Manual of Spectrofluorometric and Spectrophotometric Derivative Experiments is a superb, self-study manual for technicians and analytical chemists to use for learning how to perform spectrometry and fluorometry experiments. It presents step-by-step procedures for conducting the experiments, and it explains how the instruments work and how to interpret the results. Each experiment in the book includes:

This is a new edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis and phosphorescence. The second edition also introduces new topics such as process, applications, bioprocess monitoring and biotechnology methods, soild surface luminescence and pesticide analysis, providing expanded coverage on chemiluminescence and environmental analysis and updates information on equipment, supplies newer references and more.

Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.

Using this book biochemists can determine how spectrophotometry can contribute to laboratory analyses. Emphasis is placed on the capabilities and limitations of the instrument in use--how to select a machine, how to check if it is working satisfactorily, and what to do if it fails to produce the data expected.

Limnological Analyses, a classic, second, thoroughly updated edition, consists of a series of carefully designed and tested field and laboratory exercises covering the full scope of limnology. It provides the student with a solid foundation in this complex multidisciplinary field of ecology and illustrates modern experimental approaches. Among the topics covered by such exercises are: major physical components of lakes and streams; important mineral nutrients; cycling of organic matter; benthic fauna; primary productivity of phytoplankton; quantitative methods in biota analysis; diurnal changes; experimental manipulation of model ecosystems; effects of sewage outfall and other human activities; whole ecosystem and community analyses. Each exercise is preceded by an introductory section and concludes with questions for the student and a selection of suggested reading. Teachers and students of limnology will value Limnological Analyses for its highly structured, concise presentation. Its research-oriented approach encourages active participation.

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.