Practical Methods In Molecular Biology
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Author |
: Robert F. Schleif |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 229 |
Release |
: 2012-12-06 |
ISBN-10 |
: 9781461259565 |
ISBN-13 |
: 1461259568 |
Rating |
: 4/5 (65 Downloads) |
Synopsis Practical Methods in Molecular Biology by : Robert F. Schleif
This volume has evolved from a laboratory methods book that one of us first compiled nearly fifteen years ago. Since that time the book has undergone many minor revisions in order to include new methods and updated versions of older methods. The result has been an increasingly useful and more widely circulated book. However, the recent series of technological explosions generally lumped together under the name of "recombinant DNA technology" has been a turning point in the evolution of this previously underground publication. Minor revisions will no longer do. To keep the book useful we have had to make major revi sions and additions. The result is a dramatically expanded book that should be more useful to more people. The larger size and wider usefulness of the book have made this more formal publication seem a reasonable step to take. One of the reasons that this volume should be useful to many people is that it includes only procedures that have been used repeatedly by us and that have proven highly reliable both to ourselves and to others in our laboratories.
Author |
: Khalid Z. Masoodi |
Publisher |
: Academic Press |
Total Pages |
: 220 |
Release |
: 2020-10-28 |
ISBN-10 |
: 9780128244500 |
ISBN-13 |
: 012824450X |
Rating |
: 4/5 (00 Downloads) |
Synopsis Advanced Methods in Molecular Biology and Biotechnology by : Khalid Z. Masoodi
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author |
: Heather B. Miller |
Publisher |
: Academic Press |
Total Pages |
: 227 |
Release |
: 2011-10-18 |
ISBN-10 |
: 9780123855459 |
ISBN-13 |
: 0123855454 |
Rating |
: 4/5 (59 Downloads) |
Synopsis Molecular Biology Techniques by : Heather B. Miller
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author |
: Bal Ram Singh |
Publisher |
: Cambridge University Press |
Total Pages |
: 378 |
Release |
: 2022-06-16 |
ISBN-10 |
: 9781108486408 |
ISBN-13 |
: 1108486401 |
Rating |
: 4/5 (08 Downloads) |
Synopsis Practical Techniques in Molecular Biotechnology by : Bal Ram Singh
The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.
Author |
: Robert F Schleif |
Publisher |
: |
Total Pages |
: 236 |
Release |
: 1981-11-30 |
ISBN-10 |
: 1461259576 |
ISBN-13 |
: 9781461259572 |
Rating |
: 4/5 (76 Downloads) |
Synopsis Practical Methods in Molecular Biology by : Robert F Schleif
Author |
: Keith Wilson |
Publisher |
: Cambridge University Press |
Total Pages |
: 769 |
Release |
: 2010-03-04 |
ISBN-10 |
: 9780521516358 |
ISBN-13 |
: 0521516358 |
Rating |
: 4/5 (58 Downloads) |
Synopsis Principles and Techniques of Biochemistry and Molecular Biology by : Keith Wilson
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Author |
: Stefan Surzycki |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 442 |
Release |
: 2012-12-06 |
ISBN-10 |
: 9783642569685 |
ISBN-13 |
: 3642569684 |
Rating |
: 4/5 (85 Downloads) |
Synopsis Basic Techniques in Molecular Biology by : Stefan Surzycki
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author |
: J.M. Walker |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 304 |
Release |
: 2012-12-06 |
ISBN-10 |
: 9789401165631 |
ISBN-13 |
: 9401165637 |
Rating |
: 4/5 (31 Downloads) |
Synopsis Techniques in Molecular Biology by : J.M. Walker
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Author |
: Terence Austen Brown |
Publisher |
: Oxford University Press, USA |
Total Pages |
: 289 |
Release |
: 2001 |
ISBN-10 |
: 0199636451 |
ISBN-13 |
: 9780199636457 |
Rating |
: 4/5 (51 Downloads) |
Synopsis Essential Molecular Biology by : Terence Austen Brown
The objective of these volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. The second volume details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression).
Author |
: Brenda D. Spangler |
Publisher |
: Wiley |
Total Pages |
: 232 |
Release |
: 2002-06-14 |
ISBN-10 |
: 0470843608 |
ISBN-13 |
: 9780470843604 |
Rating |
: 4/5 (08 Downloads) |
Synopsis Methods in Molecular Biology and Protein Chemistry by : Brenda D. Spangler
Ein praxisorientierter Leitfaden für Studenten im Hauptstudium. Vermittelt werden aktuelle Techniken der Molekularbiologie und Biochemie als fester Bestandteil eines Forschungsprojektes. Auf diese Weise kann der Lernende die Auswirkung der Gentechnik anhand der praktischen Anwendung von nassanalytischen biochemischen Verfahren erkennen. Mit Computer-Tutorials für Sequenzierung, Genomik und Molekülmodellierung. Mit Tipps und Hinweisen zur Sichtung der Primärliteratur, zu mündlichen Berichten, zur Protokollierung des Labortagebuchs und zum Verfassen von Berichten. Mit einer Liste von amerikanischen und europäischen kommerziellen Anbietern von Reagenzien. Verständlich und anschaulich geschrieben. Ein unverzichtbares Lehrbuch für fortgeschrittene Studenten und Doktoranden. "Methods in Molecular Biology" ist jedoch NICHT geeignet für aktive Forscher auf der Suche nach den neuesten hochdifferenzierten Verfahren.