Pcr For Clinical Microbiology
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Author |
: Ian W.J. Carter |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 420 |
Release |
: 2010-07-03 |
ISBN-10 |
: 9789048190393 |
ISBN-13 |
: 9048190398 |
Rating |
: 4/5 (93 Downloads) |
Synopsis PCR for Clinical Microbiology by : Ian W.J. Carter
Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology
Author |
: |
Publisher |
: John Wiley & Sons |
Total Pages |
: 2954 |
Release |
: 2020-08-06 |
ISBN-10 |
: 9781555818814 |
ISBN-13 |
: 1555818811 |
Rating |
: 4/5 (14 Downloads) |
Synopsis Clinical Microbiology Procedures Handbook by :
In response to the ever-changing needs and responsibilities of the clinical microbiology field, Clinical Microbiology Procedures Handbook, Fourth Edition has been extensively reviewed and updated to present the most prominent procedures in use today. The Clinical Microbiology Procedures Handbook provides step-by-step protocols and descriptions that allow clinical microbiologists and laboratory staff personnel to confidently and accurately perform all analyses, including appropriate quality control recommendations, from the receipt of the specimen through processing, testing, interpretation, presentation of the final report, and subsequent consultation.
Author |
: U. Reischl |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 253 |
Release |
: 2012-12-06 |
ISBN-10 |
: 9783642483516 |
ISBN-13 |
: 3642483518 |
Rating |
: 4/5 (16 Downloads) |
Synopsis Rapid Cycle Real-Time PCR — Methods and Applications by : U. Reischl
Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.
Author |
: P. Singleton |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 262 |
Release |
: 2013-04-17 |
ISBN-10 |
: 9789401712866 |
ISBN-13 |
: 9401712867 |
Rating |
: 4/5 (66 Downloads) |
Synopsis DNA Methods in Clinical Microbiology by : P. Singleton
DNA Methods in Clinical Microbiology describes the novel DNA-based technology now used in the diagnosis and management of infectious diseases. It is a concise, yet readable, overview written primarily for clinicians, clinical microbiologists, medical students and undergraduates in medical and veterinary microbiology. The book has two primary aims. First, to explain the principles of these methods at the `molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens. Those approaching DNA methods for the first time are assisted by a brief résumé of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index.
Author |
: |
Publisher |
: |
Total Pages |
: |
Release |
: 2007 |
ISBN-10 |
: 1913652351 |
ISBN-13 |
: 9781913652357 |
Rating |
: 4/5 (51 Downloads) |
Synopsis Real-time PCR in Microbiology by :
The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. A recommended book for all microbiology laboratories.
Author |
: Charles W Stratton, MD |
Publisher |
: Demos Medical Publishing |
Total Pages |
: 164 |
Release |
: 2011-07-10 |
ISBN-10 |
: 9781617050480 |
ISBN-13 |
: 1617050482 |
Rating |
: 4/5 (80 Downloads) |
Synopsis Clinical Microbiology by : Charles W Stratton, MD
About the Diagnostic Standards of Care Series A key issue for every laboratory and individual laboratory practitioner is the assessment of risk and a current working knowledge of the standards of care established for diagnostic testing via guidelines, major studies and trials. The Diagnostic Standards of Care series presents an overview of the key diagnoses in clinical pathology using case examples to illustrate effective analysis of the case in light of current evidence and standards for the problem discussed. In addition to being practical diagnostic guides, these volumes will have a unique emphasis on quality assurance and evidence-based testing practices and the role of the pathologist in ensuring quality and patient safety. Clinical Microbiology addresses common medical errors seen in the clinical microbiology laboratory in order to show these errors to pathologists and laboratory technicians as well as clinicians. The goal is to allow such errors to be corrected by both individual effort and a systems approach in the laboratory. The book addresses potential medical errors in test ordering and specimen collection, testing in the laboratory, and reporting and interpretation of test results. Each of these phases can have an adverse impact on the diagnosis and treatment of an infectious disease if a medical error occurs. Potential medical errors are described and discussed in a clinical case-based learning format to effectively illustrate the conditions that contribute to these errors and enable the reader to recognize and avoid them in daily practice. Clinical Microbiology Features Descriptions of potential errors in test ordering and specimen collection in clinical microbiology Descriptions of potential errors in test performance in clinical microbiology Descriptions of potential errors in test reporting and interpretation in clinical microbiology Clinical case discussions provide "real world" illustrations of potential errors and how to anticipate and avoid them in practice Is pocket-sized for portability
Author |
: Dr. Rajdeep Paul |
Publisher |
: DENTOMED PUBLICATION HOUSE |
Total Pages |
: 151 |
Release |
: 2024-06-17 |
ISBN-10 |
: 9788119371808 |
ISBN-13 |
: 8119371801 |
Rating |
: 4/5 (08 Downloads) |
Synopsis Fundamentals of Molecular Diagnostics in Clinical Microbiology by : Dr. Rajdeep Paul
In the ever-evolving landscape of molecular diagnostics, we find ourselves at a unique intersection of science, technology, and human health. This book embarks on an in-depth exploration of the transformative power of molecular diagnostic technologies, which have revolutionized our understanding of microbial pathogens and their impact on global health. From the dawn of molecular biology to the sophisticated diagnostics of today, the journey has been nothing short of extraordinary. Advances in genomic technologies, such as next-generation sequencing and CRISPR-based diagnostics, have not only enhanced our ability to detect and characterize pathogens but have also paved the way for personalized medicine and precision healthcare. These innovations have provided clinicians with unprecedented tools to diagnose, treat, and manage a myriad of infectious diseases with greater accuracy and efficiency. This comprehensive volume is designed to serve as both a foundational text and forward-looking guide for researchers, clinicians, and policymakers involved in the field of molecular diagnostic microbiology. It into the intricate of pathogen detection, the clinical applications of these technologies, and the ethical, legal, and social implications that accompany their use. The chapters ahead will take you through the principles of nucleic acid extraction, the nuances of bioinformatics in diagnostics, and the critical aspects of quality assurance in laboratory settings. You will also discover the emerging trends and future directions in molecular diagnostics, offering a glimpse into the next frontier of microbial exploration. This book is a testament to the collaborative efforts of scientists, healthcare professionals, and regulatory bodies worldwide, who strive to harness the full potential of molecular diagnostics for the betterment of human health. It is our hope that the insights and knowledge contained within these pages will inspire continued innovation and foster a deeper understanding of the vital role that molecular diagnostics play in modern medicine. We invite you to join us on this journey through the fascinating world of molecular diagnostic microbiology, where each discovery brings us closer to a future where the mysteries of infectious diseases are unraveled, and the promise of personalized medicine is fully realized.
Author |
: Mark Wilks |
Publisher |
: Humana Press |
Total Pages |
: 317 |
Release |
: 2016-08-23 |
ISBN-10 |
: 1493960997 |
ISBN-13 |
: 9781493960996 |
Rating |
: 4/5 (97 Downloads) |
Synopsis PCR Detection of Microbial Pathogens by : Mark Wilks
PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one’s own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.
Author |
: Yi-Wei Tang |
Publisher |
: Springer |
Total Pages |
: 760 |
Release |
: 2018-11-09 |
ISBN-10 |
: 9783319951119 |
ISBN-13 |
: 3319951114 |
Rating |
: 4/5 (19 Downloads) |
Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang
In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.
Author |
: Yi-Wei Tang |
Publisher |
: Springer |
Total Pages |
: 544 |
Release |
: 2018-11-09 |
ISBN-10 |
: 9783319339009 |
ISBN-13 |
: 3319339001 |
Rating |
: 4/5 (09 Downloads) |
Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang
In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.