A comprehensive presentation of essential topics for biological engineers, focusing on the development and application of dynamic models of biomolecular and cellular phenomena. This book describes the fundamental molecular and cellular events responsible for biological function, develops models to study biomolecular and cellular phenomena, and shows, with examples, how models are applied in the design and interpretation of experiments on biological systems. Integrating molecular cell biology with quantitative engineering analysis and design, it is the first textbook to offer a comprehensive presentation of these essential topics for chemical and biological engineering. The book systematically develops the concepts necessary to understand and study complex biological phenomena, moving from the simplest elements at the smallest scale and progressively adding complexity at the cellular organizational level, focusing on experimental testing of mechanistic hypotheses. After introducing the motivations for formulation of mathematical rate process models in biology, the text goes on to cover such topics as noncovalent binding interactions; quantitative descriptions of the transient, steady state, and equilibrium interactions of proteins and their ligands; enzyme kinetics; gene expression and protein trafficking; network dynamics; quantitative descriptions of growth dynamics; coupled transport and reaction; and discrete stochastic processes. The textbook is intended for advanced undergraduate and graduate courses in chemical engineering and bioengineering, and has been developed by the authors for classes they teach at MIT and the University of Minnesota.

Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Here's a first-of-its-kind book that bridges the gap between biomedical imaging and the bioscience community. This unique resource gives you a detailed understanding of imaging platforms, fluorescence imaging, and fundamental image processing algorithms. Further, it guides you through application of advanced image analysis methods and techniques to specific biological problems. The book presents applications that span a wide range of scales, from the detection of signaling events in sub-cellular structures, to the automated analysis of tissue structures. Other critical areas discussed include the dynamics of cell populations and in vivo microscopy. This cutting-edge volume is supported with over 160 illustrations that support key topics throughout the book. CD-ROM Included! Contains full-color images and videos that further illustrate topics discussed in the book.

Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use

Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.

The chapters in CYTOMETRY MCB volumes, including this 4th Edition, provide comprehensive description of particular cytometric methods and review their applications. Some chapters also describe new instrumentation and provide fundamental information on use of new fluorescent probes and on data analysis. Although the term "edition" suggests the update of earlier volumes, in fact, nearly all chapters of the 4th Edition are devoted to new topics. The authors were invited to present not only technical protocols, such as available in other methodology books that specialize in the protocol format, but also to discuss the aspects of the methodology that generally are not included in the protocols. Many chapters, thus, present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical setting, common traps and pitfalls, problems with data interpretation, comparison with alternative assays, choice of the optimal assay, etc. Some chapters review applications of cytometry and complementary methodologies to particular biological problems or clinical tasks. - Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays - Discusses many new assays developed since the previous edition - Presents recent developments in cytometric intrumentation/technology