Pulse EPR (electron paramagnetic resonance) is one of the newest and most widely used techniques for examining the structure, function and dynamics of biological systems and synthetic materials. Until now, however, there has been no single text dedicated to this growing area of research. This text addresses the need for a comprehensive overview of Pulse EPR. The book covers the basic theory of pulse EPR, as well as a description and critical evaluation of the existing and emerging methods needed for selecting and conducting the proper experiment and analyzing the results. This is an indispensable reference for all scientists who need a thorough grounding in this increasingly popular field of spectroscopy.

This unique, self-contained resource is the first volume on electron paramagnetic resonance (EPR) spectroscopy in the eMagRes Handbook series. The 27 chapters cover the theoretical principles, the common experimental techniques, and many important application areas of modern EPR spectroscopy. EPR Spectroscopy: Fundamentals and Methods is presented in four major parts: A: Fundamental Theory, B: Basic Techniques and Instrumentation, C: High-Resolution Pulse Techniques, and D: Special Techniques. The first part of the book gives the reader an introduction to basic continuous-wave (CW) EPR and an overview of the different magnetic interactions that can be determined by EPR spectroscopy, their associated theoretical description, and their information content. The second provides the basics of the various EPR techniques, including pulse EPR, and EPR imaging, along with the associated instrumentation. Parts C and D builds on parts A and B and offer introductory accounts of a wide range of modern advanced EPR techniques, with examples of applications. The last two parts presents most of the new advances that do not appear in most of the classical EPR textbooks that focus on CW EPR. EPR Spectroscopy: Fundamentals and Methods contains, in concise form, all the material needed to understand state-of-the-art EPR spectroscopy at the graduate school/research level, whilst the editors have ensured that it presents the topic at a level accessible to newcomers to the field and others who want to know its range of application and how to apply it.

This book offers a pragmatic guide to navigating through the complex maze of EPR/ESR spectroscopy fundamentals, techniques, and applications. Written for the scientist who is new to EPR spectroscopy, the editors have prepared a volume that de-mystifies the basic fundamentals without weighting readers down with detailed physics and mathematics, and then presents clear approaches in specific application areas. The first part presents basic fundamentals and advantages of electron paramagnetic resonance spectrscopy. The second part explores severalapplication areas including chemistry, biology, medicine, materials and geology. A frequently-asked-questions sections focuses on practicalquestions, such as the size of sample, etc. It's an ideal, hands-on reference for chemists and researchers in the pharmaceutical and materials (semiconductor) industries who are looking for a basic introduction to EPR spectroscopy.

Although originally invented and employed by physicists, electron paramagnetic resonance (EPR) spectroscopy has proven to be a very efficient technique for studying a wide range of phenomena in many fields, such as chemistry, biochemistry, geology, archaeology, medicine, biotechnology, and environmental sciences. Acknowledging that not all studies require the same level of understanding of this technique, this book thus provides a practical treatise clearly oriented toward applications, which should be useful to students and researchers of various levels and disciplines. In this book, the principles of continuous wave EPR spectroscopy are progressively, but rigorously, introduced, with emphasis on interpretation of the collected spectra. Each chapter is followed by a section highlighting important points for applications, together with exercises solved at the end of the book. A glossary defines the main terms used in the book, and particular topics, whose knowledge is not required for understanding the main text, are developed in appendices for more inquisitive readers.

The first volume devoted entirely to Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy This valuable book provides an introduction and broad survey of topics in ESEEM spectroscopy, including the theory, instrumentation, peculiarities of ESE experiments, and analysis of experimental data with particular emphasis on orientationally disordered systems. Applications of ESEEM spectroscopy to study chemically and biologically important paramagnetic centers in single crystals, amorphous solids, and powders are discussed as well. Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy will benefit specialists in magnetic resonance spectroscopy, physicists, chemists, and biologists who use magnetic resonance in their research.

Spin Resonance Spectroscopy: Principles and Applications presents the principles, recent advancements and applications of nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) in a single multi-disciplinary reference. Spin resonance spectroscopic techniques through NMR and EPR are widely used by chemists, physicists, biologists and medicinal chemists. This book addresses the need for new spin resonance spectroscopy content while also presenting the principles, recent advancements and applications of NMR and EPR simultaneously. Ideal for researchers and students alike, the book provides a single source of NMR and EPR applications using a dynamic, holistic and multi-disciplinary approach. - Presents a highly interdisciplinary approach by including NMR and EPR applications in chemistry, physics, biology and biotechnology - Addresses both NMR and EPR, making its concepts and applications implementable in multiple resonance environments and core scientific disciplines - Features a broad range of methods, examples and illustrations for both NMR and EPR to aid in retention and underscore key concepts

Spin-label electron paramagnetic resonance (EPR) spectroscopy is a versatile molecular probe method that finds wide application in molecular biophysics and structural biology. This book provides the first comprehensive summary of basic principles, spectroscopic properties, and use for studying biological membranes, protein folding, supramolecular structure, lipid-protein interactions, and dynamics. The contents begin with discussion of fundamental theory and practice, including static spectral parameters and conventional continuous-wave (CW) spectroscopy. The development then progresses, via nonlinear CW-EPR for slower motions, to the more demanding time-resolved pulse EPR, and includes an in-depth treatment of spin relaxation and spectral line shapes. Once the spectroscopic fundamentals are established, the final chapters acquire a more applied character. Extensive appendices at the end of the book provide detailed summaries of key concepts in magnetic resonance and chemical physics for the student reader and experienced practitioner alike. Key Features: Indispensable reference source for the understanding and interpretation of spin-label spectroscopic data in its different aspects. Tables of fundamental spectral parameters are included throughout. Forms the basis for an EPR graduate course, extending up to a thorough coverage of advanced topics in Specialist Appendices. Includes all necessary theoretical background. The primary audience is research workers in the fields of molecular biophysics, structural biology, biophysical chemistry, physical biochemistry and molecular biomedicine. Also, physical chemists, polymer physicists, and liquid-crystal researchers will benefit from this book, although illustrative examples used are often taken from the biomolecular field. Readers will be postgraduate researchers and above, but include those from other disciplines who seek to understand the primary spin-label EPR literature.

SPECTROSCOPY FOR MATERIALS CHARACTERIZATION Learn foundational and advanced spectroscopy techniques from leading researchers in physics, chemistry, surface science, and nanoscience In Spectroscopy for Materials Characterization, accomplished researcher Simonpietro Agnello delivers a practical and accessible compilation of various spectroscopy techniques taught and used to today. The book offers a wide-ranging approach taught by leading researchers working in physics, chemistry, surface science, and nanoscience. It is ideal for both new students and advanced researchers studying and working with spectroscopy. Topics such as confocal and two photon spectroscopy, as well as infrared absorption and Raman and micro-Raman spectroscopy, are discussed, as are thermally stimulated luminescence and spectroscopic studies of radiation effects on optical materials. Each chapter includes a basic introduction to the theory necessary to understand a specific technique, details about the characteristic instrumental features and apparatuses used, including tips for the appropriate arrangement of a typical experiment, and a reproducible case study that shows the discussed techniques used in a real laboratory. Readers will benefit from the inclusion of: Complete and practical case studies at the conclusion of each chapter to highlight the concepts and techniques discussed in the material Citations of additional resources ideal for further study A thorough introduction to the basic aspects of radiation matter interaction in the visible-ultraviolet range and the fundamentals of absorption and emission A rigorous exploration of time resolved spectroscopy at the nanosecond and femtosecond intervals Perfect for Master and Ph.D. students and researchers in physics, chemistry, engineering, and biology, Spectroscopy for Materials Characterization will also earn a place in the libraries of materials science researchers and students seeking a one-stop reference to basic and advanced spectroscopy techniques.

Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.

This unique, self-contained resource is the first volume on electron paramagnetic resonance (EPR) spectroscopy in the eMagRes Handbook series. The 27 chapters cover the theoretical principles, the common experimental techniques, and many important application areas of modern EPR spectroscopy. EPR Spectroscopy: Fundamentals and Methods is presented in four major parts: A: Fundamental Theory, B: Basic Techniques and Instrumentation, C: High-Resolution Pulse Techniques, and D: Special Techniques. The first part of the book gives the reader an introduction to basic continuous-wave (CW) EPR and an overview of the different magnetic interactions that can be determined by EPR spectroscopy, their associated theoretical description, and their information content. The second provides the basics of the various EPR techniques, including pulse EPR, and EPR imaging, along with the associated instrumentation. Parts C and D builds on parts A and B and offer introductory accounts of a wide range of modern advanced EPR techniques, with examples of applications. The last two parts presents most of the new advances that do not appear in most of the classical EPR textbooks that focus on CW EPR. EPR Spectroscopy: Fundamentals and Methods contains, in concise form, all the material needed to understand state-of-the-art EPR spectroscopy at the graduate school/research level, whilst the editors have ensured that it presents the topic at a level accessible to newcomers to the field and others who want to know its range of application and how to apply it.