Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series

Structure and Intrinsic Disorder in Enzymology offers a direct, yet comprehensive presentation of the fundamental concepts, characteristics and functions of intrinsically disordered enzymes, along with valuable notes and technical insights powering new research in this emerging field. Here, more than twenty international experts examine protein flexibility and cryo-enzymology, hierarchies of intrinsic disorder, methods for measurement of disorder in proteins, bioinformatics tools for predictions of structure, disorder and function, protein promiscuity, protein moonlighting, globular enzymes, intrinsic disorder and allosteric regulation, protein crowding, intrinsic disorder in post-translational, and much more. Chapters also review methods for study, as well as evolving technology to support new research across academic, industrial and pharmaceutical labs. - Unifies the roles of intrinsic disorder and structure in the functioning of enzymes and proteins - Examines a range of enzyme and protein characteristics, their relationship to intrinsic disorder, and methods for study - Features chapter contributions from international leaders in the field

History of surface phenomena offers critical and detailed examination and assessment of modern theories, focusing on statistical mechanics and application of results in mean-field approximation to model systems. 1989 edition.

Droplets of Life: Membrane-Less Organelles, Biomolecular Condensates, and Biological Liquid–Liquid Phase Separation provides foundational information on the biophysics, biogenesis, structure, functions, and roles of membrane-less organelles. The study of liquid–liquid phase separation has attracted a lot of attention from disciplines such as cell biology, biophysics, biochemistry, and others trying to understand how, why, and what roles these condensates play in homeostasis and disease states in living organisms. This book's editor recruited a group of international experts to provide a current and authoritative overview of all aspects associated with this exciting area. Sections introduce membrane-less organelles (MLOs) and biomolecular condensates; MLOs in different sizes, shapes, and composition; and the formation of MLOs due to phase separation and how it can tune reactions, organize the intracellular environment, and provide a role in cellular fitness. . - Presents the first book to establish the foundations of this exciting research area - Combines biophysics, structural and cell biology, and biochemistry perspectives into a single volume - Edited and authored by world-leading scientists - Covers basic physical and biological principles and health and disease implications

This book surveys the models for the origin of life and presents a new model starting with shaped droplets and ending with life as polygonal Archaea; it collects the most published micrographs of Archaea (discovered only in 1977), which support this conclusion, and thus provides the first visual survey of Archaea. Origin of Life via Archaea’s purpose is to add a new hypothesis on what are called “shaped droplets”, as the starting point, for flat, polygonal Archaea, supporting the Vesicles First hypothesis. The book contains over 6000 distinct references and micrographs of 440 extant species of Archaea, 41% of which exhibit polygonal phenotypes. It surveys the intellectual battleground of the many ideas of the origin of life on earth, chemical equilibrium, autocatalysis, and biotic polymers. This book contains 17 chapters, some coauthored, on a wide range of topics on the origin of life, including Archaea’s origin, patterns, and species. It shows how various aspects of the origin of life may have occurred at chemical equilibrium, not requiring an energy source, contrary to the general assumption. For the reader’s value, its compendium of Archaea micrographs might also serve many other interesting questions about Archaea. One chapter presents a theory for the shape of flat, polygonal Archaea in terms of the energetics at the surface, edges and corners of the S-layer. Another shows how membrane peptides may have originated. The book also includes a large table of most extant Archaea, that is searchable in the electronic version. It ends with a chapter on problems needing further research. Audience This book will be used by astrobiologists, origin of life biologists, physicists of small systems, geologists, biochemists, theoretical and vesicle chemists.

A clear and intuitive introduction to the physics of charged macromolecules, from fundamentals to the latest research developments.

Covering the elementary aspects of the physics of phases transitions and the renormalization group, this popular book is widely used both for core graduate statistical mechanics courses as well as for more specialized courses. Emphasizing understanding and clarity rather than technical manipulation, these lectures de-mystify the subject and show precisely "how things work." Goldenfeld keeps in mind a reader who wants to understand why things are done, what the results are, and what in principle can go wrong. The book reaches both experimentalists and theorists, students and even active researchers, and assumes only a prior knowledge of statistical mechanics at the introductory graduate level.Advanced, never-before-printed topics on the applications of renormalization group far from equilibrium and to partial differential equations add to the uniqueness of this book.

Scattering Methods in Structural Biology, Part B, Volume 676 in the Methods in Enzymology serial, highlights advances in the field, presenting chapters on Quality controls, Refining biomolecular structures and ensembles by SAXS-driven molecular dynamics simulations, Data analysis and modelling of small-angle scattering data with contrast variation, Observing protein degradation in solution by the PAN-20S proteasome complex: state-of-the-art and future perspectives of TR-SANS as a complementary tool to NMR, crystallography and Cryo-EM, Extracting structural insights from chemically-specific soft X-ray scattering, Reconstruction of 3D density of biological macromolecules from solution scattering, ATSAS- present state and new developments in computational methods, and much more. Additional chapters cover Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles (FoXSDock and MultiFoXS), Validation of macromolecular flexibility in solution by SAXS, Combining NMR, SAXS and SANS to characterize the structure and dynamics of protein complexes, Application of Molecular Simulation Methods to Analyze SAS Data, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology serial - Updated release includes the latest information on Small Angle Scattering Methods for Structural Interpretation