The most mature, widespread sorting technology, fluorescence-activated cell sorting (FACS), offers high throughput and sorts predicated on a wide range of phenotypes that can be conveyed through average cellular fluorescence or light scattering levels. However, FACS cannot detect a wide range of phenotypes easily observable through microscopy, such as details of cell morphology, sub-cellular localization, and temporal fluorescence expression with single-cell resolution. Presently, there is no widespread, user-friendly technique to sort cells following microscopy. Commercial solutions offering this capability are prohibitively expensive to individual labs, and are most likely to be found only in well-resourced core facilities. This thesis presents the design, implementation, and testing of three new technologies for simple, inexpensive, viable, image-predicated cell sorting that can be disseminated to individual labs. For anchorage-dependent cells, we have implemented two technologies. The first is a method termed polymerization-activated cell sorting, or PACS, that permits the selective hydrogel-mediated photo-encapsulation of undesired cells in a culture dish and the retrieval of desired cells using enzyme-mediated release. The second is a method termed radical-activated cell sorting, or RACS, that permits the light-mediated killing of undesired cells in a culture dish through photo patterning of radical toxicity. For non-anchorage-dependent cells, we have developed optofluidic cell sorting, or optoflucs. In this approach, cells are injected into a microfluidic device where they are allowed to settle into an array of cell-sized microwells. A laser is then used to optically levitate desired cells out of the array and into a flow stream for collection. This thesis demonstrates> 100-fold enrichment of target cell populations and up to 89% output purity following a single round of image-predicated, viable cell sorting. In addition, this thesis demonstrates examples of sorts predicated on fluorescence localization, a phenotype that cannot be sorted using FACS. These results, coupled with the minimal expense and simplicity of these technologies make these techniques appealing for widespread adoption by individual biology labs.

The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Artificial Intelligence (AI) in Healthcare is more than a comprehensive introduction to artificial intelligence as a tool in the generation and analysis of healthcare data. The book is split into two sections where the first section describes the current healthcare challenges and the rise of AI in this arena. The ten following chapters are written by specialists in each area, covering the whole healthcare ecosystem. First, the AI applications in drug design and drug development are presented followed by its applications in the field of cancer diagnostics, treatment and medical imaging. Subsequently, the application of AI in medical devices and surgery are covered as well as remote patient monitoring. Finally, the book dives into the topics of security, privacy, information sharing, health insurances and legal aspects of AI in healthcare. - Highlights different data techniques in healthcare data analysis, including machine learning and data mining - Illustrates different applications and challenges across the design, implementation and management of intelligent systems and healthcare data networks - Includes applications and case studies across all areas of AI in healthcare data

This book examines issues and implications of digital and social media marketing for emerging markets. These markets necessitate substantial adaptations of developed theories and approaches employed in the Western world. The book investigates problems specific to emerging markets, while identifying new theoretical constructs and practical applications of digital marketing. It addresses topics such as electronic word of mouth (eWOM), demographic differences in digital marketing, mobile marketing, search engine advertising, among others. A radical increase in both temporal and geographical reach is empowering consumers to exert influence on brands, products, and services. Information and Communication Technologies (ICTs) and digital media are having a significant impact on the way people communicate and fulfil their socio-economic, emotional and material needs. These technologies are also being harnessed by businesses for various purposes including distribution and selling of goods, retailing of consumer services, customer relationship management, and influencing consumer behaviour by employing digital marketing practices. This book considers this, as it examines the practice and research related to digital and social media marketing.

The Bulletin of the Atomic Scientists is the premier public resource on scientific and technological developments that impact global security. Founded by Manhattan Project Scientists, the Bulletin's iconic "Doomsday Clock" stimulates solutions for a safer world.

"The ongoing COVID-19 pandemic marks the most significant, singular global disruption since World War II, with health, economic, political, and security implications that will ripple for years to come." -Global Trends 2040 (2021) Global Trends 2040-A More Contested World (2021), released by the US National Intelligence Council, is the latest report in its series of reports starting in 1997 about megatrends and the world's future. This report, strongly influenced by the COVID-19 pandemic, paints a bleak picture of the future and describes a contested, fragmented and turbulent world. It specifically discusses the four main trends that will shape tomorrow's world: - Demographics-by 2040, 1.4 billion people will be added mostly in Africa and South Asia. - Economics-increased government debt and concentrated economic power will escalate problems for the poor and middleclass. - Climate-a hotter world will increase water, food, and health insecurity. - Technology-the emergence of new technologies could both solve and cause problems for human life. Students of trends, policymakers, entrepreneurs, academics, journalists and anyone eager for a glimpse into the next decades, will find this report, with colored graphs, essential reading.

The classic work on the evaluation of city form. What does the city's form actually mean to the people who live there? What can the city planner do to make the city's image more vivid and memorable to the city dweller? To answer these questions, Mr. Lynch, supported by studies of Los Angeles, Boston, and Jersey City, formulates a new criterion—imageability—and shows its potential value as a guide for the building and rebuilding of cities. The wide scope of this study leads to an original and vital method for the evaluation of city form. The architect, the planner, and certainly the city dweller will all want to read this book.

Sensors for Health Monitoring discusses the characteristics of U-Healthcare systems in different domains, providing a foundation for working professionals and undergraduate and postgraduate students. The book provides information and advice on how to choose the best sensors for a U-Healthcare system, advises and guides readers on how to overcome challenges relating to data acquisition and signal processing, and presents comprehensive coverage of up-to-date requirements in hardware, communication and calculation for next-generation uHealth systems. It then compares new technological and technical trends and discusses how they address expected u-Health requirements. In addition, detailed information on system operations is presented and challenges in ubiquitous computing are highlighted. The book not only helps beginners with a holistic approach toward understanding u-Health systems, but also presents researchers with the technological trends and design challenges they may face when designing such systems. - Presents an outstanding update on the use of U-Health data analysis and management tools in different applications, highlighting sensor systems - Highlights Internet of Things enabled U-Healthcare - Covers different data transmission techniques, applications and challenges with extensive case studies for U-Healthcare systems

Technologies collectively called omics enable simultaneous measurement of an enormous number of biomolecules; for example, genomics investigates thousands of DNA sequences, and proteomics examines large numbers of proteins. Scientists are using these technologies to develop innovative tests to detect disease and to predict a patient's likelihood of responding to specific drugs. Following a recent case involving premature use of omics-based tests in cancer clinical trials at Duke University, the NCI requested that the IOM establish a committee to recommend ways to strengthen omics-based test development and evaluation. This report identifies best practices to enhance development, evaluation, and translation of omics-based tests while simultaneously reinforcing steps to ensure that these tests are appropriately assessed for scientific validity before they are used to guide patient treatment in clinical trials.

Optical science and engineering affect almost every aspect of our lives. Millions of miles of optical fiber carry voice and data signals around the world. Lasers are used in surgery of the retina, kidneys, and heart. New high-efficiency light sources promise dramatic reductions in electricity consumption. Night-vision equipment and satellite surveillance are changing how wars are fought. Industry uses optical methods in everything from the production of computer chips to the construction of tunnels. Harnessing Light surveys this multitude of applications, as well as the status of the optics industry and of research and education in optics, and identifies actions that could enhance the field's contributions to society and facilitate its continued technical development.