Small Molecule Microarrays: Methods and Protocols
Author | : Mahesh Uttamchandani |
Publisher | : Methods in Molecular Biology |
Total Pages | : 274 |
Release | : 2018-12-05 |
ISBN-10 | : 1493982427 |
ISBN-13 | : 9781493982424 |
Rating | : 4/5 (27 Downloads) |
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Author | : Mahesh Uttamchandani |
Publisher | : Methods in Molecular Biology |
Total Pages | : 274 |
Release | : 2018-12-05 |
ISBN-10 | : 1493982427 |
ISBN-13 | : 9781493982424 |
Rating | : 4/5 (27 Downloads) |
Author | : Marina Cretich |
Publisher | : Springer Nature |
Total Pages | : 261 |
Release | : 2022-09-24 |
ISBN-10 | : 9781071627327 |
ISBN-13 | : 1071627325 |
Rating | : 4/5 (27 Downloads) |
This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe full coverage of the aspects related to their workflows, from microchip manufacturing to advanced analytical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peptide Microarrays: Methods and Protocols, Third Edition aims to make the use of peptide microarrays more and more accessible while stimulating further developments to sustain exciting discoveries in the biochemistry and medicine realms.
Author | : Ella Palmer |
Publisher | : Humana Press |
Total Pages | : 0 |
Release | : 2010-11-24 |
ISBN-10 | : 1617379697 |
ISBN-13 | : 9781617379697 |
Rating | : 4/5 (97 Downloads) |
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
Author | : Robert S. Matson |
Publisher | : CRC Press |
Total Pages | : 216 |
Release | : 2009-01-20 |
ISBN-10 | : 9781420046663 |
ISBN-13 | : 1420046667 |
Rating | : 4/5 (63 Downloads) |
A Step-by-Step Guide to Present and Future Uses of Microarray TechnologyMicroarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts
Author | : Ulrike Korf |
Publisher | : Humana Press |
Total Pages | : 0 |
Release | : 2011-08-19 |
ISBN-10 | : 1617792853 |
ISBN-13 | : 9781617792854 |
Rating | : 4/5 (53 Downloads) |
Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable. In Protein Microarrays: Methods and Protocols, expert researchers in the field present an up-to-date collection of robust strategies in the field of protein microarrays and summarize recent advantages in the field of printing technologies, the development of suitable surface materials, as well as detection and quantification technologies. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Protein Microarrays: Methods and Protocols aims to stimulate the application and further advancement of this powerful technology in labs worldwide.
Author | : Ali Khademhosseini |
Publisher | : Humana Press |
Total Pages | : 0 |
Release | : 2010-10-28 |
ISBN-10 | : 1934115959 |
ISBN-13 | : 9781934115954 |
Rating | : 4/5 (59 Downloads) |
Recent developments in microarray technology have changed the landscape of biology and biomedical research, and they have revolutionized RNA and DNA research. In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods. Chapters address both the application of biological microarrays, including DNA/RNA, apatmer, proteins, tissues, oligonucleotides, carbohydrates, biomaterials, cells, bacteria, and virus microarrays, and also explore the different techniques used for generating microarray platforms. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and revolutionary, Biological Microarrays: Methods and Protocols serves as a primary source for academics, practitioners, and professionals in related fields, including biologists, biotechnologists, biochemists, analytical chemists, and biomedical, physical, and microsystems engineers, to name a few, appealing to all of those interested in the present and future state of biological microarray research.
Author | : Marina Cretich |
Publisher | : |
Total Pages | : 298 |
Release | : 2016 |
ISBN-10 | : 1493930389 |
ISBN-13 | : 9781493930388 |
Rating | : 4/5 (89 Downloads) |
This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays. Advancements made in recent years in peptide library synthesis, immobilization chemistry, and array production have created a foundation from which different new applications are derived, extending the ways in which peptide microarray technology is applied every day. Divided into three sections, the book covers cutting-edge methods and technology, chemoselective strategies for peptide immobilization, and peptide microarrays for medical applications. Written for the high successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Peptide Microarrays: Methods and Protocols, Second Edition seeks to encourage scientists to apply current peptide array protocols to the study of interesting new biochemical and medical questions and to assist researchers aiming at developing new methods to further develop peptide microarray technology.
Author | : Catherine J. Wu |
Publisher | : Springer Science+Business Media |
Total Pages | : 373 |
Release | : 2011 |
ISBN-10 | : 1617790435 |
ISBN-13 | : 9781617790430 |
Rating | : 4/5 (35 Downloads) |
This comprehensive survey of the field sees expert researchers provide concise descriptions of the most up-to-date methodologies used to fabricate microarrays for the analysis of proteins or responses to proteins that help inform our study of human disease.
Author | : Rodrigo Barderas |
Publisher | : Humana |
Total Pages | : 282 |
Release | : 2022-06-26 |
ISBN-10 | : 1071615645 |
ISBN-13 | : 9781071615645 |
Rating | : 4/5 (45 Downloads) |
This volume focuses on protein analysis, and covers a wide array of uses of protein microarray for disease analysis. The chapters in this book discuss different stages of protein microarrays from their construction to their use, including different types of protein microarrays such as recombinant proteins, antibody, phage, and NAPPA protein microarrays, in planar format or in solution via beads arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Protein Microarrays for Disease Analysis: Methods and Protocols is a valuable resource for graduate and post-doctoral fellows interested in protein microarrays, as well as senior researchers interested in gaining more insight into this developing field.
Author | : Ronald Simon |
Publisher | : Methods in Molecular Biology |
Total Pages | : 212 |
Release | : 2010-08-19 |
ISBN-10 | : STANFORD:36105211750513 |
ISBN-13 | : |
Rating | : 4/5 (13 Downloads) |
Today’s tissue microarray (TMA) method presents as a modern high-tech technology, one which allows for the linking of clinical data to the tissues that are combined on one slide. In Tissue Microarrays: Methods and Protocols, expert researchers explore the current world of TMA making and TMA applications, providing insight into the inherent and complex aspects of the most popular assays used for in-situ tissue analysis. Chapters examine the range of TMA techniques that allow for a large number of tissues to be included in one TMA, preserve the integrity of donor tissue blocks, and present a highly organized array pattern that allows for the reliable allocation of clinical data to individual tissue spots. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and ground-breaking, Tissue Microarrays: Methods and Protocols serves as an essential handbook for pathologists, molecular biologists, researchers in the life sciences, as well as physicians, a reflection of the various applications of current TMA technology.