Neurons: Methods and Applications for the Cell Biologist

Neurons: Methods and Applications for the Cell Biologist
Author :
Publisher : Elsevier
Total Pages : 459
Release :
ISBN-10 : 9780080496634
ISBN-13 : 0080496636
Rating : 4/5 (34 Downloads)

Synopsis Neurons: Methods and Applications for the Cell Biologist by :

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. - Contains culture methodology for more than a dozen types of CNS and PNS neurons - Includes most recent and reliable techniques from expert practitioners for specific experimental applications - Addresses the latest strategies for transfecting neurons

Neurons: Methods and Applications for the Cell Biologist

Neurons: Methods and Applications for the Cell Biologist
Author :
Publisher : Academic Press
Total Pages : 500
Release :
ISBN-10 : 0123525667
ISBN-13 : 9780123525666
Rating : 4/5 (67 Downloads)

Synopsis Neurons: Methods and Applications for the Cell Biologist by :

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. Contains culture methodology for more than a dozen types of CNS and PNS neurons Includes most recent and reliable techniques from expert practitioners for specific experimental applications Addresses the latest strategies for transfecting neurons

Neurons: Methods and Applications for the Cell Biologist

Neurons: Methods and Applications for the Cell Biologist
Author :
Publisher : Academic Press
Total Pages : 500
Release :
ISBN-10 : 0123525659
ISBN-13 : 9780123525659
Rating : 4/5 (59 Downloads)

Synopsis Neurons: Methods and Applications for the Cell Biologist by :

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. Contains culture methodology for more than a dozen types of CNS and PNS neurons Includes most recent and reliable techniques from expert practitioners for specific experimental applications Addresses the latest strategies for transfecting neurons

Methods in Cell Biology

Methods in Cell Biology
Author :
Publisher :
Total Pages : 441
Release :
ISBN-10 : OCLC:634876832
ISBN-13 :
Rating : 4/5 (32 Downloads)

Synopsis Methods in Cell Biology by : James R. Baumburg

Cellular and Molecular Methods in Neuroscience Research

Cellular and Molecular Methods in Neuroscience Research
Author :
Publisher : Springer Science & Business Media
Total Pages : 307
Release :
ISBN-10 : 9780387224602
ISBN-13 : 0387224602
Rating : 4/5 (02 Downloads)

Synopsis Cellular and Molecular Methods in Neuroscience Research by : Adalberto Merighi

There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Neuronal Cell Culture

Neuronal Cell Culture
Author :
Publisher : Humana
Total Pages : 205
Release :
ISBN-10 : 1071614398
ISBN-13 : 9781071614396
Rating : 4/5 (98 Downloads)

Synopsis Neuronal Cell Culture by : Shohreh Amini

This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.

Neural Progenitor Cells

Neural Progenitor Cells
Author :
Publisher :
Total Pages : 223
Release :
ISBN-10 : 1071617834
ISBN-13 : 9781071617830
Rating : 4/5 (34 Downloads)

Synopsis Neural Progenitor Cells by : Loic P. Deleyrolle

This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.

The Neuron

The Neuron
Author :
Publisher : Oxford University Press, USA
Total Pages : 570
Release :
ISBN-10 : UOM:39015046931369
ISBN-13 :
Rating : 4/5 (69 Downloads)

Synopsis The Neuron by : Irwin B. Levitan

Intended for use by advanced undergraduate, graduate, and medical students, The Neuron: Cell and Molecular Biology is an intriguing study of the unique biochemical and physiological properties of neurons, which emphasizes the molecular mechanisms that generate and regulate their activity. Keeping abreast of the enormous advances in neuroscience in the five years since the first edition was published, the authors have revised all their chapters in the second edition. What was formerly the first chapter has been expanded substantially and divided into two separate chapters to emphasize the cell biology of neurons and glia, and their commonalities with other kinds of cells. The section on intracellular communication has also been expanded and reorganized. Levitan and Kaczmarek introduce the concept of ion channels as specialized membrane proteins at an early stage, making the idea of selective membrane permeability more accessible in terms of the properties of specific ion channel proteins. In addition, they emphasize the astonishing diversity of voltage-dependent ion channels that has become evident in recent years, and discuss the implications of this diversity for neuronal physiology. In the section on intercellular communication, the chapter on neurotransmitter secretion has also been rewritten to reflect the new level of understanding of secretion that has resulted from the identification of many of the molecular players in vesicle fusion and exocytosis. The other chapters in this section have also been fully revised to incorporate new information resulting from the cloning and characterization of the multitude of glutamate receptors as well as to describe novel elements of intracellular signaling pathways in neurons and other cells. Finally, the last section has been substantially updated to reflect the recent successes of molecular studies of development and plasticity. As more and more of the molecular entities that are essential for neuronal development and adult plasticity are identified and characterized, phenomena that previously could be studied only at the descriptive level can now be explained in greater depth.

Neuronal Cell Death

Neuronal Cell Death
Author :
Publisher : Humana
Total Pages : 0
Release :
ISBN-10 : 1493944649
ISBN-13 : 9781493944644
Rating : 4/5 (49 Downloads)

Synopsis Neuronal Cell Death by : Laura Lossi

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.

Biochemistry and Cell Culture

Biochemistry and Cell Culture
Author :
Publisher :
Total Pages : 505
Release :
ISBN-10 : 1283836963
ISBN-13 : 9781283836968
Rating : 4/5 (63 Downloads)

Synopsis Biochemistry and Cell Culture by : P. Michael Conn

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields.