Biological Specimen Preparation For Transmission Electron Microscopy
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Author |
: Audrey M. Glauert |
Publisher |
: Princeton University Press |
Total Pages |
: 349 |
Release |
: 2014-07-14 |
ISBN-10 |
: 9781400865024 |
ISBN-13 |
: 1400865026 |
Rating |
: 4/5 (24 Downloads) |
Synopsis Biological Specimen Preparation for Transmission Electron Microscopy by : Audrey M. Glauert
This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Author |
: Jeanne Ayache |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 338 |
Release |
: 2010-06-08 |
ISBN-10 |
: 1441959742 |
ISBN-13 |
: 9781441959744 |
Rating |
: 4/5 (42 Downloads) |
Synopsis Sample Preparation Handbook for Transmission Electron Microscopy by : Jeanne Ayache
Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Author |
: Jeanne Ayache |
Publisher |
: Springer |
Total Pages |
: 250 |
Release |
: 2010-07-08 |
ISBN-10 |
: 0387981810 |
ISBN-13 |
: 9780387981819 |
Rating |
: 4/5 (10 Downloads) |
Synopsis Sample Preparation Handbook for Transmission Electron Microscopy by : Jeanne Ayache
Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Author |
: Jeanne Ayache |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 267 |
Release |
: 2010-07-03 |
ISBN-10 |
: 9780387981826 |
ISBN-13 |
: 0387981829 |
Rating |
: 4/5 (26 Downloads) |
Synopsis Sample Preparation Handbook for Transmission Electron Microscopy by : Jeanne Ayache
Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Author |
: Jeanne Ayache |
Publisher |
: Springer |
Total Pages |
: 0 |
Release |
: 2010-07-09 |
ISBN-10 |
: 1441960872 |
ISBN-13 |
: 9781441960870 |
Rating |
: 4/5 (72 Downloads) |
Synopsis Sample Preparation Handbook for Transmission Electron Microscopy by : Jeanne Ayache
This two-volume Handbook is a comprehensive guide to sample preparation for the transmission electron microscope. The first volume covers general theoretical and practical aspects of the methodologies used for TEM analysis and observation of any sample. The information will help you to choose the best preparative technique for your application taking into account material types, structures, and their properties. Physical properties, material classification, and microstructures are considered together with a thorough description of the physics and chemistry of sample preparation and the main artifacts brought about by mechanical, physical and chemical methods, principles which are also applicable to sample preparation for the SEM, AFM etc.. Also included is a discussion of how to combine techniques for complex sample analysis and to obtain a TEM thin slice. Sample Preparation Handbook for Transmission Electron Microscopy: Methodology will guide you through the most current techniques for successful sample preparation in all fields from materials science to biology. The second volume, Sample Preparation Handbook for Transmission Electron Microscopy: Techniques, describes 14 different preparation techniques, including 22 detailed protocols for preparing thin slices for TEM analysis. Compatibility and pre-treatments are also discussed. Experimental conditions and guidelines, options and variations, advantages and constraints, technical hints from the authors’ years of experience, common artifacts, and theoretical issues are all considered. Particular attention is given to the type of material, conditioning, compatible analysis of a given preparation, and risks. This practical and authoritative reference companion deserves a place on the bench in every TEM lab. Key Features of the Handbook: Combines all of the latest techniques for the preparation of mineral to biological samples Compares techniques in terms of their application areas, limitations, artifacts, and types of analysis (macroscopic, atomic, or molecular level) Describes physical characteristics, chemistry, structure/texture, and orientation properties of materials in relation to the most appropriate type of TEM analysis Links to a complementary interactive database website which is available to scientists worldwide* Written by authors with 100 years of combined experience in electron microscopy *http://temsamprep.in2p3.fr/
Author |
: PJ Goodhew |
Publisher |
: Garland Science |
Total Pages |
: 50 |
Release |
: 2020-11-25 |
ISBN-10 |
: 9781000142761 |
ISBN-13 |
: 1000142760 |
Rating |
: 4/5 (61 Downloads) |
Synopsis Specimen Preparation for Transmission Electron Microscopy of Materials by : PJ Goodhew
Details the essential practical steps which must precede microscopy. Methods for preparing sheet or disc specimens and final thinning techniques are described with reference to practical problems. The book also covers methods for mounting specimens in the
Author |
: Audrey M. Glauert |
Publisher |
: |
Total Pages |
: 326 |
Release |
: 1998 |
ISBN-10 |
: 0720442508 |
ISBN-13 |
: 9780720442502 |
Rating |
: 4/5 (08 Downloads) |
Synopsis Practical Methods in Electron Microscopy by : Audrey M. Glauert
Author |
: Audrey M. Glauert |
Publisher |
: |
Total Pages |
: |
Release |
: 1998 |
ISBN-10 |
: 0444104046 |
ISBN-13 |
: 9780444104045 |
Rating |
: 4/5 (46 Downloads) |
Synopsis Practical Methods in Electron Microscopy: Biological specimen preparation for transmission electron microscopy by : Audrey M. Glauert
Author |
: Peter J. Goodhew |
Publisher |
: Garland Science |
Total Pages |
: 56 |
Release |
: 1984 |
ISBN-10 |
: UCAL:B4564362 |
ISBN-13 |
: |
Rating |
: 4/5 (62 Downloads) |
Synopsis Specimen Preparation for Transmission Electron Microscopy of Materials by : Peter J. Goodhew
Details the essential practical steps which must precede microscopy. Methods for preparing sheet or disc specimens and final thinning techniques are described with reference to practical problems. The book also covers methods for mounting specimens in the
Author |
: James Pawley |
Publisher |
: Springer Science & Business Media |
Total Pages |
: 323 |
Release |
: 2007-12-03 |
ISBN-10 |
: 9780387729725 |
ISBN-13 |
: 0387729720 |
Rating |
: 4/5 (25 Downloads) |
Synopsis Biological Low-Voltage Scanning Electron Microscopy by : James Pawley
Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.