Essentials Of Single Cell Analysis
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Author |
: Fan-Gang Tseng |
Publisher |
: Springer |
Total Pages |
: 415 |
Release |
: 2016-01-21 |
ISBN-10 |
: 9783662491188 |
ISBN-13 |
: 3662491184 |
Rating |
: 4/5 (88 Downloads) |
Synopsis Essentials of Single-Cell Analysis by : Fan-Gang Tseng
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Author |
: John Maclane |
Publisher |
: Createspace Independent Publishing Platform |
Total Pages |
: 386 |
Release |
: 2017-06 |
ISBN-10 |
: 1548019216 |
ISBN-13 |
: 9781548019211 |
Rating |
: 4/5 (16 Downloads) |
Synopsis Essentials of Single-Cell Analysis by : John Maclane
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations.
Author |
: Guo-Cheng Yuan |
Publisher |
: Humana Press |
Total Pages |
: 271 |
Release |
: 2019-02-14 |
ISBN-10 |
: 149399056X |
ISBN-13 |
: 9781493990566 |
Rating |
: 4/5 (6X Downloads) |
Synopsis Computational Methods for Single-Cell Data Analysis by : Guo-Cheng Yuan
This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis.
Author |
: Harris G. Fienberg |
Publisher |
: Springer |
Total Pages |
: 224 |
Release |
: 2014-04-22 |
ISBN-10 |
: 9783642548277 |
ISBN-13 |
: 364254827X |
Rating |
: 4/5 (77 Downloads) |
Synopsis High-Dimensional Single Cell Analysis by : Harris G. Fienberg
This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers.
Author |
: Jin-Ming Lin |
Publisher |
: Springer Nature |
Total Pages |
: 263 |
Release |
: 2019-08-28 |
ISBN-10 |
: 9789813297296 |
ISBN-13 |
: 9813297298 |
Rating |
: 4/5 (96 Downloads) |
Synopsis Microfluidics for Single-Cell Analysis by : Jin-Ming Lin
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Author |
: J. Paul Robinson |
Publisher |
: Springer |
Total Pages |
: 277 |
Release |
: 2017-04-18 |
ISBN-10 |
: 9789811044991 |
ISBN-13 |
: 9811044996 |
Rating |
: 4/5 (91 Downloads) |
Synopsis Single Cell Analysis by : J. Paul Robinson
This book highlights the current state of the art in single cell analysis, an area that involves many fields of science – from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research. This book brings together an eclectic group of current applications, all of which have a significant impact on our current state of knowledge. The authors of these chapters are all pioneering researchers in the field of single cell analysis. The book will not only appeal to those readers more focused on clinical applications, but also those interested in highly technical aspects of the technologies. All of the technologies identified utilize unique applications of photon detection systems.
Author |
: Gary Durack |
Publisher |
: John Wiley & Sons |
Total Pages |
: 375 |
Release |
: 2004-03-24 |
ISBN-10 |
: 9780471461005 |
ISBN-13 |
: 0471461008 |
Rating |
: 4/5 (05 Downloads) |
Synopsis Emerging Tools for Single-Cell Analysis by : Gary Durack
The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies. Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis. By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,
Author |
: Xiangdong Wang |
Publisher |
: Springer |
Total Pages |
: 184 |
Release |
: 2015-03-27 |
ISBN-10 |
: 9789401797535 |
ISBN-13 |
: 9401797536 |
Rating |
: 4/5 (35 Downloads) |
Synopsis Single Cell Sequencing and Systems Immunology by : Xiangdong Wang
The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.
Author |
: Miodrag Gužvić |
Publisher |
: Springer Nature |
Total Pages |
: 263 |
Release |
: 2024-02-10 |
ISBN-10 |
: 9781071636213 |
ISBN-13 |
: 1071636219 |
Rating |
: 4/5 (13 Downloads) |
Synopsis Single Cell Analysis by : Miodrag Gužvić
This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Single Cell Analysis: Methods and Protocols is a valuable tool for any researcher interested in learning more about this important and developing field.
Author |
: Fan-Gang Tseng |
Publisher |
: MDPI |
Total Pages |
: 237 |
Release |
: 2018-10-01 |
ISBN-10 |
: 9783038421931 |
ISBN-13 |
: 3038421936 |
Rating |
: 4/5 (31 Downloads) |
Synopsis Single Cell Analysis in Biotechnology and Systems Biology by : Fan-Gang Tseng
This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS