Molecular Cytology, Volume 2: Cell Interactions deals with the morphology and biochemistry of the cell, with emphasis on the more dynamic aspects of cytology. It looks at gene transfer in somatic cells, nucleocytoplasmic interactions in oocytes and eggs, and cell differentiation, transformation, malignancy, aging, and death. Organized into four chapters, this volume begins with a discussion of nucleocytoplasmic interactions in somatic cells and unicellular organisms. The next chapter examines the experimental interventions at early stages in the egg cytoplasm with reference to Xenopus oocytes, as well as oogenesis, the structure and composition of the cytoplasm and the nucleus, fertilization of sea urchin eggs, and the nuclear determinants of early embryogenic development. Additionally, a chapter explains the mechanisms underlying cell senescence, arrest of cell growth, and cell death; the mechanisms of cell differentiation as the normal outcome of embryonic development; the morphological and biochemical changes that occur in cells when they become senescent; and the metastasis of cancer cells. The book concludes with a chapter that presents a few general ideas about biochemical cytology. This book is a valuable reference for cell biologists, biochemists, cytologists, advanced students, research workers, and laypersons interested in learning the fundamentals of descriptive cytology, biochemistry, embryology, genetics, and molecular biology.

Molecular Cytology presents an integrated version about the morphology and biochemistry of the cell. This two-volume book focuses on the dynamic aspects of cytology and on the nucleocytoplasmic interactions in unicellular organisms and eggs. The first chapter covers the history of cell, cytology, and nucleic acids, as well as the uniformity and diversity in cell. The book then discusses various methods used in cell biology, including optical, cytochemical, biological, biochemical, and biophysical techniques. It also examines the activities of cytoplasm and nucleus during interphase. The final chapter describes various phases of the cell cycle, the structure of metaphase chromosomes, the molecular organization of the mitotic apparatus, and the cytokinesis, with emphasis on the main mitotic abnormalities. With the aim of linking the morphology and biochemistry of the cell, this book is intended for advanced students, research workers, biochemists, and cytologists who wish to broaden their knowledge in cell.

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

International Review of Cytology

Plants interact with a large number of microoganisms which have a major impact on their growth either by establishing mutually beneficial symbiotic relationships or by developing as pathogens at the expense of the plant with deleterious effects. These microorganisms differ greatly not only in their nature (viruses, phytoplasmas, bacteria, fungi, nematodes, ... ) but also in the way they contact, penetrate and invade their host. Histology and cytology have brought an essential contribution to our knowledge of these phenomena. They have told us for instance, how specialized structures of the pathogen are often involved in the adhesion and penetration into the plant, how the interface between both organisms is finely arranged at the cellular level, or what structural alterations affect the infected tissues. They have thus set the stage for the investigations of the underlying molecular mechanisms could be undertaken. Such investigations have been remarkably successful in the recent years, expanding considerably our understanding of plant-microorganism interactions in terms of biochemical changes, rapid modifications of enzymatic activities, coordinated gene activation, signal reception and transduction. Biochemistry, molecular biology and cellular physiology have taken precedence in the phytopathologist's set of methods.

International Review of Cytology presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Key Features* Each volume provides up-to-date information and directions for future research* Authored by some of the foremost international scientists in the field * Fully illustrated in color and black and white