Dynamics Of Nuclear Architecture Investigated By Live Cell Microscopy And Quantitative 4 D Reconstruction
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Author |
: Daniel Gerlich |
Publisher |
: |
Total Pages |
: 68 |
Release |
: 2002 |
ISBN-10 |
: OCLC:76380322 |
ISBN-13 |
: |
Rating |
: 4/5 (22 Downloads) |
Synopsis Dynamics of Nuclear Architecture Investigated by Live Cell Microscopy and Quantitative 4-D Reconstruction by : Daniel Gerlich
Author |
: Christophe Lavelle |
Publisher |
: Academic Press |
Total Pages |
: 620 |
Release |
: 2017-10-27 |
ISBN-10 |
: 9780128035030 |
ISBN-13 |
: 012803503X |
Rating |
: 4/5 (30 Downloads) |
Synopsis Nuclear Architecture and Dynamics by : Christophe Lavelle
Nuclear Architecture and Dynamics provides a definitive resource for (bio)physicists and molecular and cellular biologists whose research involves an understanding of the organization of the genome and the mechanisms of its proper reading, maintenance, and replication by the cell. This book brings together the biochemical and physical characteristics of genome organization, providing a relevant framework in which to interpret the control of gene expression and cell differentiation. It includes work from a group of international experts, including biologists, physicists, mathematicians, and bioinformaticians who have come together for a comprehensive presentation of the current developments in the nuclear dynamics and architecture field. The book provides the uninitiated with an entry point to a highly dynamic, but complex issue, and the expert with an opportunity to have a fresh look at the viewpoints advocated by researchers from different disciplines. - Highlights the link between the (bio)chemistry and the (bio)physics of chromatin - Deciphers the complex interplay between numerous biochemical factors at task in the nucleus and the physical state of chromatin - Provides a collective view of the field by a large, diverse group of authors with both physics and biology backgrounds
Author |
: |
Publisher |
: |
Total Pages |
: |
Release |
: 2003 |
ISBN-10 |
: OCLC:671471109 |
ISBN-13 |
: |
Rating |
: 4/5 (09 Downloads) |
Synopsis Modelling Nuclear Body Dynamics in Living Cells by 4-D Microscopy, Image Analysis and Simulation by :
The work presented here demonstrates rules of and validates models for nuclear body (NB) dynamics. Simulation tools developed in the course of this work can be used in future work to generate hypotheses about related aspects of nuclear architecture. Initially I examined the mobility of vimentin nuclear bodies bodies (VNB) in interphase by single particle tracking and analysis of fluorescence images from 4-D confocal laser scanning microscopy (CLSM). These synthetic nuclear bodies are observed in cells transfected with labelled nuclear-targeted Xenopus laevis vimentin. Analysis shows that VNBs undergo anomalous diffusion in the nuclei, independent of metabolic energy. Individual bodies display either one of the three modes of diffusion -- directed, restricted or simple. The consistency of modes and magnitudes of diffusion constants between VNBs and bona fide nuclear bodies points to a generic mechanism that mediates and regulates the mobility of nuclear bodies. Since the results of diffusion analysis of VNBs did not agree with a simple diffusion model, I tested the alternative interchromosomal domain (ICD) compartment model. The ICD model predicts that in interphase cell nuclei, individual decompacted chromosomes do not intermingle, but are separated by a significant interchromatin space forming a network of channels. These networks could affect the mobility of nuclear bodies. Monte Carlo simulations that predict the effects of channels and other obstructions on NB diffusion were tested, but they could not explain deviation from ideal behaviour. Fitting an empirical model of `critical diffusion' produced similar results. Therefore the ICD model as a purely obstructing network of channels needs modification, to possibly include binding. To examine the role of chromatin density in intra-nuclear diffusion, I employed multidimensional fluorescence recovery after photobleaching (FRAP) in living cells. The influence of chromatin density on diffusive mobility of the nuclear.
Author |
: Niall M. Adams |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 214 |
Release |
: 2010-10-17 |
ISBN-10 |
: 9789048198993 |
ISBN-13 |
: 9048198992 |
Rating |
: 4/5 (93 Downloads) |
Synopsis Advances in Nuclear Architecture by : Niall M. Adams
This book provides a snapshot of the state-of-the art in the study of mammalian cell nuclear architecture, and features a diverse range of chapters written by top researchers. A key aspect is an emphasis on precise and repeatable quantitative analysis and simulation in addition to the more familiar biological perspective. The fusion of such material frames the future of the discipline. Quantitative contributions stress reproducible and robust 3D analysis, using a variety of tools ranging from point pattern analysis to shape registration methods. Biological insights include the role of nuclear subdomains in cancer, nuclear molecular motors, and a holistic view of gene transcription.
Author |
: K. Nagata |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 285 |
Release |
: 2007-12-03 |
ISBN-10 |
: 9784431301301 |
ISBN-13 |
: 4431301305 |
Rating |
: 4/5 (01 Downloads) |
Synopsis Nuclear Dynamics by : K. Nagata
The dynamics of nuclear structures described in this book furnish the basis for a comprehensive understanding of how the higher-order organization and function of the nucleus is established and how it correlates with the expression of a variety of vital activities such as cell proliferation and differentiation. The resulting volume creates an invaluable source of reference for researchers in the field.
Author |
: Chaitanya A. Athale |
Publisher |
: |
Total Pages |
: 0 |
Release |
: 2003 |
ISBN-10 |
: OCLC:723230209 |
ISBN-13 |
: |
Rating |
: 4/5 (09 Downloads) |
Synopsis Modelling Nuclear Body Dynamics in Living Cells by 4-D Microscopy, Image Analysis and Simulation by : Chaitanya A. Athale
Author |
: |
Publisher |
: |
Total Pages |
: 700 |
Release |
: 2002 |
ISBN-10 |
: UOM:39015056644126 |
ISBN-13 |
: |
Rating |
: 4/5 (26 Downloads) |
Synopsis Molecular Biology of the Cell by :
MBC online publishes papers that describe and interpret results of original research conserning the molecular aspects of cell structure and function.
Author |
: Hilmar Strickfaden |
Publisher |
: |
Total Pages |
: 0 |
Release |
: 2010 |
ISBN-10 |
: OCLC:643113515 |
ISBN-13 |
: |
Rating |
: 4/5 (15 Downloads) |
Synopsis Nuclear architecture explored by live-cell fluorescence microscopy using laser and ion microbeam irradiation by : Hilmar Strickfaden
Author |
: Vincent RĂ©camier |
Publisher |
: |
Total Pages |
: 0 |
Release |
: 2013 |
ISBN-10 |
: OCLC:879573841 |
ISBN-13 |
: |
Rating |
: 4/5 (41 Downloads) |
Synopsis Single Particle Imaging in the Cell Nucleus by : Vincent RĂ©camier
The cell nucleus is a chemical reactor. Nuclear components interact with each other to express genes, duplicate the chromosomes for cell division, and protect DNA from alteration. These reactions are regulated along the cell cycle and in response to stress. One of the fundamental nuclear processes, transcription, enables the production of a messenger RNA from a template DNA sequence. While mandatory for the cell, transcription nevertheless may involve a very small number of molecules. Indeed, a single gene would have only few copies in the genome. During my PhD, I studied nuclear processes in human cells nuclei at the single molecule level with novel imaging techniques. I developed new statistical tools to quantify nuclear components movement that revealed a dynamic nuclear architecture. Since the 90s, simple methods have been developed for the observation of single molecules in the cell. These experiments can be conducted in an ordinary inverted microscope. We used these methods to monitor nuclear molecules called transcription factors (TF) that regulate transcription. From TF dynamics, we concluded that nuclear exploration by transcription factors is regulated by their chemical interactions with partners. The organization of the components of the nucleus guide transcription factors in their search of a gene. As an example of this organization, we then studied chromatin, the de-condensed form of nuclear DNA, proving that it displays the characteristics of a self-organized fractal structure. This structure changes in response to cellular fate and stress. In yeast, we showed that the interminglement of chromatin constrained DNA locus movement in a reptation regime. All these results show the interdependence of the structure of the nucleus and of its chemical reactions. With combination of realistic modeling and high resolution microscopy, we have enlightened the specificity of the nucleus as a chemical reactor. This thesis has also enabled the development of accurate methods for the statistical analysis of single molecule data.
Author |
: Yan Shi |
Publisher |
: Frontiers Media SA |
Total Pages |
: 116 |
Release |
: 2021-11-15 |
ISBN-10 |
: 9782889716340 |
ISBN-13 |
: 2889716341 |
Rating |
: 4/5 (40 Downloads) |
Synopsis The Role of Biomembranes and Biophysics in Immune Cell Signaling by : Yan Shi