Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. - Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding - Offers many details not present in other protocol books - Describes relevant controls for each technique and what those controls mean - Chapters include references (key articles, books, protocols) for additional study - Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Ranaviruses and other viruses within the family Iridoviridae, infect a wide range of ecologically and commercially important ectothermic vertebrates, i.e., bony fish, amphibians, and reptiles, and invertebrates, including agricultural and medical pests and cultured shrimp and crayfish, and are responsible for considerable morbidity and mortality. Understanding the impact of these various agents on diverse host species requires the combined efforts of ecologists, veterinarians, pathologists, comparative immunologists and molecular virologists. Unfortunately, investigators involved in these studies often work in discipline-specific silos that preclude interaction with others whose insights and approaches are required to comprehensively address problems related to ranavirus/iridovirus disease. Our intent here is to breakdown these silos and provide a forum where diverse researchers with a common interest in ranavirus/iridovirus biology can profitably interact. As a colleague once quipped, “Three people make a genius.” We are hoping to do something along those lines by presenting a collection of research articles dealing with issues of anti-viral immunity, identification of a potentially novel viral genus exemplified by erythrocytic necrosis virus, viral inhibition of innate immunity, identification of novel hosts for lymphocystivirus and invertebrate iridoviruses, and modelling studies of ranavirus transmission. Collectively these and others will exemplify the breadth of ongoing studies focused on this virus family.

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as well as the use of animal models and PARP1 inhibitor design and testing, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Third Edition presents essential new and classical methods for studying the pADPr-pathway.

This edited book provides an overview of omics technologies and methods for integration across multiple omics layers used in the plant disease diagnosis and developing management strategies. The book concentrates on the prevalence of soil-borne disease management in various important crops with use of different strategies, including host resistance and biological control etc. The special focus is on the resolving practical problems encountered after the resistance development in the pathogens against several chemical pesticides. Further, special attention is given to the emergence of new diseases or the re-emergence of old ones on several crops, and on the results and problems encountered by using microbial inoculants, biofumigation and other non-chemical control methods. This book has 18 contributory chapters from the eminent experts in the field of plant pathology, microbiology and biotechnology working on different aspects of soil-borne diseases of important agricultural crops. This edited volume is of interest and useful to researchers in plant pathology, agriculture sciences, plant genomics ecology, policy makers, also it is a valuable source of reference to the relevant researchers and students globally.

This book reviews current immunotherapeutic strategies for gastrointestinal (GI) malignancies, including immune composition, immune checkpoint inhibitors, cell therapy, and peptide vaccines used to protect against esophageal, gastric, hepato-biliary, pancreatic and colorectal cancers. It also discusses the current challenges of using immunotherapy for the treatment of gastrointestinal malignancies. The book reviews highly sensitive and specific immunomarkers for the detection of GI malignancies, and examines therapeutic vaccines and the major cytokines involved in GI immunotherapy, as well as their basic biology and clinical applications. In closing, the book explores various aspects of computational biology for the detection and treatment of GI malignancies.

This book gathers selected papers from the First International Symposium on Bioinformatics and Biomedicine. Issues related to medicine and health care constitute one of the grand challenges faces by the mankind, and this naturally implies a growing interest in these problems among both researchers and scholars, politicians and policymakers, as well as economist. The present values which gather selected papers from the First International Symposium on Bioinformatics and Biomedicine (BioInfoMed’2020) is a recent response to this interests. In the subsequent sections and chapters, it covers a multitude of various topics related to bioinformatics, biomedicine, bioenginering, as well as a broadly perceives healthcare. Issues related to decision making in bioinformatics, biomedicine and health care, mathematical modelling in biomedicine and health care, artificial intelligence in biomedicine and health care, uncertainty and imprecision, notably intuitionistic fuzzy sets with applications in bioinformatics and biomedicine, biomedical approaches and applications, biomedical imaging and image processing, and excitable structures and motor activity are covered.

This book provides information about plant–environment studies and challenges for plant improvement to achieve food security. Plants face a wide range of environmental challenges, which are expected to become more intense as a result of global climate change. Plant–environment interactions play an important role in the functioning of ecosystems. There are habitats throughout the world that present challenges to crop plants, such as through a lack of water and excessive, or toxic, salts in the soil. Soil properties represent a strong selection pressure for plant diversity and influence the structure of plant communities and participate to the generation and maintenance of biodiversity. Plant communities selected by environment grow by modifying soil physical, chemical, and biological properties, with consequent effects on survival and growth of plants. The complexity of plant–environment interactions has recently been studied by developing a trait-based approach in which responses and effects of plants on environment were quantified and modeled. This fundamental research on plant–environment interaction in ecosystems is essential to transpose knowledges of functional ecology to environmental management. Plants have adapted to an incredible range of environment, and extensive researches on ecological and environmental plant physiology have provided mechanistic understanding of the survival, distribution, productivity, and abundance of plant species across the diverse climates of our planet. Ecophysiological techniques have greatly advanced our understanding of photosynthesis, respiration, plant water relations, and plant responses to abiotic and biotic stresses, from instantaneous to evolutionary timescales. Ecophysiological studies also provide the basis for scaling plant physiological processes from the tissue to the canopy, ecosystem, region, and to a large extent, the entire globe. Given the above, the author proposes to bring forth a comprehensive book, “New Frontiers in Plant-Environment Interactions”, highlighting the various emerging techniques and applications that are currently being used in plant–environment interaction research and its future prospects. The author is sure that this book caters the need of all those who are working or have interest in the above topic.

Whether through loss of habitat or cascading community effects, diseases can shape the very nature of the marine environment. Despite their significant impacts, studies of marine diseases have tended to lag behind their terrestrial equivalents, particularly with regards to their ecological effects. However, in recent decades global research focused on marine disease ecology has expanded at an accelerating rate. This is due in part to increases in disease emergence across many taxa, but can also be attributed to a broader realization that the parasites responsible for disease are themselves important members of marine communities. Understanding their ecological relationships with the environment and their hosts is critical to understanding, conserving, and managing natural and exploited populations, communities, and ecosystems. Courses on marine disease ecology are now starting to emerge and this first textbook in the field will be ideally placed to serve them. Marine Disease Ecology is suitable for graduate students and researchers in the fields of marine disease ecology, aquaculture, fisheries, veterinary science, evolution and conservation. It will also be of relevance and use to a broader interdisciplinary audience of government agencies, NGOs, and marine resource managers.