Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics. This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results. - Includes full listings and discussions of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting across 100 protocols - Features clear, step-by-step instruction with color diagrams and images, followed by worked examples of putting lab techniques into action - Empowers researchers and clinicians to reproduce research and clinical methods and ensure test accuracy

This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.

Now fully revised and updated, Clinical Biochemistry, third edition is essential reading for specialty trainees, particularly those preparing for postgraduate examinations. It is also an invaluable current reference for all established practitioners, including both medical and scientist clinical biochemists. Building on the success of previous editions, this leading textbook primarily focuses on clinical aspects of the subject, giving detailed coverage of all conditions where clinical biochemistry is used in diagnosis and management - including nutritional disorders, diabetes, inherited metabolic disease, metabolic bone disease, renal calculi and dyslipidaemias. The acquisition and interpretation of clinical biochemical data are also discussed in detail. Expanded sections on haematology and immunology for clinical biochemists provide a thorough understanding of both laboratory and clinical aspects New chapters are included on important evolving areas such as the metabolic response to stress, forensic aspects of clinical biochemistry and data quality management An extended editorial team - including three expert new additions - ensures accuracy of information and relevance to current curricula and clinical practice A superb new accompanying electronic version provides an enhanced learning experience and rapid reference anytime, anywhere! Elsevier ExpertConsult.com Enhanced eBooks for medical professionals Compatible with PC, Mac®, most mobile devices and eReaders, browse, search, and interact with this title - online and offline. Redeem your PIN at expertconsult.com today! Straightforward navigation and search across all Elsevier titles Seamless, real-time integration between devices Adjustable text size and brightness Notes and highlights sharing with other users through social media Interactive content

Clinical biochemistry is an analytical and interpretative science. The analytical part involves the determination of the level of chemical components in body fluids and tissues. The interpretative part examines these results and uses them in the diagnosis of disease, the screening for susceptibility to specific diseases, and the monitoring of the progress of treatment. This book is designed to cover the major techniques and analytical instruments used in clinical biochemistry.Each chapter of this book is based on a specific technique, or techniques, with associated instrumentation. These are discussed in some detail. A historical introduction is included for most of the techniques, and the current uses of the techniques are presented. Following that is a series of practical exercises. The first exercises in most of the chapters are a general introduction to the technique, leading to those with a clinical bias. Where applicable, the clinical practical exercises are associated with a case history and/or the discussion of the relevance of the assay to diagnosis and prognosis and to the monitoring of recovery. Each chapter concludes with a selection of appropriate references.

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Clinical Biochemistry Lecture Notes presents the fundamental science behind common biochemical investigations used in clinical practice. Taking a system-based approach, it explores the underlying physiological rationale for tests, with each test explained within the context of disruption by disease. It also explores the value and limitations of biochemical investigations, while helping readers to quickly develop the knowledge and skills required to select the appropriate investigations for diagnosis and management, and to correctly interpret test results. Case studies throughout chapters place the information within a clinical context to further assist readers in the development of test-selection and interpretation skills. Key features include: A comprehensive, yet concise overview of the science behind common biochemical investigations Helps readers rapidly acquire a fully integrated, practical understanding of biochemical diagnostics Full-colour flowcharts and algorithms detailing the rationale for tests, the biochemical processes involved, and test procedures, for quick comprehension and reference More clinical cases demonstrating application to practice Now in its tenth edition, this classic introductory, reference, and revision text is indispensable to medical students, and all those who want to quickly acquire a practical understanding of the scientific principles underpinning biochemical tests and a working knowledge of test selection, test procedures, and the interpretation of results within a clinical context.

Techniques in Clinical Chemistry: A Handbook for Medical Laboratory Technicians is a five-chapter supplementary handbook on the fundamentals of medical laboratory test and protocols. The opening chapter covers the fundamental aspects of medical laboratories, including accuracy measures, methods of analysis, buffers, indicators, and valency. These topics are followed by discussions on the procedures for preliminary preparation, primarily for sample preparation and specimen collection. Considerable chapters are devoted to the scrutinized analysis of specific specimen, such as blood, cerebrospinal fluid, feces, gastric sample, and urine. The final chapter discusses technical essentials of renal and hepatic function tests. This handbook is directed toward medical and laboratory technicians and clinicians.

This book provides detailed information on various instruments, techniques and experiment protocols of biochemistry and molecular biology. It deals with basic as well as advanced information and in-depth methodology in simple language to help students and professionals to perform experiments with ease. This book not only clears the practical concepts of Biochemistry and Molecular Biology at undergraduate and post-graduation levels, but also helps to pass the Ph.D. course work exam conducted by various universities. This book will develop research aptitude to clear the NET examination. This manual gives a comprehensive idea about the various instruments, their working, troubleshooting and their applications. It provides a wide spectrum of 14 chapters covering basic as well as advanced techniques and instrumentation, viz., Gas Chromatography (GC), Mass Spectrometry (MS), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) with detailed protocols. Most of the experiments can be easily performed in the laboratory having basic facilities. Historical background, experiment nature, its principle, step-by-step procedure with diagrammatic representation and important precautions are given in the beginning of each experiment.

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.