The first to provide systematically organized information on all three important aspects of artificial receptor design, this book brings together knowledge on an exceptionally hot and multidisciplinary field of research. Strong emphasis is placed on the methodology for discovering artificial receptors, with both definitions for chemosensitivity as well as experimental setups supplied. There follows coverage of numerous classes of artificial receptors, including synthesis, immobilization on surfaces, and quantitative data on properties. The third part of the book focuses on receptor arrays for artificial nose and tongue applications and the whole is rounded off with an outlook and an appendix with all relevant quantitative data on artificial receptors.

Despite achievements in the application of enzymes, antibodies and biological receptors to diagnostics and sensing, the last two decades have also witnessed the emergence of a number of alternative technologies based on synthetic chemistry. This volume shows how synthetic receptors can be designed with characteristics that make them attractive alternatives to biological molecules in the sensory and diagnostics fields, with contributions from leading experts in the area. Subjects covered include synthetic receptors for a range of biomolecules, the use of antimicrobial peptides for the detection of pathogenic microorganisms, the development of molecularly imprinted polymer (MIP) nanoparticles, the in silico design of MIPs and MIP-based sensors, and two chapters examining the development of sensors from an industrial point of view. The particular focus of all chapters is on practical aspects, either in the development process or the applications of the synthesized materials. This book will serve as an important reference work for business leaders and technology experts in the sensors and diagnostics sector.

With contributions by numerous experts

Synthetic receptor molecules, molecules that mimic antibody recognition, are widely used for developing drug leads; drug delivery vehicles; imaging agents; sensing agents; capture agents and separation systems. Synthetic Receptors for Biomolecules covers the most effective synthetic receptors for each major class of biomolecules within the context of specific applications. The book starts with an introduction to the applications of synthetic receptors for biomolecules and their design and synthesis for biomolecule recognition. Dedicated chapters then cover synthetic receptors for the key biomolecules including inorganic cations; small organic and inorganic anions; carbohydrates; nucleosides/nucleotides; oligonucleotides; amino acids and peptides; protein surfaces as well as non-polar and polar lipids; Each chapter follows the same systematic format of (a) chemical structures and physical properties of the biomolecule, (b) biological recognition of the biomolecule, (c) synthetic receptors for the biomolecule, (d) future directions and challenges. Edited by a leader in the field, the book is written in an accessible style for readers new to supramolecular chemistry or for those looking for synthetic receptors.

Biologically inspired approaches for artificial sensing have been extensively applied to different sensory modalities over the last decades and chemical senses have been no exception. The olfactory system, and the gustatory system to a minor extent, has been regarded as a model for the development of new artificial chemical sensing s- tems. One of the main contributions to this field was done by Persaud and Dodd in 1982 when they proposed a system based on an array of broad-selective chemical sensors coupled with a pattern recognition engine. The array aimed at mimicking the sensing strategy followed by the olfactory system where a population of bro- selective olfactory receptor neurons encodes for chemical information as patterns of activity across the neuron population. The pattern recognition engine proposed was not based on bio-inspired but on statistical methods. This influential work gave rise to a new line of research where this paradigm has been used to build chemical sensing instruments applied to a wide range of odor detection problems. More recently, some researchers have proposed to extend the biological inspiration of this system also to the processing of the sensor array signals. This has been mo- vated in part by the increasing body of knowledge available on biological olfaction, which has become in the last decade a focus of attention of the experimental neu- science community.

Biologically inspired approaches for artificial sensing have been extensively applied to different sensory modalities over the last decades and chemical senses have been no exception. The olfactory system, and the gustatory system to a minor extent, has been regarded as a model for the development of new artificial chemical sensing s- tems. One of the main contributions to this field was done by Persaud and Dodd in 1982 when they proposed a system based on an array of broad-selective chemical sensors coupled with a pattern recognition engine. The array aimed at mimicking the sensing strategy followed by the olfactory system where a population of bro- selective olfactory receptor neurons encodes for chemical information as patterns of activity across the neuron population. The pattern recognition engine proposed was not based on bio-inspired but on statistical methods. This influential work gave rise to a new line of research where this paradigm has been used to build chemical sensing instruments applied to a wide range of odor detection problems. More recently, some researchers have proposed to extend the biological inspiration of this system also to the processing of the sensor array signals. This has been mo- vated in part by the increasing body of knowledge available on biological olfaction, which has become in the last decade a focus of attention of the experimental neu- science community.

Key features include: Self-assessment questions and exercises Chapters start with essential principles, then go on to address more advanced topics More than 1300 references to direct the reader to key literature and further reading Highly illustrated with 450 figures, including chemical structures and reactions, functioning principles, constructive details and response characteristics Chemical sensors are self-contained analytical devices that provide real-time information on chemical composition. A chemical sensor integrates two distinct functions: recognition and transduction. Such devices are widely used for a variety of applications, including clinical analysis, environment monitoring and monitoring of industrial processes. This text provides an up-to-date survey of chemical sensor science and technology, with a good balance between classical aspects and contemporary trends. Topics covered include: Structure and properties of recognition materials and reagents, including synthetic, biological and biomimetic materials, microorganisms and whole-cells Physicochemical basis of various transduction methods (electrical, thermal, electrochemical, optical, mechanical and acoustic wave-based) Auxiliary materials used e.g. synthetic and natural polymers, inorganic materials, semiconductors, carbon and metallic materials properties and applications of advanced materials (particularly nanomaterials) in the production of chemical sensors and biosensors Advanced manufacturing methods Sensors obtained by combining particular transduction and recognition methods Mathematical modeling of chemical sensor processes Suitable as a textbook for graduate and final year undergraduate students, and also for researchers in chemistry, biology, physics, physiology, pharmacology and electronic engineering, this bookis valuable to anyone interested in the field of chemical sensors and biosensors.

In a bid to develop synthetic molecules and materials that are capable of mimicking biological recognition and function, intensive research in the fields of synthetic receptor technologies, smart materials, synthetic biology and smart indicators has been under way for the past 20-30 years. The development of synthetic receptors continues to grow rapidly. Novel molecular architectures, with ever improving selective binding properties are constantly being described, and in some cases providing much-needed physical insights into the nature of non-covalent interactions and molecular recognition. Such receptor systems are finding increasingly esoteric applications and this book captures the key developments at the synthetic receptor/biology/detection science interface. The editor has extensive experience in applying smart materials and synthetic receptors to the development of biosensors. Reddy has developed smart, permselective and biocompatible molecularly imprinted polymers and membrane materials for the sensor/sample interface and the advancement of smart materials-based electrochemical, quartz crystal and optical sensors for medical, food and environmental applications. Chapters demonstrate how growing disciplines such as biomimetics, synthetic receptor technologies, pattern recognition and nanotechnology are being used to develop new smart materials for diagnostic sensor and biosensor applications. Postgraduate students and researchers in academia and industry will benefit from this resourceful handbook.

Nanoarmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes, Volume 590 is the latest volume in the Methods in Enzymology series that focuses on nanoarmoring of enzymes and the rational design of polymer-wrapped enzymes. This new volume presents the most updated information on a variety of topics, including specific chapters on Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One, Enzyme Adsorption on Nanoparticle Surfaces Probed by Highly Sensitive Second Harmonic Light Scattering, Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method, and Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts. Users will find this to be an all-encompassing resource on nanoarmoring in enzymes. Focuses on the nanoarmoring of enzymes Covers the rational design of polymer-wrapped enzymes Includes contributions from leading authorities working in enzymology Informs and updates on all the latest developments in the field of enzymology

with contributions by numerous experts