Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular BiologyTM series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.

This unique and practical book provides quick and easy access to data on the physical and chemical properties of all classes of materials. The second edition has been much expanded to include whole new families of materials while many of the existing families are broadened and refined with new material and up-to-date information. Particular emphasis is placed on the properties of common industrial materials in each class. Detailed appendices provide additional information, and careful indexing and a tabular format make the data quickly accessible. This book is an essential tool for any practitioner or academic working in materials or in engineering.

This is a comprehensive analysis of ethical topics in palliative care, combining clinical experience and philosophical rigor. A broad array of topics are explored from historical, legal, clinical, and ethical perspectives, offering both the seasoned clinician and interested lay reader a thorough examination of the complex ethical issues facing patients suffering from life-threatening illness.

Ultraviolent (UV) radiation represents a comparatively small part of the total electromagnetic spectrum, yet this portion of the spectrum is responsible for an extremely wide range of effects in many different settings. In biological systems, it can produce good and bad outcomes. We use UV radiation to drive chemical reactions that are desired, and we know that it also drives environmental reactions that are harmful to living organisms. We use UV radiation to interrogate molecules and to analyze cells. Some animals view our world in the UV radiation range, while astronomers use UV to study the universe; in both cases, what is observed is very different from that observed using other parts of the electromagnetic spectrum. This volume gives the reader just the very beginning of the broad range of properties, applications, and effects of UV radiation and is intended to stimulate the reader to explore more of this fascinating part of the electromagnetic spectrum.