Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 & 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

An essential reference for any laboratory working in the analytical fluorescence glucose sensing field. The increasing importance of these techniques is typified in one emerging area by developing non-invasive and continuous approaches for physiological glucose monitoring. This volume incorporates analytical fluorescence-based glucose sensing reviews, specialized enough to be attractive to professional researchers, yet appealing to a wider audience of scientists in related disciplines of fluorescence.

Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.

Bioanalytical science and its technological subdomain, biosensors, are ever-evolving subjects, striving for rapid improvement in terms of performance and expanding the target range to meet the vast societal and market demands. The key performance factors for a biosensor that drive the research are selectivity, sensitivity, response time, accuracy, and reproducibility, with additional requirements of its portability and inexpensive nature. These performance factors are largely governed by the materials and techniques being used in these bioanalytical platforms. The selection of materials to meet these requirements is critical, as their interaction or involvement with the biological recognition elements should initiate or improve these performance factors. The technique discussed primarily applies to transducers involved in converting a biochemical signal to optical or electrical signals. Over the years, the emergence of novel materials and techniques has drastically improved the performance of these bioanalytical systems, enabling them to expand their analytical horizon. These advanced materials and techniques are central to modern bioanalytical and biosensor research. Advanced Materials and Techniques for Biosensors and Bioanalytical Applications provides a comprehensive review of the subject, including a knowledge platform for both academics and researchers. Considering biosensors as a central theme to this book, an outline on this subject with background principles has been included, with a scope of extending the utility of the book to coursework in graduate and postgraduate schools. Features: • Basic principles on different classes of biosensors, recent advances and applications • Smart materials for biosensors and other rapid, portable detection devices • Metal nanoparticles and nanocrystals for analytical applications • Carbon-based nanoparticles and quantum dots for sensing applications • Nanozymes as potential catalysts for sensing applications • Bioelectrochemiluminescence and photoelectrochemical-based biosensors • Paper electronics and paper-based biosensors • Microbial biosensors: artificial intelligence, genetic engineering, and synthetic biology • Biofuel cells as a signal transduction platform • FET-based biosensors, including ISFET and BioFET This book serves as a reference for scientific investigators and a textbook for a graduate-level course in biosensors and advanced bioanalytical techniques.

Although nitric oxide (NO) is an important biological signaling molecule, its free-radical electronic configuration makes it a most reactive molecule and the scariest colorless gas causing immense environmental and health hazards. Detection of NO levels in biological samples and in the atmosphere is therefore crucial. In the past few years, extensive efforts have been devoted to developing many active sensors and effective devices for detecting and quantifying atmospheric NO, NO generated in biological samples, and NO exhaled in the human breath. This book provides a concrete summary of recent state-of-the-art small-molecule probes and novel carbon nanomaterials used for chemical, photoluminescent, and electrochemical NO detection. One chapter is especially dedicated to the available devices used for detecting NO in the human breath that indirectly infers to lung inflammation. The authors with expertise in diverse dimensions have attempted to cover almost all areas of NO sensing.

Advances in Aggregation Induced Emission Materials in Biosensing and Imaging for Biomedical Applications - Part A Volume 184, highlights many aspects of AIE materials that can help future investigators, researchers, students and stakeholders perform research with ease. Emitting light is a fascinating photophysical phenomenon, its different forms have brought the attention of various disciplines of natural sciences for centuries. In the modern era of scientific generation, short-lived fluorescence light and its long-lived counterpart phosphorescence light has been employed for several chemo-sensing, bio-sensing, and bioimaging applications. The aggregation induced emission (AIE) phenomenon has appeared as a wand of modern science to convert aggregation-caused quenching (ACQ) materials into AIE active materials for a wide range of biomedical applications including biosensing, bioimaging and localization of molecules for better understanding of molecular mechanisms. This volume covers a wide range of topics which are not currently available in a single volume, including ACQ & AIE concept development; intracellular pH, temperature and viscosity sensing; imaging of cell membrane, lipid droplet, lysosome, and mitochondria; biosensing and Imaging of bacteria; nucleus and nucleic acid imaging. - Offers a basic understanding of AIE principle, mechanism and transformation of ACQ active to AIE active materials - Elucidates nucleus and nucleic acid imaging applications of AIE active small molecules - Describes imaging of cell membrane, lipid droplet, lysosome, and mitochondria of AIE molecules