. Spatial and temporal distribution of enzyme activities is assessed using in-situ zymography. Furthermore, variation of enzyme activities in hotspots (i.e., rhizosphere, detritusphere) and controlling factors are also summarized. - Provides detailed information on the in-situ zymography technique, which can visualize enzyme activities in soil and water environments - Quantifies distribution of enzyme activities in response to nano-metal oxides contamination and use of enzymes to evaluate remediating effect of various carbon-based biomass materials on heavy metal contamination - Covers the responses of enzymes to environmental changes (e.g., contamination, remediation, climate change, and fertilization) are summarized

History of abiontic soil ensyme research; Origin and range of enzymes in soil; kinetics and consecutive reactions of soil enzymes; Soil polysaccharidases: activity and agricultural importance; Urease activity in soils; Soil phosphatase and sulphatase; Interactions between agrochemicals and soil enzymes; Enzyme activity in soil: some theoretical and practical considerations; Methodology of soil enzyme measurement and extraction.