Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol

The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture.

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions

Allen I. Laskin Biosciences Research Exxon Research and Engineering Company Linden, New Jersey I was contacted in the Fall of 1981 by Professors Martin Dworkin and Palmer Rogers, of the University of Minnesota and asked to participate in the orgnization of the 1983 conference in the series, "Interface Between Biology and Medicine". They and the other members of the advisory committee had the vision to realize that this was a time to depart somewhat from the traditional theme, since one of the major areas of interest in the biological and related sciences these days is that of biotechnology in a broader sense than its impact on medicine alone. In designing the format of the Conference, we considered another factor. There has been a plethora of conferences, symposia, and meetings on biotechnology over the past few years, and the faces and topics have become rather familiar. There has been a strong emphasis on the development of the technology and the "biotechnology industry"; less attention has been paid to the science behind it. One might get the impression from some of these meetings and from the popular press that biotechnology has just recently sprung up, apparently full blown; the very fundamental scientific discoveries and the great body of 1 ALLEN I. LASKIN 2 continuing research that forms that basis for the technology is often obscured.

This book is divided into five sections. The first section deals with the methodology and bioresource generation, techniques related to genetic engineering, and gene transfer to the nuclear genome and chloroplast genome. The new techniques of genome profiling and gene silencing are also presented. The second section of the book covers the classical aspect of plant biotechnology viz. tissue culture and micropropagation. Use of genetic engineering via Agrobacterium and direct transfer of DNA through particle bombardment to develop transformed plants in Artemisia, castor and orchids, and production of recombinant proteins in plant cells have been dealt with in the third section. The fourth section addresses the abiotic and biotic stress tolerance in plants. The basic biology of some of the stress responses, and designing plants for stress tolerance is discussed in this section. The fifth section examines medicinal plants and alkaloid production.

Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.

Automation and Environmental Control in Plant Tissue Culture rigorously explores the new challenges faced by modern plant tissue culture researchers and producers worldwide: issues of cost efficiency, automation, control, and optimization of the in vitro microenvironment. This book achieves a critical balance between the economic, engineering and biological viewpoints, and presents well-balanced, unique, and clearly organized perspectives on current initiatives in the tissue culture arena. Each chapter offers guidelines leading towards an exhaustive, unprecedented level of control over in vitro growth, based on emerging technologies of robotics, machine vision, environmental sensors and regulation, and systems analysis. Unlike other tissue culture books which focus on specific crops and techniques, this book spans the broad range of major tissue culture production systems, and advances evidence on how some underrated aspects of the process actually determine the status of the end product. Key researchers from industry and academia have joined to give up-to-date research evidence and analysis. The collection comprises an essential reference for industrial-scale tissue culture producers, as well as any researcher interested in optimizing in vitro production.

Advances in Gene Technology: Molecular Genetics of Plants and Animals contains the proceedings of the Miami Winter Symposium held in January 1983 in Miami, Florida. The papers explore advances in the molecular genetics of plants and animals and cover a wide range of topics such as genetic manipulation of plants; plant cell cultures, regeneration, and somatic cell fusion; and nitrogen fixation. Practical applications of gene technology with plants are also discussed. Comprised of 84 chapters, this volume begins with an overview of how plants manufacture from carbon dioxide and water all of their substances, paying particular attention to the path of carbon in photosynthesis. The organization of the plant genome is then considered, along with techniques for cell culture, regeneration, and somatic cell fusion; vector systems; and nitrogen fixation. Some chapters focus on gene transfer by protoplast fusion; somatic cell genetic systems in corn; regulation of transcription of the nitrogen fixation operons; and leghemoglobin and nodulin genes of soybean. The final section is devoted to practical applications of gene technology to plants and to technology frontiers in animal biology, in particular embryonic development and vaccines and diagnostic methods for animal diseases. This book should be of value to molecular geneticists.

Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. - Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry - Includes current studies and innovations in biotechnology - Covers commercialization and current perspectives in the field of plant tissue culture techniques

Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.