Rapid Purification and Analysis of RNA Secondary Structures
Author | : Rashid Pierre Riboul |
Publisher | : |
Total Pages | : 58 |
Release | : 2019 |
ISBN-10 | : OCLC:1112495731 |
ISBN-13 | : |
Rating | : 4/5 (31 Downloads) |
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Author | : Rashid Pierre Riboul |
Publisher | : |
Total Pages | : 58 |
Release | : 2019 |
ISBN-10 | : OCLC:1112495731 |
ISBN-13 | : |
Rating | : 4/5 (31 Downloads) |
Author | : Douglas T. Gjerde |
Publisher | : John Wiley & Sons |
Total Pages | : 206 |
Release | : 2009-07-10 |
ISBN-10 | : 9783527627202 |
ISBN-13 | : 3527627200 |
Rating | : 4/5 (02 Downloads) |
This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis. By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation.
Author | : |
Publisher | : |
Total Pages | : 1780 |
Release | : 2004 |
ISBN-10 | : MINN:31951P00992543M |
ISBN-13 | : |
Rating | : 4/5 (3M Downloads) |
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Author | : |
Publisher | : Academic Press |
Total Pages | : 675 |
Release | : 2015-06-06 |
ISBN-10 | : 9780128019368 |
ISBN-13 | : 0128019360 |
Rating | : 4/5 (68 Downloads) |
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs. - Continues the legacy of this premier serial with quality chapters on structures of large RNA molecules and their complexes
Author | : Roland K. Hartmann |
Publisher | : John Wiley & Sons |
Total Pages | : 1368 |
Release | : 2015-03-31 |
ISBN-10 | : 9783527650552 |
ISBN-13 | : 3527650555 |
Rating | : 4/5 (52 Downloads) |
The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.
Author | : Frank J. Schmidt |
Publisher | : Humana Press |
Total Pages | : 0 |
Release | : 2014-10-29 |
ISBN-10 | : 1493918958 |
ISBN-13 | : 9781493918959 |
Rating | : 4/5 (58 Downloads) |
In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.
Author | : Roland K. Hartmann |
Publisher | : John Wiley & Sons |
Total Pages | : 1370 |
Release | : 2015-06-22 |
ISBN-10 | : 9783527327768 |
ISBN-13 | : 3527327762 |
Rating | : 4/5 (68 Downloads) |
The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.
Author | : Christina Waldsich |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2016-08-23 |
ISBN-10 | : 149396058X |
ISBN-13 | : 9781493960583 |
Rating | : 4/5 (8X Downloads) |
Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.
Author | : David Elliott |
Publisher | : Oxford University Press |
Total Pages | : 445 |
Release | : 2017-01-31 |
ISBN-10 | : 9780191071034 |
ISBN-13 | : 019107103X |
Rating | : 4/5 (34 Downloads) |
RNA plays a central, and until recently, somewhat underestimated role in the genetics underlying all forms of life on earth. This versatile molecule not only plays a crucial part in the synthesis of proteins from a DNA template, but is also intrinsically involved in the regulation of gene expression, and can even act as a catalyst in the form of a ribozyme. This latter property has led to the hypothesis that RNA - rather than DNA - could have played an essential part in the origin of life itself. This landmark text provides a systematic overview of the exciting and rapidly moving field of RNA biology. Key pioneering experiments, which provided the underlying evidence for what we now know, are described throughout, while the relevance of the subject to human disease is highlighted via frequent boxes. For the second edition of Molecular Biology of RNA, more introductory material has been incorporated at the beginning of the text, to aid students studying the subject for the first time. Throughout the text, new material has been included - particularly in relation to RNA binding domains, non-coding RNAs, and the connection between RNA biology and epigenetics. Finally, a new closing chapter discusses how exciting new technologies are being used to explore current topical areas of research.
Author | : I.M. Rosenberg |
Publisher | : Springer Science & Business Media |
Total Pages | : 453 |
Release | : 2013-03-14 |
ISBN-10 | : 9781475711080 |
ISBN-13 | : 1475711085 |
Rating | : 4/5 (80 Downloads) |
This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip ment other than encouragement to read the manual, if you can find it. Through out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.