Reactive Species Detection in Biology: From Fluorescence to Electron Paramagnetic Resonance Spectroscopy discusses the reactive oxygen species that have been implicated in the pathogenesis of various diseases, presenting theories, chemistries, methodologies, and various applications for the detection of reactive species in biological systems, both in-vitro and in-vivo. Techniques covered include fluorescence, high performance chromatography, mass spectrometry, immunochemistry, and electron paramagnetic resonance spectroscopy. Probe design and development are also reviewed in order to advance new approaches in radical detection through synthesis, computations, or experimental applications. - Reviews all current advances in radical detection - Emphasizes chemical structures and reaction schemes fundamental to radical detection and identification - Describes the uses, advantages, and disadvantages of various probe designs - Examines new approaches to radical probe development

This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.

Oxygen represents only 20% of the Earth's atmosphere, yet it is vital for the survival of aerobic organisms. There is a dark part of the use of oxygen that consists in generating reactive species that are potentially harmful to living organisms. Moreover, reactive oxygen species can combine with nitrogen derivatives and generate many other reactive species. Thus, living organisms are continuously assaulted by reactive species from external or internal sources. However, the real danger comes in the case of high concentrations and prolonged exposure to these species. This book presents an image of the mechanisms of action of reactive species and emphasizes their involvement in diseases. Inflammation and cancer are examined to determine when and how reactive species turn the evolution of a benign process to a malignant one. Some answers may come from recent studies indicating that reactive species are responsible for epigenetic changes.

Peroxynitrite is a powerful oxidiser which can damage a wide array of molecules within cells, including DNA and proteins, leading to apoptosis, inflammation or cancer. Peroxynitrite detection and quantification provides critical information in understanding its biological implications. Attempts to investigate the behavior of peroxynitrite in vivo and in vitro have been hampered by the difficulty in detecting this highly reactive oxygen species. This book presents the current state of the art in this research field with contributions from scientific leaders in the field. The chapters make clear the associated challenges and development for selective and sensitive detection of peroxynitrite. This book is a timely addition to the literature, as the first in the field, dedicated to detecting this molecule in vivo. It will be welcomed by the community particularly medicinal and analytical chemists, developers of sensors and probes and analytical equipment manufacturers.

The focus of this collection of illustrated reviews is to discuss the systems biology of free radicals and anti-oxidants. Free radical induced cellular damage in a variety of tissues and organs is reviewed, with detailed discussion of molecular and cellular mechanisms. The collection is aimed at those new to the field, as well as clinicians and scientists with long standing interests in free radical biology. A feature of this collection is that the material also brings insights into various diseases where free radicals are thought to play a role. There is extensive discussion of the success and limitations of the use of antioxidants in several clinical settings.

Nearly a century of scientific research has revealed that mitochondrial dysfunction is one of the most common and consistent phenotypes of cancer cells. A number of notable differences in the mitochondria of normal and cancer cells have been described. These include differences in mitochondrial metabolic activity, molecular composition of mitochondria and mtDNA sequence, as well as in alteration of nuclear genes encoding mitochondrial proteins. This book, Mitochondria and Cancer, edited by Keshav K. Singh and Leslie C. Costello, presents thorough analyses of mitochondrial dysfunction as one of the hallmarks of cancer, discusses the clinical implications of mitochondrial defects in cancer, and as unique cellular targets for novel and selective anti-cancer therapy.

"Oxidative stress" is used as the generic term describing the involve ment of reactive oxygen species in various human diseases. The scope of such a topic is becoming increasingly wide. The recent interest in radicals such as nitric oxide and the discovery of new mechanisms such as the effect of free radicals on redox sensitive proteins and genes are enlarging our understanding of the physiological role of free radicals. Oxidative stress is involved in numerous pathological. processes such as ageing, respiratory or cardiovascular diseases, cancer, neurological pathologies such as dementia or Parkinson's disease. It still remains difficult, however, to demonstrate by chemical measurement the in vivo production of free radicals and even more to realise their speciation. Therefore, the development of new tools and indicators is engrossing many researchers working in this field. Reliable indicators are abso lutely necessary not only to monitor the evolution of oxidative stress in patients but also to evaluate the efficiency of new antioxidant treat ments. The French Free radical club of Grenoble, the CERLIB has been involved for many years in the organisation of international training programs on methodology, in order to provide both theoretical and practical help to researchers from various countries. Such training sessions have been highly successful and participants value the oppor tunity to learn reliable techniques. This positive echo explains why the researchers of CERLIB decided, with the help of Prof. Dr. B. Kalyanaraman, to publish selected techniques on free radical re search.

Presents a multidisciplinary analysis of the integration among reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive sulfur species (RSS). Since plants are the main source of our food, the improvement of their productivity is the most important task for plant biologists. In this book, leading experts accumulate the recent development in the research on oxidative stress and approaches to enhance antioxidant defense system in crop plants. They discuss both the plant responses to oxidative stress and mechanisms of abiotic stress tolerance, and cover all of the recent approaches towards understanding oxidative stress in plants, providing comprehensive information about the topics. It also discusses how reactive nitrogen species and reactive sulfur species regulate plant physiology and plant tolerance to environmental stresses. Reactive Oxygen, Nitrogen and Sulfur Species in Plants: Production, Metabolism, Signaling and Defense Mechanisms covers everything readers need to know in four comprehensive sections. It starts by looking at reactive oxygen species metabolism and antioxidant defense. Next, it covers reactive nitrogen species metabolism and signaling before going on to reactive sulfur species metabolism and signaling. The book finishes with a section that looks at crosstalk among reactive oxygen, nitrogen, and sulfur species based on current research done by experts. Presents the newest method for understanding oxidative stress in plants. Covers both the plant responses to oxidative stress and mechanisms of abiotic stress tolerance Details the integration among reactive oxygen species (ROS), reactive nitrogen species (RNS) and reactive sulfur species (RSS) Written by 140 experts in the field of plant stress physiology, crop improvement, and genetic engineering Providing a comprehensive collection of up-to-date knowledge spanning from biosynthesis and metabolism to signaling pathways implicated in the involvement of RONSS to plant defense mechanisms, Reactive Oxygen, Nitrogen and Sulfur Species in Plants: Production, Metabolism, Signaling and Defense Mechanisms is an excellent book for plant breeders, molecular biologists, and plant physiologists, as well as a guide for students in the field of Plant Science.

Describes the basics of ROS metabolism in plants and examines the broad range of ROS signaling mechanisms New discoveries about the effects of reactive oxygen species (ROS) on plants have turned ROS from being considered a bane into a boon, because their roles have been discovered in many plant developmental processes as signaling molecules. This comprehensive book teaches about the role of ROS metabolism in plants and how they affect various developmental processes. It also discusses in detail the advancements made in understanding the ROS signaling. Reactive Oxygen Species in Plants: Boon Or Bane - Revisiting the Role of ROS begins by presenting the basic introduction to ROS and deciphers the detailed knowledge in ROS research. It then examines the broad range of ROS signaling mechanisms as well as how they may be beneficial for plants and human beings. This book also describes both the bane and boon aspects of ROS with their impact on plants, and how the recent revelations have compelled us to rethink ROS turning from stressors to plant regulators. ● Compiles, for the first time, the wholesome knowledge in ROS research and their cellular signaling ● Includes new discoveries and in-depth discussions about the advancements made in the field ● Discusses reactive oxygen species which are involved in a broad range of biological processes Reactive Oxygen Species in Plants: Boon Or Bane - Revisiting the Role of ROS will help scientists to utilize the functions of ROS signaling for plants and also enable readers to gain a deeper knowledge of ROS research and signaling. It is highly recommended for researchers, scientists, and academicians in plant science as well for advanced undergraduate and postgraduate students.