This publication provides an approach by which conservationists can determine whether or not long-term seed storage is feasible for a particular species, i.e. whether or not that species shows orthodox seed storage behaviour, and provides advice on the implementation of the protocol, examples of ways in which the results from seed storage studies could be misinterpreted due to confounding factors, as well as several alternative approaches for estimating seed storage behaviour prior to carryng out actual investigations with the seeds. In particular, the latter section introduces the concept of a multicriteria approach for estimating seed storage behaviour.

Set includes revised editions of some issues.

"This publication is the result of a joint collaboration between IPGRI, the University of Reading and the Royal Botanic Gardens, Kew..." "The International Plant Genetic Resources Institute (IPGRI) is an autonomous internatinoal scientific organisation operating as part of the Consultative Group on International Agricultural Research (CGIAR). IPGRI's mandate is to advance the conservation and use of plant genetic resources for the benefit of present and future generations."--Page V.

This Manual, based on a regional training course, attempts to clarify most of the concepts and scientific principles for establishing and managing field genebanks. It deals with teh following subjects: the role of field genebank in a complementary conservation strategy, the current status of seed and in vitro and cryopreservation, legal aspects, choice of material and practical aspects of laying out the field plots and planting

Substantial progress has been made in seed science during the past few years, emphasizing its important role in advancing plant biotechnology, agriculture, plant resource management, and conservation. Providing comprehensive coverage of the latest seed science research including germination,. dormancy, development, and desiccation tolerance, this book also details the most advanced methods and practices in seed biology, ecology and technology.

It is a distressing truism that the human race during the last millennium has caused the exponential loss of plant genetic diversity throughout the world. This has had direct and negative economic, political and social consequences for the human race, which at the same time has failed to exploit fully the positive benefits that might result from conserving and exploiting the world's plant genetic resources. However, a strong movement to halt this loss of plant diversity and enhance its utilisation for the benefit of all humanity has been underway since the 1960's (Frankel and Bennett, 1970; Frankel and Hawkes, 1975). This initiative was taken up by the Convention on Biological Diversity (CBD, 1992) that not only expounds the need to conserve biological diversity but links conservation to exploitation and development for the benefit of all. Article 8 of the Convention clearly states the need to develop more effective and efficient guidelines to conserve biological diversity, while Article 9, along with the FAO International Undertaking on Plant Genetic Resources, promotes the adoption of a complementary approach to conservation that incorporates both ex situ and in situ techniques.

Plant genomics and biotechnology have recently made enormous strides, and hold the potential to benefit agriculture, the environment and various other dimensions of the human endeavor. It is no exaggeration to claim that the twenty-first century belongs to biotechnology. Knowledge generation in this field is growing at a frenetic pace, and keeping abreast of the latest advances and calls on us to double our efforts. Volume II of this two-part series addresses cutting-edge aspects of plant genomics and biotechnology. It includes 37 chapters contributed by over 70 researchers, each of which is an expert in his/her own field of research. Biotechnology has helped to solve many conundrums of plant life that had long remained a mystery to mankind. This volume opens with an exhaustive chapter on the role played by thale cress, Arabidopsis thaliana, which is believed to be the Drosophila of the plant kingdom and an invaluable model plant for understanding basic concepts in plant biology. This is followed by chapters on bioremediation, biofuels and biofertilizers through microalgal manipulation, making it a commercializable prospect; discerning finer details of biotic stress with plant-fungal interactions; and the dynamics of abiotic and biotic stresses, which also figure elsewhere in the book. Breeding crop plants for desirable traits has long been an endeavor of biotechnologists. The significance of molecular markers, marker assisted selection and techniques are covered in a dedicated chapter, as are comprehensive reviews on plant molecular biology, DNA fingerprinting techniques, genomic structure and functional genomics. A chapter dedicated to organellar genomes provides extensive information on this important aspect. Elsewhere in the book, the newly emerging area of epigenetics is presented as seen through the lens of biotechnology, showcasing the pivotal role of DNA methylation in effecting permanent and transient changes to the genome. Exclusive chapters deal with bioinformatics and systems biology. Handy tools for practical applications such as somatic embryogenesis and micropropagation are included to provide frontline information to entrepreneurs, as is a chapter on somaclonal variation. Overcoming barriers to sexual incompatibility has also long been a focus of biotechnology, and is addressed in chapters on wide hybridization and hybrid embryo rescue. Another area of accomplishing triploids through endosperm culture is included as a non-conventional breeding strategy. Secondary metabolite production through tissue cultures, which is of importance to industrial scientists, is also covered. Worldwide exchange of plant genetic material is currently an essential topic, as is conserving natural resources in situ. Chapters on in vitro conservation of extant, threatened and other valuable germplasms, gene banking and related issues are included, along with an extensive account of the biotechnology of spices – the low-volume, high-value crops. Metabolic engineering is another emerging field that provides commercial opportunities. As is well known, there is widespread concern over genetically modified crops among the public. GM crops are covered, as are genetic engineering strategies for combating biotic and abiotic stresses where no other solutions are in sight. RNAi- and micro RNA- based strategies for crop improvement have proved to offer novel alternatives to the existing non-conventional techniques, and detailed information on these aspects is also included. The book’s last five chapters are devoted to presenting the various aspects of environmental, marine, desert and rural biotechnology. The state-of-the-art coverage on a wide range of plant genomics and biotechnology topics will be of great interest to post-graduate students and researchers, including the employees of seed and biotechnology companies, and to instructors in the fields of plant genetics, breeding and biotechnology.

Ex situ preservation of germplasm for higher plant species has been accom plished using either seeds or clones, but storage of these under typical condi tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.