Tissue culture procedures; Preparation of subcellular fractions; Concentration and purification of viruses; Quantitative assay procedures for virus infectivity; Quantitative procedures for assay of RNA, DNA, and protein; Protein analysis; RNA analyses; Electron microscopic procedures.

The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! - Provides up-to-date techniques by experts worldwide - Presents common, step-by-step protocols in an attractive, easy-to-use fashion - Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory

Designed for graduate students and researchers in all biological and biomedical sciences, this volume brings together the basic science chapters from the two-volume Fourth Edition of Fields Virology. These 37 chapters comprise a comprehensive text and reference on the concepts and research techniques of contemporary virology and the biochemistry, molecular biology, and replication of all viruses. The first part of the book covers basic concepts of general virology and the second part focuses on specific virus families.

Fundamental Techniques in Virology

The foundational textbook on the study of virology Basic Virology, 4th Edition cements this series’ position as the leading introductory virology textbook in the world. It’s easily read style, outstanding figures, and comprehensive coverage of fundamental topics in virology all account for its immense popularity. This undergraduate-accessible book covers all the foundational topics in virology, including: The basics of virology Virological techniques Molecular biology Pathogenesis of human viral disease The 4th edition includes new information on the SARS, MERS and COVID-19 coronaviruses, hepatitis C virus, influenza virus, as well as HIV and Ebola. New virological techniques including bioinformatics and advances in viral therapies for human disease are also explored in-depth. The book also includes entirely new sections on metapneumoviruses, dengue virus, and the chikungunya virus.

"Principles of Molecular Virology, Fourth Edition" provides an essential introduction to modern virology in a clear and concise manner. It is a highly enjoyable and readable text with numerous illustrations that enhance the reader's understanding of important principles. It contains new material on virus structure, virus evolution, zoonoses, bushmeat, SARS and bioterrorism. The standard version includes a CD-ROM with Flash animations, virtual interactive tutorials and experiments, self-assessment questions, useful online resources, along with the glossary, classification of subcellular infectious agents and history of virology.

Essential Human Virology, Second Edition focuses on the structure and classification of viruses, virus transmission and virus replication strategies based upon type of viral nucleic acid. Several chapters focus on notable and recognizable viruses and the diseases caused by them, including influenza, HIV, hepatitis viruses, poliovirus, herpesviruses and emerging and dangerous viruses. Additionally, how viruses cause disease (pathogenesis) is highlighted, along with discussions on immune response to viruses, vaccines, anti-viral drugs, gene therapy, the beneficial uses of viruses, research laboratory assays and viral diagnosis assays. Fully revised and updated with new chapters on coronaviruses, nonliving infectious agents, and notable non-human viruses, the book provides students with a solid foundation in virology. - Focuses on human diseases and the cellular pathology that viruses cause - Highlights current and cutting-edge technology and associated issues - Presents real case studies and current news highlights in each chapter - Features dynamic illustrations, chapter assessment questions, key terms, and a summary of concepts, as well as an instructor website with lecture slides, a test bank and recommended activities - Updated and revised, with new chapters on coronaviruses, nonliving infectious agents, and notable non-human viruses

Fundamentals of Plant Virology is an introductory student text covering all of modern plant virology. The author, Dr. R.E.F. Matthews, has written this coursebook based on his classic and comprehensive Plant Virology, Third Edition. Four introductory chapters review properties of viruses and cells and techniques used in their study. Five chapters are devoted to current knowledge of all major plant viruses and related pathogens. Seven chapters describe biological properties such as transmission, host response, disease, ecology, control, classification, and evolution of plant viruses. A historical and future overview concludes the text. Fundamentals of Plant Virology is a carefully designed instructional format for a plant virology course. It is also an invaluable resource for students of plant pathology and plant molecular biology. - Summarizes knowledge on all aspects of plant virology - Condenses all essential material from Plant Virology 3/e - Compares basic properties of cells and viruses - Outlines principles of gene manipulation technology - Discusses serological techniques including monoclonal antibodies - Geared to student level course

Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author's more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. - Presents viruses within their family structure - Contains recommended journal articles with perspectives to put primary literature in context - Includes integrated recommended reading references within each chapter - Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor's manual, study guide, and test bank

Designed for students learning about viruses for the first time at the undergraduate or graduate level, Fundamentals of Molecular Virology is presented in a style which relates to today's students and professors. This book is also a valuable, up-to-date source of information for graduate students, postdoctoral fellows and research scientists working with viruses. Chapters contributed by prominent virologists were edited to conform to a clear and accessible style. The text provides a thorough presentation of basic and contemporary concepts in virology for a student's first exposure to the field.