Describing recent developments in the engineering and generation of plants as production platforms for biopharmaceuticals, this book includes both vaccines and monoclonal antibodies. It has a particular emphasis on targeting diseases which predominate in less developed countries, encompassing the current state of technologies and describing expression systems and applications. This book also includes a variety of vaccine case studies, protecting against pervasive infectious diseases such as rabies, influenza and HIV.

The enormous progress made in plant genomics is opening the possibility of using plants as bioreactors for the production of pharmaceutical products, ranging from immunogens (single peptides to complex structures such as virus-like particles) to adjuvants, microbicides and monoclonal antibodies. This book includes 11 chapters spanning most of the issues involved in plant-based vaccine production, including intellectual property considerations and veterinary applications. In addition to the detailed description of antigen expression in different plant systems, adjuvant production and plant glyc.

The book traces the roots of plant biotechnology from the basic sciences to current applications in the biological and agricultural sciences, industry, and medicine. Providing intriguing opportunities to manipulate plant genetic and metabolic systems, plant biotechnology has now become an exciting area of research. The book vividly describes the processes and methods used to genetically engineer plants for agricultural, environmental and industrial purposes, while also discussing related bioethical and biosafety issues. It also highlights important factors that are often overlooked by methodologies used to develop plants’ tolerance against biotic and abiotic stresses and in the development of special foods, bio-chemicals, and pharmaceuticals. The topics discussed will be of considerable interest to both graduate and postgraduate students. Further, the book offers an ideal reference guide for teachers and researcher alike, bridging the gap between fundamental and advanced approaches.

Immunopotentiators in Modern Vaccines provides an in-depth insight and overview of a number of most promising immunopotentiators in modern vaccines. In contrast to existing books on the subject it provides recent data on the critical mechanisms governing the activity of vaccine adjuvants and delivery systems. Knowledge of immunological pathways and scenarios of the cells and molecules involved is described and depicted in comprehensive illustrations. - Contributions from leading international authorities in the field - Well-illustrated, informative figures present the interactions between immunopotentiators and the host immune system - Each chapter lists advantages and potential hurdles for achieving a practical application for the specific immunopentiator

This comprehensive, authoritative treatise covers all aspects of mucosal vaccines including their development, mechanisms of action, molecular/cellular aspects, and practical applications. The contributing authors and editors of this one-of-a-kind book are very well known in their respective fields. Mucosal Vaccines is organized in a unique format in which basic, clinical, and practical aspects of the mucosal immune system for vaccine development are described and discussed. This project is endorsed by the Society for Mucosal Immunology. - Provides the latest views on mucosal vaccines - Applies basic principles to the development of new vaccines - Links basic, clinical, and practical aspects of mucosal vaccines to different infectious diseases - Unique and user-friendly organization

Biomedical Innovations to Combat COVID-19 provides an updated overview on the development of vaccines, antiviral drugs and nanomaterials, and diagnostic methods for the fight against COVID-19. Perspectives on such technologies are identified, discussed, and enriched with figures for easy understanding and applicability. Furthermore, it contains basic aspects of virology, immunology, and antiviral drugs that are needed to fully appreciate these innovations. This book is split into four sections: introduction, presenting basic virologic and epidemiological aspects of COVID-19; vaccines against COVID-19, discussing their different types and applications used to develop them; diagnostic approaches for SARS-CoV-2, encompassing advanced sensing and microfluidic-based biosensors; and drug development and delivery, where antivirals based on nanomaterials or drugs are presented. It is a valuable source for virologists, biotechnologists, and members of biomedical field interested in learning more about how novel technologies can be applied to fasten the eradication of the COVID-19 and similar pandemics. - Presents updated literature coverage summarizing the most relevant information on COVID-19 - Written by experts from diverse scientific domains in order to provide readers with a thorough view on the subject - Encompasses tables, figures and information trees especially developed for the book in order to condense and highlight key points for quick reference

Preventable bacterial infections continue to be a major hazard to human health in an age which has experienced a proliferation in readily available vaccines for such diseases. Molecular and Clinical Aspects of Bacterial Vaccine Development examines the latest developments in bacterial vaccinology and the impact they have had on the control of bacterial infections by comprehensively reviewing old, current and future vaccines. Ten of the most globally prevalent bacterial pathogens are covered, providing an indepth analysis of novel strategies for vaccine design and of the state-of-the-art technology available in this field. This timely and authoritative review of vaccine development will be an essential source of information for scientists, clinicians and public health specialists, as well as postgraduate students in relevant fields.

Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases: An Integrated View provides an integrated outlook of the disciplines involved in the development of plant-based vaccines as well as an updated compilation of the successful developments in the field. The volume covers immunological aspects of mucosal vaccine design, molecular approaches to attain high levels of the recombinant antigens, the rationale of using bioreactor to expand plant biomass, and pharmaceutical technology approaches that have been applied to the development of plant-based vaccine formulations. Practical figures and tables are presented to facilitate reading and identification of key points. Perspectives for this field are also discussed. Written by authorities in the field, Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases: An Integrated View is a comprehensive resource for researchers and students interested in plant genetics and breeding, immunology, and genetic engineering.

This dissertation, "Production of Transgenic Plant-derived Vaccines via Plastid Transformation Technology" by Yuk-ting, Lee, 李玉婷, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled PRODUCTION OF TRANSGENIC PLANT-DERIVED VACCINES VIA PLASTID TRANSFORMATION TECHNOLOGY Submitted by Lee Yuk Ting for the degree of Master of Philosophy at The University of Hong Kong in July 2004 With the advent of genetic engineering in higher plants, the need for very large quantities of therapeutic protein at low cost, and the desire to have heat-stable edible vaccines directed at human and animal diseases, transgenic plant-derived vaccines offer a new strategy for the development of safe, inexpensive vaccines against infectious diseases. The first success of plastid transformation in tobacco in 1990 has opened up the opportunities for genetically modifying plastids in higher plants for high level expression of biopharmaceuticals, such as antibodies and vaccines for oral administration. Since each plant cell contains up to 10,000 copies of identical plastid genome, plastid engineering should result in very high levels of transgene expression. In addition, due to the prokaryotic feature of the plastids, engineering the plastid genome bears the advantages of expressing multiple genes as operons without the problem of positional and epigenetic effects affecting transgene activity. Therefore, plastid transformation technology is a promising alternative to the conventional nuclear transformation. The objectives of this research are: 1) to examine if plastids can be used to produce edible vaccines suitable for uses against Epstein-Barr (EBV) and Hepatitis B Virus (HBV), and 2) if such a production can be regulated through a chemically inducible gene expression system. The genes encoding the early antigen of the Epstein-Barr virus and the Hepatitis B surface antigen (HBsAg) are introduced into tobacco plastids via biolistic bombardment techniques. Simultaneously, an alcohol-inducible alc gene expression system is established and integrated into the tobacco nuclear genome for controlling plastid expression of antigen genes. It was found that chloroplast- derived antigen against EBV could be successfully produced in transplastomic tobacco using a constitutive plastid-derived promoter. However, constitutive expression of Hepatitis B surface antigen (HBsAg) apparently results in growth retardation and lethality of the plants. By adapting the ethanol inducible (alc) gene expression system to control engineered plastid operons, it is possible to regulate a marker gene (uidA) expression and the production of antigen (HBsAg) in plastids upon ethanol administration. The technology developed here could contribute to a much more cost-effective system for vaccine production system in the future. DOI: 10.5353/th_b3015943 Subjects: Plastids Transgenic plants Vaccines Viral antigens