This book is part of the Plant Pathology in the 21st Century Series, started in the occasion of the IX International Congress of Plant Pathology, Torino, 2008. In conjuction with the Xth International Congress of Plant Pathology, held in Beijing in August 2013. Although deriving from a Congress, the book will not have the format of traditional Proceedings, but will be organized as a resource book. It will be based on invited lectures presented at the Congress as well as by other chapters selected by the editors among offered papers. This book will cover a topic very important in the field of plant pathology, dealing with detection and diagnostics. This field of research is continuously moving forwards, due to innovation in techniques. The application of new detection and diagnostic technologies are relevant to many applied fields in agriculture. The different chapters will provide a very complete figure of the topic, from general and basic aspects to practical aspects.

Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.

Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.

Plant diseases play an important role on our daily lives. Most of plant diseases are visible and are caused by biotic and/or abiotic factors. Symptoms are usually the results of a morphological change, alteration or damage to plant tissue and/or cells due to an interference of the plant’s metabolism. All basic structures of vascular plants are subject to attack by pathogens. The failure in accurate disease diagnosis and management may lead to huge losses in plant production and related commodities, which causes nutritional food scarcity. Typically, the appearance of a biotic symptom will indicate the relatively late stage of an infection and/or colonization of a pathogen. Expert detection, accurate diagnosis, and timely management play a significant role in keeping plants free from pathogens. In this book expert scholars share their research knowledge and key literature which are vital toward the diagnosis of plant diseases across the globe, addressing traditional plant pathology techniques, as well as advanced molecular diagnostic approach.

The main theme of the book is sustainable disease management in a European context. Some of the questions addressed are: How does society benefit from plant pathology research? How can new molecular approaches solve relevant problems in disease management? What other fields can we exploit in plant pathology research? What challenges are associated with free trade across the new borders? How can we contribute to solving problems of developing countries? How does plant pathology contribute to food quality and safety? How does globalization/internationalization affect teaching and extension in plant pathology?

This book is a compilation of the most challenging and significant chapters on the diagnosis and management of important bacterial, fungal, viral, viroid, phytoplasma, non parasitic diseases and various physiological disorders, in various crops. The chapters have been contributed by eminent plant pathologists, having wide experience of teaching and research on various crops with different types of diseases, which cause great economic losses. The book would be very useful for students, teachers and researchers of plant pathology. This book highlights recent advances made in the development of new types of resistance in host plants and alternative strategies for managing plant diseases to improve food quality and reduce the negative public health impact associated with plant diseases. Having entered into 21st century advancements in the Diagnosis of Plant Pathogens and Plant Disease Management need to be closely examined and adequately applied, so that newer challenges facing plant pathology could be adequately addressed in attaining food security for the growing population. Substantial advancements have been made in terms of expanding knowledge base of the biology of plant-microbial interactions, disease management strategies and application and practice of Plant Pathology. Application of molecular biology in Plant Pathology has greatly improved our ability to detect plant pathogens and in increasing our understanding, their ecology and epidemiology. Similarly, new technologies and resources have been evolved for the development of sustainable crop protection systems by different control strategies against various pests and pathogens that are important components of the integrated pest management programme. Natural products and chemical compounds discovered as a result of basic research and molecular mechanisms of pathogenesis have led to the development of “biorational” pesticides. Biological control has been found to be the most significant approach to plant health management during the twentieth century and promises using modern biotechnology, to be even more significant in the twenty-first century.

The diagnosis of plant disease; The microscope; The autoclave; The preparation of media for fungal and bacterial growth; Detection of fungal pathogens in infected plant tissues; Detection of bacterial pathogens in infected tissues; Koch's postulates; The diagnosis of a nematode prblem; Viruses and plant virus diseases; Mycoplasma as agents of plant disease.

This substantially updated edition now in full colour provides key techniques used when working with fungal and fungal-like plant pathogens. As a practical manual it also deals with disease recognition, detection and identification of fungi, plus methods to characterise and curate fungi and handle them under quarantine and quality assurance systems. Fungal Plant Pathogens: Applied Techniques, 2nd edition provides a valuable guide to investigating fungal plant diseases and interpreting laboratory findings for postgraduate and advanced undergraduate students, extension plant pathologists, consultants and advisers in agriculture, forestry and horticulture, and the food supply chain.

Handbook of Plant Disease Identification and Management presents the fundamentals of plant diseases identification based on symptomology and management focusing mainly on integrated pest management approach. It discusses a variety of techniques for the diagnosis of crop disease, losses due to crop diseases, and theories behind disease management. It describes how society is constraining the possibilities for management of crop diseases by changing the environment; biologically controlling crop diseases; and the epidemiologic and genetic concepts of managing host genes. This book discusses managing diseases through diverse chemical, biological, and physical methods. It highlights climatic factors affecting crops by creating favorable condition for most of the diseases. This book serves as a complete guide for growers, researchers, and graduate students to understand basics of plant disease identification. It explains the disease cycle for respective crops with favorable conditions promoting disease development. It intends to aid growers in managing diseases and help scientists with future research.

This book is the second volume on this topic within the series. With unique properties, nanomaterials are rapidly finding novel applications in many fields such as food, medicine, agriculture and pollution. Such applications include to treat cancer, nanosensors to detect food contamination, nanomaterials for food packaging, nanoencapsulation to preserve nutraceuticals, and nanofertilisers for advanced agriculture. After an introductory chapter on property rights of nanomaterials, readers will discover the applications of nanotechnology in food, health, environment, ecotoxicology and agriculture.