This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1852 edition. Excerpt: ... CHAPTER XII. How rarely is it--more rarely than when, in the midst of the broad Atlantic, vessel meets vessel--that man can ask his heart, "What cheer V and receive for answer, "Alls well." When Julian Ludlow retired to his chamber that night--when the door was closed, and, by the kindly but officious care of Mr. Westwoods valet, he was hurried to bed more rapidly than he desired--he lay awake for upwards of an hour, notwithstanding the exhaustion of illness, and the unusual fatigue. The cry rose up within him--" What cheer?" But the answer never was "Alls well." At one time, it was "Breakers a-head " and at others, the same voice spoke of dangers all around. Every one has felt how different is the aspect of the same facts and circumstances at each varying hour of the day; how differently we look upon our fate and future in the merry morning, in the sunny noon, and in the dull, silent night; and there left alone, with the dim watch-lamp winking in the chimney, almost all that was bright and cheering in the events of that day passed away from Julians eyes like unreal pageants; and nothing but the dark, and the coarse, and the heavy remained. Sometimes he could hardly believe it real; that he had spoken as he had; that Mary had so answered him; that their almost insane love had been breathed to each other; that their hearts had found voice in sounds beyond recal. It seemed a dream--a vision, which could have no truth in it; and yet it came back and back upon him with an awful sense of all its consequences. Was not the love itself rash, mad, hopeless % Had not the avowal of it been wrong, base, ungrateful? "What would Mr. Westwood think--he who had loaded him with kindness--he who had fostered, with such tenderness, his youth, promoted...

Modern Methods in Carbohydrate Synthesis presents in one volume a sequence of chapters leading from classical methods through to today's newest state-of -the-art technology for oligosaccharide synthesis. It places particular emphasis on the most recent breakthroughs in the field, including emerging technologies for both oligosaccharide and glycoconjugate synthesis. Chapters describing the synthesis of increasingly important glycosidic linkage analogs, as well as the oligosaccharides containing derivatives and analogs of natural sugars are included. While chemical-synthetic methods constitute the major part of the book, completing the volume is a section on the rapidly expanding and important field of enzymatic synthesis, also covering combined chemical and enzymatic synthesis. Chapters are written by leading experts in the field. Wherever possible, methods of synthesis are provided in sufficient detail to allow the reader to implement the techniques described. More than 1700 references are provided in the 21 chapters comprising the book. This volume should provide a wealth of information to a large number of synthetic organic chemists, medicinal chemists, protein chemists, biochemists, glycobiologists and cell biologists, including students in these fields.

Methods in Carbohydrate Chemistry, Volume VIII: General Methods describes the principles and mode of operation of general methods for the analysis and structural characterization of carbohydrates. This book is organized into two sections encompassing 50 chapters. The first section highlights the methods on automated chromatographic techniques; enzymic and other methods for structural analysis of polysaccharides. This section also explores the application of 13C NMR spectroscopy to carbohydrate chemistry. The second section describes the synthesis of deoxy and branched-chain sugars, 1,2-transglycosides, de-N- and de-O-sulfation, and de-N-acetylation of polysaccharides. This book is an invaluable source for organic and analytical chemists, as well as for carbohydrate scientists and researchers.

Detailing commonly used methods and procedures, this reference discusses the reactions and derivative forms of carbohydrates. Preparative Carbohydrate Chemistry covers the formation, cleavage, and reactions of derivatives and illustrates bond-forming reactions of SN2 types, free radicals, chain extensions, and branching. The contents include: sugar derivatives; selected reactions in carbohydrate chemistry; chemical synthesis of oligosaccharides and O-and N -glycosyl compounds; enzymatic synthesis of sialic acid, KDO, and related deoxyulosonic acids, and of oligosaccharides; synthesis of -glycosyl compounds; carbocycles from carbohydrates; and total synthesis of sugars from non-sugars. This authoritative reference offers relevant chapters on reactions and derivative forms of carbohydrates, including commonly used methods as well as new experimental procedures. It also contains insightful chapter commentaries and succinct topic histories.

A practical bench-side reference for carbohydrate chemistry Methods in Carbohydrate Chemistry: Lipopolysaccharides, Separation and Analysis, Glycosylated Polymers (Volume 9) presents proven techniques for working with carbohydrates in the lab. Topic experts contribute insights and protocols for membrane isolation and purification, glycoprotein synthesis, and carbohydrate immobilization, with detailed guidance on chromatographic, chemical, enzymatic, and physical methods of separation and analysis. Helpful flow charts provide easy bench-side reference, while proven methods allow for predictable, repeatable results. Anyone who encounters carbohydrates in the lab will find value in this clear, practical reference.

A growing awareness of the relationship between diet and health has led to an increasing demand for food products that support health beyond simply providing basic nutrition. Digestive health is the largest segment of the burgeoning functional food market worldwide. Incorporation of bioactive oligosaccharides into foods can yield health benefits in the gastrointestinal tract and other parts of the body that are linked via the immune system. Because oligosaccharides can be added to a wide variety of foodstuffs, there is much interest within the food industry in incorporating these functional ingredients into healthy food products. Moreover, other areas such as pharmaceuticals, bioenergy and environmental science can exploit the physicochemical and physiological properties of bioactive oligosaccharides too. There is therefore a considerable demand for a concentrated source of information on the development and characterization of new oligosaccharides with novel and/or improved bioactivities. Food Oligosaccharides: Production, Analysis and Bioactivityis a comprehensive reference on the naturally occurring and synthesised oligosaccharides, which will enable food professionals to select and use these components in their products. It is divided into three sections: (i) Production and bioactivity of oligosaccharides, (ii) Analysis and (iii) Prebiotics in Food Formulation. The book addresses classical and advanced techniques to structurally characterize and quantitatively analyse food bioactive oligosaccharides. It also looks at practical issues faced by food industry professionals seeking to incorporate prebiotic oligosaccharides into food products, including the effects of processing on prebiotic bioavailability. This book is essential reading for food researchers and professionals, nutritionists and product developers working in the food industry, and students of Food Science with an interest in functional foods.

Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms. The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available. - Includes a variety of analytical methods and how to apply the methods using examples from specific case studies - Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides - Contains easy-to-follow protocols with detailed explanations for self-guidance - Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment

The fields of glycochemistry and glycoscience are rich and varied and where much can be learned from Nature. As Nature is not always able to produce carbohydrates in quantities useful for not only in research but also as therapeutic agents, new ways need to be found to optimize the yield. This book presents an overview of the latest developments in the field of carbohydrates, ranging from de-novo approaches via cyclodextrin chemistry to the synthesis of such highly complex glycoconjugates as glycosphingolipids and GPI anchors. The main emphasis remains on the synthetic aspects making the book an excellent source of information for those already involved in carbohydrate chemistry, as well as for those organic chemists who are beginners in this field. Equally of interest to synthetic chemists, as well as medicinal chemists and biochemists.

Methods of Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Readers will especially benefit from the step-by-step explanation of the lab procedures, as well as background information for using these methods effectively and analyzing data. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background informaton, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. Written for the researcher, but welcoming to those new to soil enzymology, the introduction includes conceptual information to orient those who are not familiar with these methods but want to use them. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.