The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.

Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.

This intensive manual provides students with valuable information and insights into animal development at the organismal, cellular, and subcellular levels. The book uses both descriptive and investigative approaches that emphasize techniques, key experiments, and data analysis. - Provides a broad introductory view of developmental systems - Teaches both classical embryology and modern experimental approaches - Contains seventeen laboratory exercises, written in step-by-step style - Organized with additional notes to students and preparators - Lists questions and references for each exercise - Special chapters give introductions to the scientific process, use of the microscope, and the writing of scientific papers - Illustrated with detailed line drawings

Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.

This is a lab manual for a college-level human anatomy course. Mastery of anatomy requires a fair amount of memorization and recall skills. The activities in this manual encourage students to engage with new vocabulary in many ways, including grouping key terms, matching terms to structures, recalling definitions, and written exercises. Most of the activities in this manual utilize anatomical models, and several dissections of animal tissues and histological examinations are also included. Each unit includes both pre- and post-lab questions and six lab exercises designed for a classroom where students move from station to station. The vocabulary terms used in each unit are listed at the end of the manual and serve as a checklist for practicals.

The eighth edition of this widely respected volume continues the tradition of introducing laboratory studies of developmental biology with its broad coverage, copious illustrations and detailed descriptions of a wide range of developing stages. Unique in its combination of a detailed atlas with interesting exercises on living embryos, it also contains complete instructions for additional experimental studies that include state-of-the-art research approaches. The eighth edition adds a new chapter on the development of the mouse embryo, many new illustrations, seven new advanced hands-on studies and a glossary.