A Manual of Paper Chromatography and Paper Electrophoresis provides a comprehensive discussion of the techniques of paper chromatography and paper electrophoresis. The book is organized into two parts. Part I on paper chromatography provides a readily accessible source for some of the many uses and adaptations of paper chromatography. An effort has been made to write a practical manual in which tried and proved procedures, employing relatively simple equipment and available reagents, are summarized. Part II on paper electrophoresis discusses basic principles and methodology. The emphasis throughout has been on the separation of protein mixtures, particularly blood serum. This reflects the fact that it is in this particular application that paper electrophoresis has thus far not been challenged by paper chromatography, whereas many of the smaller molecules can be resolved equally well or better by the thus far more widely employed chromatographic procedures.

Paper chromatography. Theory of paper chromatography. General methods. Amino, Amines, and proteins. Carbohydrates. Aliphatic acids. Steroids, bile acids, and cardiac glycosides. Purines, pyrimidines and related substances. Naturally occurring pigments. Inorganic separations. Paper electrophoresis. General theory. Methods. Continuous electrophoresis.

The Composition, Structure and Reactivity of Proteins

Paper Chromatography and Electrophoresis, Volume I: Electrophoresis in Stabilizing Media covers the general features of electrophoresis in stabilizing media. The book includes a consideration of the factors which determine the rate of movement of the compounds in an electrical field, the factors which must be controlled in order to obtain successful results, as well as the general arrangement and types of equipment used. The text also provides a description of methods for the separation of specific classes of compounds (amines, amino acids, peptides, proteins, nucleic acids, derivatives, and related compounds, carbohydrates, and organic acids and derivatives) normally encountered by chemists. Inorganic chemists, organic chemists, clinical chemists, and biochemists will find the book invaluable.

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

This book intends to be neither a complete survey of the field nor an exhaustive source of references. For these purposes, the use of the extensive compilation "Experimental Immunochemistry" by E. A. KABAT and M. M. MAYER (1962) or the excellent methodological textbook, "Methods in Immunology", by D. H. CAMPBELL, J. S. GARVEY, E. E. CREMER and D. H. SUSSDORF (1963), or the quite comprehensive series "Methods in Immunology and Immunochemistry" by C. A. WILLIAMS and M. W. CHASE (1967) are more suitable. The main purpose of this manual ist to provide students with a simple book which will introduce them to some frequently occurring problems in the three major sections of the immunochemistry of natural products. These are the isolation of the materials, the chemical analysis of the constituents and their structure, and, finally, the assays of the most important biological and immunological activities. In this manual the exercises are simplified and several shortcuts are taken in order to fit them into the framework of a teaching course. The introduction to each exercise gives a brief and elementary explanation of the reaction on which it is based. "Materials and Equipment" lists all tissues or cells, chemicals, glassware, and special equipment which must be available to carry out the exercise, although the very common laboratory tools are usually omitted from the list.