These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant. - Continues the legacy of this premier serial with quality chapters on hydrogen sulfide research authored by leaders in the field - Covers conventional and new hydrogen sulfide detection methods - Covers the pharmacological activity of hydrogen sulfide donors - Contains chapters on important topics on hydrogen sulfide modulation of cell signaling and transcriptional pathways, and the the role of hydrogen sulfide in the cardiovascular and nervous systems and in inflammation

These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant. - Continues the legacy of this premier serial with quality chapters on hydrogen sulfide research authored by leaders in the field - Covers conventional and new hydrogen sulfide detection methods - Covers the pharmacological activity of hydrogen sulfide donors - Contains chapters on important topics on hydrogen sulfide modulation of cell signaling and transcriptional pathways, and the role of hydrogen sulfide in the cardiovascular and nervous systems and in inflammation

This is the premier, single-source reference on redox biochemistry, a rapidly emerging field. This reference presents the basic principles and includes detailed chapters focusing on various aspects of five primary areas of redox biochemistry: antioxidant molecules and redox cofactors; antioxidant enzymes; redox regulation of physiological processes; pathological processes related to redox; and specialized methods. This is a go-to resource for professionals in pharmaceuticals, medicine, immunology, nutrition, and environmental fields and an excellent text for upper-level students.

Hydrogen sulfide (H2S) has emerged as an important gas signalling molecule in a series of organs/tissues, on the diseases of which it plays protective roles, such as proangiogenic effects in ischemic tissues, antiapoptotic effects in the cardiomyocytes, regularization of fatal arrhythmia in myocardial infarction, amelioration of inflammation in autoimmune diseases, modification of neuronal transmission, increase in sodium excretion from the kidney, and amelioration of insulin resistance, etc. This book focuses on the effect of hydrogen sulfide in cardiovascular system, immune system, nervous system, kidney, as well as on the metabolism of glucose and lipids and regulation of ion channels and so on. This book also provides the advances in the understanding of endogenous H2S metabolism and H2S protein targets, as well as H2S donors. It will benefit researchers in both academics and industry working on the underlying mechanism of H2S field and the future of translational medicine of H2S.

HYDROGEN SULFIDE Covers H2S interactions, methods of detection and delivery in biological environments, and a wide range of applications Research on hydrogen sulfide (H2S) spans diverse disciplines including chemistry, biology, and physiology. In recent years, new materials and approaches have been developed to deliver H2S and related reactive sulfur species in various clinical contexts. Although many biological pathways involving H2S are complex, all are governed by fundamental chemical interactions between reactive sulfur species and other molecular entities. Hydrogen Sulfide: Chemical Biology Basics, Detection Methods, Therapeutic Applications, and Case Studies provides the foundation required for understanding the fundamental chemical biology of H2S while highlighting the compound’s therapeutic potential and medicinal applications. This book covers key aspects of H2S chemical biology, including the fundamental chemistry of reactive sulfur species; the measurement, detection, and delivery of H2S in biological environments; and the therapeutic and medicinal uses of exogenous H2S delivery in various pharmacologically relevant systems. Throughout the text, editor Michael Pluth and chapter contributors discuss the opportunities and future of the multidisciplinary field. Provides approaches for delivering H2S with relevance to biological and therapeutic applications Describes complex interactions of H2S with bioinorganic complexes and reactive sulfur, nitrogen, and oxygen species Summarizes advances in available tools to detect, measure, and modulate H2S levels in biological environments, such as real-time methods for H2S fluorescence imaging in live cell and animal systems Helps readers understand known systems and make connections to new and undiscovered pathways and mechanisms of action Includes in-depth case studies of different systems in which H2S plays an important role Hydrogen Sulfide: Chemical Biology Basics, Detection Methods, Therapeutic Applications, and Case Studies is an important source of current knowledge for researchers, academics, graduate students, and industrial scientists in the fields of redox biology, hydrogen sulfide research, and medicinal chemistry of small biological molecules.

The metabolism of sulfur especially by sulfurtransferases had been intensively studied in mid 1900’s. Three enzymes, cystathionine β–synthase (CBS), cystathionine γ–lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3MST) were found to have the capacity to produce H2S in vitro. However, H2S was recognized simply as a by-product of the metabolic pathways or as a marker for evaluating the activity of enzymes rather than as a physiological active molecule. In the late 1980’s relatively high concentrations of sulfide were measured in the brain that led to the successive studies of identifying the physiological functions of H2S. Recently, the steady-state concentrations of H2S have been re-evaluated and found to be much less than that initially measured. However, despite these differences, such re-evaluations served to further confirm the existence of H2S in mammalian tissues. H2S is produced in almost every organ and plays various roles such as neuromodulation, vasodilation, insulin release, inflammation, angiogenesis and cytoprotection. The unregulated production of H2S and improper responses of target molecules are involved in the pathogenesis of various diseases. This book focuses on these topics as well as on the recent progress in the biology and the therapeutic development of this molecule.

Hydrogen Sulfide in Plant Biology: Past and Present includes 17 chapters, with topics from cross-talk and lateral root development under stress, to post-translational modifications and disease resistance. With emerging research on the different roles and applications of H2S, this title compiles the latest advances of this key signaling molecule. The development of a plant requires complex signaling of various molecules like H2S in order to achieve regulated and proper development, hence hydrogen sulfide (H2S) has emerged as an important signaling molecule that regulates nearly each and every stage of a plant's lifecycle. Edited by leading experts in the field, this is a must-read for scientists and researchers interested in plant physiology, biochemistry and ecology. - Discusses the emerging roles of H2S in plant biology - Presents the latest research from leading laboratories across the globe - Edited by a team of experts in plant signaling

This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study.

This book puts hydrogen sulfide in context with other gaseous mediators such as nitric oxide and carbon monoxide, reviews the available mechanisms for its biosynthesis and describes its physiological and pathophysiological roles in a wide variety of disease states. Hydrogen sulfide has recently been discovered to be a naturally occurring gaseous mediator in the body. Over a relatively short period of time this evanescent gas has been revealed to play key roles in a range of physiological processes including control of blood vessel caliber and hence blood pressure and in the regulation of nerve function both in the brain and the periphery. Disorders concerning the biosynthesis or activity of hydrogen sulfide may also predispose the body to disease states such as inflammation, cardiovascular and neurological disorders. Interest in this novel gas has been high in recent years and many research groups worldwide have described its individual biological effects. Moreover, medicinal chemists are beginning to synthesize novel organic molecules that release this gas at defined rates with a view to exploiting these new compounds for therapeutic benefit.