Cellular Electron Microscopy
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Author |
: J. Richard McIntosh |
Publisher |
: Elsevier |
Total Pages |
: 878 |
Release |
: 2011-09-02 |
ISBN-10 |
: 9780080475035 |
ISBN-13 |
: 0080475035 |
Rating |
: 4/5 (35 Downloads) |
Synopsis Cellular Electron Microscopy by : J. Richard McIntosh
Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. - Updates the numerous technological innovations that have improved the capabilities of electron microscopy - Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging - Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going
Author |
: |
Publisher |
: Academic Press |
Total Pages |
: 562 |
Release |
: 2008-10-22 |
ISBN-10 |
: 9780080888163 |
ISBN-13 |
: 008088816X |
Rating |
: 4/5 (63 Downloads) |
Synopsis Introduction to Electron Microscopy for Biologists by :
This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions
Author |
: Thomas Muller-Reichert |
Publisher |
: Academic Press |
Total Pages |
: 466 |
Release |
: 2012-08-10 |
ISBN-10 |
: 9780124160262 |
ISBN-13 |
: 0124160263 |
Rating |
: 4/5 (62 Downloads) |
Synopsis Correlative Light and Electron MIcroscopy by : Thomas Muller-Reichert
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
Author |
: Heide Schatten |
Publisher |
: Cambridge University Press |
Total Pages |
: 275 |
Release |
: 2013 |
ISBN-10 |
: 9780521195997 |
ISBN-13 |
: 0521195993 |
Rating |
: 4/5 (97 Downloads) |
Synopsis Scanning Electron Microscopy for the Life Sciences by : Heide Schatten
A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.
Author |
: |
Publisher |
: |
Total Pages |
: 0 |
Release |
: 2002 |
ISBN-10 |
: 0815332181 |
ISBN-13 |
: 9780815332183 |
Rating |
: 4/5 (81 Downloads) |
Synopsis Molecular Biology of the Cell by :
Author |
: Eric Hanssen |
Publisher |
: Springer |
Total Pages |
: 339 |
Release |
: 2017-12-30 |
ISBN-10 |
: 9783319689975 |
ISBN-13 |
: 3319689975 |
Rating |
: 4/5 (75 Downloads) |
Synopsis Cellular Imaging by : Eric Hanssen
This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Author |
: Roland A. Fleck |
Publisher |
: John Wiley & Sons |
Total Pages |
: 741 |
Release |
: 2019-04-29 |
ISBN-10 |
: 9781118654064 |
ISBN-13 |
: 1118654064 |
Rating |
: 4/5 (64 Downloads) |
Synopsis Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by : Roland A. Fleck
The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Author |
: Frances M. Ross |
Publisher |
: Cambridge University Press |
Total Pages |
: 1052 |
Release |
: 2016-11-24 |
ISBN-10 |
: 9781316883204 |
ISBN-13 |
: 1316883205 |
Rating |
: 4/5 (04 Downloads) |
Synopsis Liquid Cell Electron Microscopy by : Frances M. Ross
The first book on the topic, with each chapter written by pioneers in the field, this essential resource details the fundamental theory, applications, and future developments of liquid cell electron microscopy. This book describes the techniques that have been developed to image liquids in both transmission and scanning electron microscopes, including general strategies for examining liquids, closed and open cell electron microscopy, experimental design, resolution, and electron beam effects. A wealth of practical guidance is provided, and applications are described in areas such as electrochemistry, corrosion and batteries, nanocrystal growth, biomineralization, biomaterials and biological processes, beam-induced processing, and fluid physics. The book also looks ahead to the future development of the technique, discussing technical advances that will enable higher resolution, analytical microscopy, and even holography of liquid samples. This is essential reading for researchers and practitioners alike.
Author |
: Yoshiyuki Kubota |
Publisher |
: Frontiers Media SA |
Total Pages |
: 220 |
Release |
: 2019-12-30 |
ISBN-10 |
: 9782889632572 |
ISBN-13 |
: 2889632571 |
Rating |
: 4/5 (72 Downloads) |
Synopsis Electron-Microscopy-Based Tools for Imaging Cellular Circuits and Organisms by : Yoshiyuki Kubota
Author |
: Peter G. Toner |
Publisher |
: |
Total Pages |
: 282 |
Release |
: 1971 |
ISBN-10 |
: UCAL:B3760673 |
ISBN-13 |
: |
Rating |
: 4/5 (73 Downloads) |
Synopsis Cell Structure by : Peter G. Toner