Chromatin Protocols
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Author |
: Remus T. Dame |
Publisher |
: Springer Nature |
Total Pages |
: 654 |
Release |
: |
ISBN-10 |
: 9781071639306 |
ISBN-13 |
: 1071639307 |
Rating |
: 4/5 (06 Downloads) |
Synopsis Bacterial Chromatin by : Remus T. Dame
Author |
: Peter B. Becker |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 525 |
Release |
: 2008-02-03 |
ISBN-10 |
: 9781592596812 |
ISBN-13 |
: 1592596819 |
Rating |
: 4/5 (12 Downloads) |
Synopsis Chromatin Protocols by : Peter B. Becker
More than 40 years after the discovery of the nucleosome as the fun- mental unit of chromatin, the multifaceted problem of how variations in ch- matin structure affect the activity of the eukaryotic genome has not been solved. However, during the past few years research on chromatin structure and fu- tion has gained considerable momentum, and impressive progress has been made at the level of concept development as well as filling in crucial detail. The structure of the nucleosome has been visualized at unprecedented reso- tion. Powerful multisubunit enzymes have been identified that alter histone/ DNA interactions in ways that expose regulatory sequences to factors initi- ing and regulating such nuclear processes as transcription. Though the imp- tance of posttranslational modifications of histones, notably their acetylation, has long been known, the finding that a number of bona fide regulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical properties and varying degrees of DNA condensation. Perhaps the most important conclusion from a large number of studies is a fresh appreciation of the dynamic nature of chromatin structure, the built-in flexibility providing the basis for regulation.
Author |
: Ulf Andersson Vang Ørom |
Publisher |
: Humana |
Total Pages |
: 0 |
Release |
: 2020-07-18 |
ISBN-10 |
: 1071606794 |
ISBN-13 |
: 9781071606797 |
Rating |
: 4/5 (94 Downloads) |
Synopsis RNA-Chromatin Interactions by : Ulf Andersson Vang Ørom
This volume focuses on RNAs interacting with chromatin and their function. Chapters guide readers through transcription, splicing, non-coding RNA function and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Chromatin Interactions: Methods and Protocols aims to be a starting-point to expand researchers experimental approaches towards the numerous outstanding questions in this new and expanding field.
Author |
: Philippe Collas |
Publisher |
: Methods in Molecular Biology |
Total Pages |
: 290 |
Release |
: 2009-08-25 |
ISBN-10 |
: UOM:39015075683279 |
ISBN-13 |
: |
Rating |
: 4/5 (79 Downloads) |
Synopsis Chromatin Immunoprecipitation Assays by : Philippe Collas
In this book, researchers deeply involved in the development and improvement of chromatin immunoprecipitation assays (ChIP) provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects.
Author |
: |
Publisher |
: |
Total Pages |
: |
Release |
: |
ISBN-10 |
: 047150338X |
ISBN-13 |
: 9780471503385 |
Rating |
: 4/5 (8X Downloads) |
Synopsis Current Protocols in Molecular Biology by :
Author |
: Beatrice Bodega |
Publisher |
: Humana |
Total Pages |
: 322 |
Release |
: 2020-10-29 |
ISBN-10 |
: 1071606638 |
ISBN-13 |
: 9781071606636 |
Rating |
: 4/5 (38 Downloads) |
Synopsis Capturing Chromosome Conformation by : Beatrice Bodega
This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.
Author |
: Mark C. Leake |
Publisher |
: Humana |
Total Pages |
: 0 |
Release |
: 2016-06-10 |
ISBN-10 |
: 1493936298 |
ISBN-13 |
: 9781493936298 |
Rating |
: 4/5 (98 Downloads) |
Synopsis Chromosome Architecture by : Mark C. Leake
This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author |
: Guillermo A. Orsi |
Publisher |
: Humana |
Total Pages |
: 359 |
Release |
: 2019-08-17 |
ISBN-10 |
: 1493993585 |
ISBN-13 |
: 9781493993581 |
Rating |
: 4/5 (85 Downloads) |
Synopsis Histone Variants by : Guillermo A. Orsi
This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information. Its four sections cover experimental approaches to probe the biochemistry of histone variants and variant nucleosomes; their dynamics throughout the cell cycle and at specific genomic locations; their functional impact on chromosome organization and genome stability; and their importance in development, disease and evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, illustrations on the expected results, and tips on troubleshooting and avoiding known pitfalls. This anthology brings together contributions from many of the most highly recognized investigators in this community. Cutting-edge and thorough, Histone Variants: Methods and Protocols is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field.
Author |
: Daryl S. Henderson |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 498 |
Release |
: 2008-02-03 |
ISBN-10 |
: 9781592599738 |
ISBN-13 |
: 1592599737 |
Rating |
: 4/5 (38 Downloads) |
Synopsis DNA Repair Protocols by : Daryl S. Henderson
The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book’s thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.
Author |
: Trygve O. Tollefsbol |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 301 |
Release |
: 2008-02-02 |
ISBN-10 |
: 9781592598281 |
ISBN-13 |
: 1592598285 |
Rating |
: 4/5 (81 Downloads) |
Synopsis Epigenetics Protocols by : Trygve O. Tollefsbol
The field of epigenetics has grown exponentially in the past decade, and a steady flow of exciting discoveries in this area has served to move it to the forefront of molecular biology. Although epigenetics may previously have been considered a peripheral science, recent advances have shown considerable progress in unraveling the many mysteries of nontraditional genetic processes. Given the fast pace of epigenetic discoveries and the groundbreaking nature of these developments, a thorough treatment of the methods in the area seems timely and appropriate and is the goal of Epigenetics Protocols. The scope of epigenetics is vast, and an exhaustive analysis of all of the techniques employed by investigators would be unrealistic. However, this TM volume of Methods in Molecular Biology covers three main areas that should be of greatest interest to epigenetics investigators: (1) techniques related to analysis of chromatin remodeling, such as histone acetylation and methylation; (2) methods in newly developed and especially promising areas of epigenetics such as telomere position effects, quantitative epigenetics, and ADP ribosylation; and (3) an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure.