Antisense Technology Part B
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Author |
: |
Publisher |
: Elsevier |
Total Pages |
: 683 |
Release |
: 1999-11-10 |
ISBN-10 |
: 9780080496719 |
ISBN-13 |
: 0080496717 |
Rating |
: 4/5 (19 Downloads) |
Synopsis Antisense Technology, Part B: Applications by :
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedically-oriented research.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
Author |
: |
Publisher |
: |
Total Pages |
: |
Release |
: 2000 |
ISBN-10 |
: OCLC:760565889 |
ISBN-13 |
: |
Rating |
: 4/5 (89 Downloads) |
Synopsis Antisense Technology Part B by :
Author |
: Conrad Lichtenstein |
Publisher |
: IRL Press |
Total Pages |
: 334 |
Release |
: 1997 |
ISBN-10 |
: 0199635838 |
ISBN-13 |
: 9780199635832 |
Rating |
: 4/5 (38 Downloads) |
Synopsis Antisense Technology by : Conrad Lichtenstein
Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation and the use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The application of these technologies are then described, with chapters on antisense techniques in IDictyostelium, plants and the medical uses and benefits of antisense sense technology both in vitro and in vivo. Finally, non-antisense effects of oligonucleotides, "anti-sense rescue", shotgun antisense mutagenesis, and in vivo, in vitro and in situ detection of sense: antisense duplexes by structure-specific anti-RNA antibodies are discussed. The numerous hints and tips for success and advice on trouble shooting, make this authoritative text invaluable to all those researchers who work on gene expression, RNA transcription, or protein function.
Author |
: |
Publisher |
: |
Total Pages |
: |
Release |
: 1993 |
ISBN-10 |
: OCLC:64150672 |
ISBN-13 |
: |
Rating |
: 4/5 (72 Downloads) |
Synopsis Antisense technology by :
Author |
: Stanley T. Crooke |
Publisher |
: CRC Press |
Total Pages |
: 848 |
Release |
: 2007-07-25 |
ISBN-10 |
: 9781439832509 |
ISBN-13 |
: 1439832501 |
Rating |
: 4/5 (09 Downloads) |
Synopsis Antisense Drug Technology by : Stanley T. Crooke
Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research. Interpreting lessons learned from the clinical trials of first generati
Author |
: Cy A. Stein |
Publisher |
: Wiley-Liss |
Total Pages |
: 556 |
Release |
: 1998-03-19 |
ISBN-10 |
: UOM:39015047084978 |
ISBN-13 |
: |
Rating |
: 4/5 (78 Downloads) |
Synopsis Applied Antisense Oligonucleotide Technology by : Cy A. Stein
Applied Antisense Oligonucleotide Technology provides the basic concepts as well as the practical concerns associated with the use of antisense oligonucleotides to modify gene expression. Chapters include oligonucleotide chemistry, DNA triplex formation, delivery mechanisms, pharmocokinetics, toxicity, oligonucleotides.
Author |
: |
Publisher |
: Academic Press |
Total Pages |
: 0 |
Release |
: 1999-10-25 |
ISBN-10 |
: 0121822141 |
ISBN-13 |
: 9780121822149 |
Rating |
: 4/5 (41 Downloads) |
Synopsis Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies by :
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Author |
: |
Publisher |
: Elsevier |
Total Pages |
: 614 |
Release |
: 1999-11-01 |
ISBN-10 |
: 9780080496702 |
ISBN-13 |
: 0080496709 |
Rating |
: 4/5 (02 Downloads) |
Synopsis Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies by :
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Author |
: M. Ian Phillips |
Publisher |
: |
Total Pages |
: |
Release |
: 2000 |
ISBN-10 |
: OCLC:42805668 |
ISBN-13 |
: |
Rating |
: 4/5 (68 Downloads) |
Synopsis Antisense Technology: Applications by : M. Ian Phillips
Author |
: Phillips, I M |
Publisher |
: |
Total Pages |
: 0 |
Release |
: 1999 |
ISBN-10 |
: OCLC:648270298 |
ISBN-13 |
: |
Rating |
: 4/5 (98 Downloads) |
Synopsis Antisense Technology by : Phillips, I M