Manual of Assisted Reproductive Technologies and Clinical Embryology aims to discuss the relevance of science of reproductive biology in modern-day Assisted Reproductive Technologies and their practical applications. The readers can learn and master the large number of sophisticated techniques which form the backbone of the fascinating and growing field of human assisted reproduction. The subject is vast and has been covered over 83 chapters. All the chapters are dealt by the experts of concerned fields. Principles and protocols pertaining to laboratory maintenance, culture media, cryofreezing of gametes, embryos, and genital tissues have been dealt with at length. This book is an invaluable reference book for the clinicians, reproductive biologists and embryologists.

The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.

Personnel working in assisted reproductive technology often lack the opportunities for dedicated training in the specialized techniques and technologies required for the procedures. As such, success in the form of live birth rates can range from over 50% to less than 10% per treatment cycle. This comprehensive introductory textbook is an essential resource for trainee embryologists, medical students and nurses. The recent revolutions in biotechnology and molecular biology involved in delivering assisted reproductive services are thoroughly discussed. Basic knowledge such as the development and physiology of both male and female reproductive systems is covered, with practical aspects of IVF including gamete and embryo manipulation, cryopreservation and genetic testing explained in detail. A full description of the optimal structure and management of the IVF laboratory is given, helping ensure procedures are safe and effective. Extensive and highly detailed colour illustrations bring the content to life and aids readers in their understanding.

Whilst assisted reproduction techniques (ART) have become increasingly successful and largely standardized, there is still only a partial understanding of what constitutes a 'true' embryo environment. Replicating the varying physiological conditions of the in-vivo environment that the embryo travels through in the in-vitro culture is still a major challenge in ART. This practical volume details how to organize and operate an IVF laboratory in order to mimic these conditions for successful embryo culture. Environments and equipment that are essential for running safe and efficient facilities such as maintaining good air quality and hygiene protocols, and utilizing an effective layout are covered in detail. Other chapters discuss the different consumables needed, optimal handling techniques and parameter monitoring systems, as well as recent advances in the area including artificial intelligence and automation. This is an indispensable guide to understanding the background science of culturing embryos, crucial to successful outcomes in ART.

Textbook of Assisted Reproductive Technologies has become a classic comprehensive reference for the whole team at the IVF clinic. The fourth edition comes more conveniently as a set of two separate volumes, one for laboratory aspects and the other for clinical applications. The text has been extensively revised, with the addition of several important new contributions on clinical applications, including new chapters on lifestyle factors, tailored ovarian stimulation, frozen-thawed embryo transfer, viral disease, and religious perspectives. As before, methods, protocols, and techniques of choice are presented by eminent international experts. Also available - Textbook of Assisted Reproductive Technologies, Volume One - Laboratory Perspectives Textbook of Assisted Reproductive Technologies, Two Volume Set

VOLUME 1: INFERTILITY SECTION 1: ANATOMY AND PHYSIOLOGY 1. Anatomy of the Reproductive System 2. Regulation and Physiology of Menstrual Cycle 3. Oogenesis and Folliculogenesis 4. Spermatogenesis 5. Fertilization and Embryogenesis 6. Implantation 7. Embryo Endometrial Crosstalk and Endometrial Receptivity SECTION 2: REPRODUCTIVE ENDOCRINOLOGY 8. Synthesis and Metabolism of Steroid Hormones 9. Puberty and Aberrations 10. Amenorrhea 11. Endocrine Disorders Affecting Reproduction 12. Hirsutism 13. Luteal Phase Defect 14. Anovulation 15. Declining Fertility SECTION 3: COMBINED TOPICS 16. Evaluation of Infertility 17. Immunology and Infertility 18. Cytogenetics and Subfertility 19. Obesity and Infertility 20. Unexplained Infertility 21. Fertility Preservation 22. Counseling in Infertility 23. Assisted Reproductive Technology in Patients with Chronic Medical Disorders SECTION 4: MALE INFERTILITY 24. Etiopathogenesis of Male Infertility 25. Clinical and Endocrinological Evaluation of Infertile Male 26. Sexual Dysfunction in Male Infertility 27. Ultrasound in Male Infertility 28. Medical Management of Male Infertility 29. Azoospermia: Evaluation and Management 30. Varicocele and Infertility 31. Spinal Cord Injuries and Male Infertility 32. Algorithms for Genetic Evaluation of Infertile Males SECTION 5: FEMALE FACTOR INFERTILITY 33. Uterine Factors in Infertility 34. Tubal Factors in Infertility 35. Infections and Infertility 36. Tuberculosis and Infertility 37. Sonoendocrinology and Cycle Monitoring Assisted Reproduction Technology 38. Transvaginal Ultrasound and Doppler in Infertility 39. Polycystic Ovary Syndrome 40. Assessment of Ovarian Reserve 41. Endometriosis 42. Endoscopy in Infertility 43. Reconstructive Surgeries Enhancing Fertility SECTION 6: INTRAUTERINE INSEMINATION 44. Intrauterine Insemination 45. Optimizing Success in Intrauterine Insemination SECTION 7: OVARIAN STIMULATION 46. Drugs for Ovarian Stimulation 47. Ovulation Induction and Ovarian Stimulation Protocols 48. Role of Adjuvants in Ovarian Stimulation 49. Gonadotropinreleasing Hormone Analogs 50. Monitoring of Ovarian Stimulation 51. Ovulation Trigger 52. Individualized Controlled Ovarian Stimulation 53. In Vitro Fertilization Lite 54. Role of Luteinizing Hormone in Ovarian Stimulation 55. Anesthesia in Assisted Reproductive Techniques 56. Oocyte Retrieval. 57. Embryo Transfer 58. Troubleshooting in Assisted Reproductive Technology 59. Luteal Phase Support SECTION 8: DILEMMA IN ART 60. Poor Responder 61. Recurrent Implantation Failure 62. Empty Follicle Syndrome 63. Role of Aneuploidy Screening in Preimplantation Embryos 64. Preimplantation Genetic Testing of Embryos 65. Epigenetics and Assisted Reproductive Technology SECTION 9: COMPLICATIONS IN ART 66. Ovarian Hyperstimulation Syndrome 67. Ectopic Pregnancy 68. Multipleorder Births SECTION 10: THIRD PARTY REPRODUCTION 69. Oocyte and Sperm Donation 70. Surrogacy in Assisted Reproductive Technology 71. Assisted Reproductive Technology Guidelines 72. Adoption 73. LGBTQ and Fertility 74. Transgender Population and Fertility SECTION 11: OUTCOME FOLLOWING ASSISTED REPRODUCTIVE TECHNIQUE 75. Maternal and Fetal Outcomes Following Assisted Reproductive Technique 76. Early Pregnancy Scan 77. Recurrent Pregnancy Loss: From Diagnostic Dilemmas to Clinical Decisions SECTION 12: RECENT ADVANCES 78. Bioengineered Human Endometrium In Vitro. 79. Recent Trends in A...

Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.

The new edition of this infertility manual has been fully revised to provide clinicians with the latest advances in the diagnosis and management of infertility. Divided into seven sections, the book provides step by step guidance on each stage of the process, from initial examination and identifying the causes of infertility in both females and males, to ovarian stimulation and assisted reproduction techniques. The final section is dedicated to laboratory management covering topics such as follicular fluid screening and oocyte assessment, culture systems, and cryopreservation. The fourth edition includes new chapters on molecular mechanisms such as endometrial receptivity, and implantation; and current trends such as the embryoscope and assisted hatching. The comprehensive text is further enhanced by case studies, clinical photographs, diagrams, flowcharts and tables. Key points Fully revised, new edition providing latest advances in diagnosis and management of infertility Fourth edition features new chapters on molecular mechanisms and current trends Highly illustrated with clinical images, flowcharts and tables Previous edition (9788184486179) published in 2009

20 interactive DVDs featuring over 130 videos providing a comprehensive overview of Assisted Reproductive Technologies (ART). Accompanying book covers In Vitro Fertilisation (IVF).

An ultrasound imaging guide for use in assisted reproduction, heavily illustrated, with practical tips for obtaining high-quality images.