Microdroplet technology has recently emerged to provide new and diverse applications via microfluidic functionality, especially in various areas of biology and chemistry. This book, then, gives an overview of the principle components and wide-ranging applications for state-of-the-art of droplet-based microfluidics. Chapter authors are internationally-leading researchers from chemistry, biology, physics and engineering that present various key aspects of micrdroplet technology -- fundamental flow physics, methodology and components for flow control, applications in biology and chemistry, and a discussion of future perspectives. This book acts as a reference for academics, post-graduate students, and researcher wishing to deepen their understand of microfluidics and introduce optimal design and operation of new droplet-based microfluidic devices for more comprehensive analyte assessments.

This book introduces a unique 3D printing method that prints metal parts by ejecting metal micro-droplets: a low-cost, contactless, and environmentally friendly 3D printing technology. This book follows a deductive approach to describe the fundamentals of metal droplet-based 3D printing and reveal the relationships and unique features among multiple specific techniques used in droplet-based 3D printing. It starts with a general description of the principles and techniques involved in this technology and then focuses on the details of several specific metal droplet-ejection methods. Next, it puts forward various specific 3D printing techniques and illustrates their applications. This book is a valuable reference for scholars and researchers who work on metal 3D printing and other related areas. It is also used as a textbook for college graduate courses in mechanical manufacturing and material processing.

This authoritative textbook offers in-depth coverage of all aspects of molecular pathology practice and embodies the current standard in molecular testing. Since the successful first edition, new sections have been added on pharmacogenetics and genomics, while other sections have been revised and updated to reflect the rapid advances in the field. The result is a superb reference that encompasses molecular biology basics, genetics, inherited cancers, solid tumors, neoplastic hematopathology, infectious diseases, identity testing, HLA typing, laboratory management, genomics and proteomics. Throughout the text, emphasis is placed on the molecular variations being detected, the clinical usefulness of the tests and important clinical and laboratory issues. The second edition of Molecular Pathology in Clinical Practice will be an invaluable source of information for all practicing molecular pathologists and will also be of utility for other pathologists, clinical colleagues and trainees.

Clinical Genomics provides an overview of the various next-generation sequencing (NGS) technologies that are currently used in clinical diagnostic laboratories. It presents key bioinformatic challenges and the solutions that must be addressed by clinical genomicists and genomic pathologists, such as specific pipelines for identification of the full range of variants that are clinically important. This book is also focused on the challenges of diagnostic interpretation of NGS results in a clinical setting. Its final sections are devoted to the emerging regulatory issues that will govern clinical use of NGS, and reimbursement paradigms that will affect the way in which laboratory professionals get paid for the testing. - Simplifies complexities of NGS technologies for rapid education of clinical genomicists and genomic pathologists towards genomic medicine paradigm - Tried and tested practice-based analysis for precision diagnosis and treatment plans - Specific pipelines and meta-analysis for full range of clinically important variants

This book provides readers with new paradigms on the mutation discovery in the post-genome era. The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated the progress in positional cloning of genes from mutated models. In 2002, the Mouse Genome Sequencing Consortium stated that “The availability of an annotated mouse genome sequence now provides the most efficient tool yet in the gene hunter's toolkit. One can move directly from genetic mapping to identification of candidate genes, and the experimental process is reduced to PCR amplification and sequencing of exons and other conserved elements in the candidate interval. With this streamlined protocol, it is anticipated that many decades-old mouse mutants will be understood precisely at the DNA level in the near future.” The implication of such a statement should be similar to the identification of mutated genes from human diseases and animal models, when genome sequencing is completed for them. More than five years have passed, but genes in many human diseases and animal models have not yet been identified. In some cases, the identification of the mutated genes has been a bottleneck, because the genetic mechanism holds the key to understand the basis of the diseases. However, an integrative strategy, which is a combination of genetic mapping, genome resources, bioinformatics tools, and high throughput technologies, has been developed and tested. The classic paradigm of positional cloning has evolved with completely new concepts of genomic cloning and protocols. This book describes new concepts of gene discovery in the post-genome era and the use of streamlined protocols to identify genes of interest. This book helps identify not only large insertions/deletions but also single nucleotide mutations or polymorphisms that regulate quantitative trait loci (QTL).

This book describes the rational design, development and application of nucleic acid amplification strategies for biosensing, bioimaging and biomedicine. It consists of fifteen chapters demonstrating the use of these strategies in various areas, including fluorescence techniques, Chemiluminescence biosensors, electrochemiluminescence biosensors, colorimetric assays, surface plasmon resonance technologies, electrochemical DNA sensors, photoelectrochemical biosensor, nanopore sensors, quartz crystal microbalance, fluorescence imaging, surface-enhanced Raman spectroscopy, in vitro and in vivo metal ions detection, theranostics and microdroplet chips. Offering a collection of reviews illustrating the latest advances in biochemical analysis and therapeutics, the book shares valuable insights into current challenges and future prospects, making it a valuable resource for a wide readership in the various fields of biosensing, bioimaging and biomedicine.

Molecular Analysis and Genome Discovery, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications are provided alongside practical information on current techniques currently being researched and developed. Each chapter offers an up-to-date analysis of the subject encompassing the very latest technology platforms and is an essential reference for researchers in the field looking for an up-to-date overview of the subject. The book will also be an indispensable resource for those working in the biotechnology and pharmaceutical industries. New for this edition: chapters on Genotyping through Mutation Detection; Differential Gene Expression; Haplotyping and Molecular Profiling.

This book delves into the biotechnological applications of Quorum sensing (QS)- a peculiar gene-regulatory process of some microorganisms. Quorum Sensing allows a large bacterial population to work together in a coordinated manner to carry out metabolic activities, which individual bacterium cannot. The different chapters describe how, associating bioremediation process with energy generation is an economical proposal, for reducing pollution and managing biowastes. The book discusses how QS can be exploited for biotechnological applications in generating bioproducts, bioenergy, bioremediation, biosensors, health and agricultural activities. It further highlights how QS is becoming an integral part of synthetic biology for genetic circuits for producing: (i) novel products, (ii) biosensors, (iii) bioactive molecules, etc. The book is divided into different sections for a clear understanding of the applicability of QS in, the Environment, Energy, Agriculture and Health sectors.

Intelligent transport systems are on the increase. They employ a variety of technologies, from basic management systems to more advanced application systems, with information technology – including wireless communication, computational technologies, floating car data/cellular data such as sensing technologies and video vehicle detection – playing a major role. This book presents the proceedings of the 2nd International Conference on Information Technology and Intelligent Transportation Systems (ITITS 2017), held in Xi’an, People's Republic of China, in June 2017. The conference provides a platform for professionals and researchers from industry and academia to present and discuss recent advances in the field of information technology and intelligent transportation systems; organizations and researchers involved in these fields, including distinguished academics from around the world, explore theoretical and applied topics such as emergency vehicle notification systems, automatic road enforcement, collision avoidance systems and cooperative systems. ITITS 2017 received more than 200 papers from 4 countries, and the 65 accepted papers appear in this book, which will be of interest to all those involved with the development of intelligent transport systems.

Get the expert guidance you need to offer your patients the best possible outcomes with Hematology: Basic Principles and Practice, 7th Edition. This thoroughly up-to-date text contains both unparalleled scientific content and must-know clinical guidance, so you can enhance your problem-solving skills and make optimal use of the newest diagnostic techniques and therapeutic options in this fast-changing field. Delivers state-of-the-art information and guidance from editors and global contributors who are at the forefront of their respective subspecialty areas Features sweeping content updates throughout, including basic science research which serves as a foundation for modern hematology, recent advances in stem cell transplantation, clinical advances in the treatment of each of the hematologic malignancies, immune checkpoint inhibitors, molecular diagnostics, transfusion medicine, and much more Includes several new chapters including Epigenetics and Epigenomics, Stem Cell Model of Hematologic Diseases, Multiple Myeloma, IND Enabling Processes for Cell-Based Therapies, and Immune Checkpoint Blockade in Hematologic Malignancies New Virtual Microscope with the ability to zoom in on high-quality digital hematopathology slides and frequent content updates accessible anywhere, any time on your favorite digital device Expert ConsultTM eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, Q&As, and references from the book on a variety of devices Delivers state-of-the-art information and guidance from editors and global contributors who are at the forefront of their respective subspecialty areas. Features sweeping content updates throughout, including basic science research which serves as a foundation for modern hematology, recent advances in stem cell transplantation, clinical advances in the treatment of each of the hematologic malignancies, immune checkpoint inhibitors, molecular diagnostics, transfusion medicine, and much more. Includes several new chapters including Epigenetics and Epigenomics, Stem Cell Model of Hematologic Diseases, Multiple Myeloma, IND Enabling Processes for Cell-Based Therapies, and Immune Checkpoint Blockade in Hematologic Malignancies. New Virtual Microscope with the ability to zoom in on high-quality digital hematopathology slides and frequent content updates accessible anywhere, any time on your favorite digital device. Expert ConsultTM eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, Q&As, and references from the book on a variety of devices.