This textbook is an excellent guide to microscopy for students and scientists, who use microscopy as one of their primary research and analysis tool in the laboratory. The book covers key microscopy principles and explains the various techniques such as epifluorescence microscopy, confocal/live cell imaging, SIM/light sheet microscopy, and many more. Easy-to-understand protocols provide helpful guidance for practical implementation in various commercially available imaging systems. The reader is introduced to histology and further be guided through advanced image acquisition, classification and analysis. The book is written by experienced imaging specialists from the UK, other EU countries, the US and Asia, and is based on advanced training courses for master students and PhD students. Readers are not expected to be familiar with imaging and microscopy technologies, but are introduced to the subject step by step. This textbook is indented for biomedical and medical students, as well as scientists and postdocs who want to acquire a thorough knowledge of microscopy, or gain a comprehensive overview of modern microscopy techniques used in various research laboratories and imaging facilities. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

A comprehensive biographical guide to the scientific achievements, personal lives, and struggles of women scientists from around the globe. International Women in Science: A Bibliographical Dictionary to 1950 presents the enormous contributions of women outside North America in fields ranging from aviation to computer science to zoology. It provides fascinating profiles of nearly 400 women scientists, both renowned figures like Florence Nightingale and Marie Curie and women we should know better, like Rosalind Franklin, who, along with James Watson and Francis Crick, uncovered the structure of DNA. Students and researchers will see how the lives of these remarkable women unfolded, and how they made their place in fields often stubbornly guarded by men, overcoming everything from limited education and professional opportunities, to indifference, ridicule, and cultural prejudice, to outright hostility and discrimination. Included are a number of living scientists, many of whom provide insights into their lives and scientific times. Those contributions, plus additional previously unavailable material, make this a volume of unprecedented scope and richness.

In the tumultuous, ever-shifting terrain of the digital age, this groundbreaking introductory book offers the strength and stability of the fundamental aesthetic and technical building blocks necessary to create visually stimulating and thought-provoking digitally based photographs.

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy