Recently there have been major developments in the experimental techniques available for the study of the primary events following the absorption of ultra-violet and visible radiation by biological systems. These techniques, which include absorption, emission, resonance Raman, electron spin resonance, nuclear magnetic resonance and photoacoustic spectroscopies, can be used to study the fate of transient species with lifetimes ranging from seconds to nanoseconds and extending in some cases, such as laser flash photolysis, to pico 12 15 (10- S)- and even femtoseconds (10- s). In parallel with these developments there has been a dramatic increase in the use of light in medicine via the direct photochemical alteration of endogenous molecules (phototherapy) or via the photoactivation of drugs in the skin or other tissue (photochemotherapy). Thus neonatal hyperbili rubinaemia can be routinely treated by phototherapy and psoriasis is frequently treated by PUVA photochemotherapy. A promising new photo chemotherapy used the phototoxicity of porphyrin drugs activated by red light to destroy solid malignant tumors. While some of the overall qualitative effects of such treatments are known, only recently have we begun to understand the associated molecular mechanisms. The primary molecular processes involve short-lived species. The pur pose of this Advanced Study Institute was to review some newer experi mental techniques for the study of such species, the application of these techniques to biological and medical systems and to examine the value of such information in phototherapeutic situations.

First multi-year cumulation covers six years: 1965-70.

The first edition of The Science of Photobiology was published in 1977, and was the first textbook to cover all of the major areas of photobiology. The science of photobiology is currently divided into 14 subspecialty areas by the American Society for Photobiology. In this edition, however, the topics of phototechnology and spectroscopy have been com bined in a new chapter entitled "Photophysics." The other subspecialty areas remain the same, i.e., Photochemistry, Photosensitization, UV Radiation Effects, Environmental Photobiology, Photomedicine, Circadian Rhythms, Extraretinal Photoreception, Vision, Photomorphogenesis, Photomovement, Photosynthesis, and Bioluminescence. This book has been written as a textbook to introduce the science of photobiology to advanced undergraduate and graduate students. The chapters are written to provide a broad overview of each topic. They are designed to contain the amount of information that might be presented in a one-to two-hour general lecture. The references are not meant to be exhaustive, but key references are included to give students an entry into the literature. Frequently a more recent reference that reviews the literature will be cited rather than the first paper by the author making the original discovery. The chapters are not meant to be a repository of facts for research workers in the field, but rather are concerned with demon strating the importance of each specialty area of photobiology, and documenting its relevance to current and/or future problems of man.

Almost all life depends on light for its survival. It is the ultimate basis for the food we eat (photosynthesis), and many organisms make use of it in basic sensory mechanisms for guiding their behaviour, be it through the complex process of vision, or by the relatively more simple photosens itivity of microorganis~urthermore, light has profound implications for the field of medicine, both as a cause of disease (ie UV damage of DNA), and as a therapeutic agent (ie photodynamic therapy). These and other processes are the basis for the science of photobiolog~ which could be defined as the study of the effects of (visible and ultraviolet) light (from both the sun and artificial sources) on living matter. By its very nature, therefore, it is a multidisciplinary science involving branches of biology, chemistry, physics and medicine. This book contains a selection of papers which have been chosen to highlight recent advances in the various disciplines that make up photo biology. Although no book on photobiology can hope to be comprehensive, we hope that this volume includes a representative sample of much of what is new in the field. It is, however, inevitable that some areas will be better represented than others reflecting the biases of conference org anisers and editors.

First multi-year cumulation covers six years: 1965-70.

Radiation induces a variety of chemical processes in biological tissues. This volume is a synthesis of up-to-the-minute reviews on such photochemical and photobiological sensitized reactions with particular relevance to photomedicine. The first part gives a description of experimental techniques for the study of the primary processes after radiation absorption by biological systems. It is followed by chapters on singlet oxygen and photomedicine, considering both phototherapy and photochemotherapy. These sections also discuss the next generation of potential photosensitizing drugs.

In more than 20 contributions new trends in cancer therapy are reviewed and critically discussed by highly competent authors. Their contributions are based on an international symposium and cover all major fields of clinical oncology. Special emphasis is given to surgical oncology (including photodynamic laser therapy and radiosurgery), radiation oncology, hyperthermia, new antineoplastic agents, enzyme-pattern-targeted chemotherapy, drug resistance, biological response modifiers, bone marrow transplantation, antibody guided therapy, induction of cell differentiation in transformed cells, and the individualization of tumor therapy. The stimulating discussion at the end of each section covering these topics will particulary benefit the clinical oncologist and all researchers devoted to the improvement of cancer therapy.

This volume contains the Proceedings of a two-week NATO Advanced Study Institute on "Laser Systems for Photobiology and Photomedicine", conducted from May 11 to 20, 1990 in Erice, Italy. This is the 15th annual course of the International School of Quantum Electronics (ISQE), organized under the auspices of the "Ettore Majorana" Center for Scientific Culture. The application of lasers to medicine and surgery has made amazing progress since the last ISQE Course on this subject in 1983. The present Proceedings give a tutorial introduction to today's most important areas, as well as a review of current results by leading researchers. Among the possible approaches to a NATO Advanced Study Institute on Laser Systems for Photobiology and Photomedicine, we chose to emphasize the scientific and technological aspects of advanced laser systems when applied to laboratory and clinical tests. Since it is the policy of the School to stress the advanced scientific and techn·ological achievements in the field of Quantum Electronics, the Course broadly covers performance already achieved and potential applications.

Providing the guidance needed for formulation, handling, and quality control of photolabile drugs, Photostability of Drugs and Drug Formulations, Second Edition explores the significance of new information on drug photoreactivity in a pharmaceutical context. Completely revised and updated, with chapter authors drawn from an international panel of experts, the book supplies the background necessary for planning standardized photochemical stability studies as a part of drug development and formulation work. It contains comprehensive coverage of the physical and chemical aspects of drug photoreactivity, formulation, stability testing, and drug design/discovery in one resource. The contents have been reorganized to focus on the standardization of photostability testing of drug substances and products, in vitro photoreactivity screening of drugs, and various aspects of the formulation of photoreactive substances. The information on in vitro screening of drug photoreactivity is of great relevance for scientists who are developing and validating a set of testing protocols to address photosafety. Discussing kinetic and chemical aspects of drug photodecomposition as well as the practical problems frequently encountered in photochemical stability testing, this book helps you design a test protocol and interpret the results. Features Assists non-experts in this field design a test protocol and interpret the results Covers in vitro and in vivo aspects of interactions between drugs and light Explores the kinetic and chemical aspects of drug photodecomposition Discusses the problems frequently encountered in photochemical stability testing Provides guidance on how to address photosafety assessments and labeling requirements of potentially photoreactive drugs Highlights the practical implications of drug photodecomposition from a pharmaceutical viewpoint Offers specific guidance in photostability testing and screening of drug photoreactivity