Benson Fisher thought that a scholarship to Maxfield Academy would be the ticket out of his dead-end life. He was wrong. Now he’s trapped in a school that’s surrounded by a razor-wire fence. A school where video cameras monitor his every move. Where there are no adults. Where the kids have split into groups in order to survive. Where breaking the rules equals death. But when Benson stumbles upon the school’s real secret, he realizes that playing by the rules could spell a fate worse than death, and that escape—his only real hope for survival—may be impossible.

This volume provides practical guidance on a variety of techniques and steps to ensure successful variant calling. Chapters detail methods for variant calling from single-nucleotide variants to structural variants, variant calling in specialized data types such as RNA-seq and UMI-tagged sequencing, alignment-free genotyping and SNP calling, variant detection in single-cell DNA sequencing data, variant annotation, and preanalytical quality control to ensure successful variant calling. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists step-by-step protocol to execute the algorithms, describes the input and output data, and includes tips on troubleshooting and known pitfalls. Authoritative and cutting-edge, Variant Calling: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Clinical DNA Variant Interpretation: Theory and Practice, a new volume in the Translational and Applied Genomics series, covers foundational aspects, modes of analysis, technology, disease and disorder specific case studies, and clinical integration. This book provides a deep theoretical background, as well as applied case studies and methodology, enabling researchers, clinicians and healthcare providers to effectively classify DNA variants associated with disease and patient phenotypes. Practical chapters discuss genomic variant interpretation, terminology and nomenclature, international consensus guidelines, population allele frequency, functional evidence transcripts for RNA, proteins, and enzymes, somatic mutations, somatic profiling, and much more. - Compiles best practices, methods and sound evidence for DNA variant classification in one applied volume - Features chapter contributions from international leaders in the field - Includes practical examples of variant classification for common and rare disorders, and across clinical phenotypes

SysML does not provide explicit built-in language constructs to model variants. Nevertheless SysML is useful to create a model for variants. The VAMOS method presented in the book Variant Modeling with SysML is one option how to model variants with SysML. It uses the profile mechanism of SysML to extend the language with a concept for variant modeling. The concepts are core, variation point, variation, variant, variant constraint, and variant configuration. The book shows how to apply the concepts with standard SysML modeling tool. The book covers * Variant Modeling Concepts * Method for Variant Modeling with SysML (VAMOS) * Variant Stereotypes for SysML * Example: Forest Fire Detection System * Example: Virtual Museum Tour

Perfect for fans of James Dashner's The Maze Runner, Feedback is the heart-stopping sequel to Variant—which Pittacus Lore, author of I Am Number Four, praised as "an intense journey with some of the most shocking twists and turns I've ever read." Benson Fisher escaped from Maxfield Academy's deadly rules and brutal gangs. He thought that the worst was over. But now Benson is trapped in a different kind of prison—a town filled with familiar faces. Classmates from Maxfield who Benson had seen die. Friends he was afraid he had killed. They are all pawns in the school's twisted experiment, held captive and controlled by an unseen force. And while Benson struggles to figure out who, if anyone, can be trusted, he discovers that Maxfield Academy's plans are darker than anything he imagined—and they may be impossible to stop.

A captivating story about finding your soulmates in a world fought with unseen dangers, Variant Lost is an Amazon Top 100 bestseller! One ordinary woman. Four extraordinary men. A power that could ruin them all. Driven by a promise to keep her identity secret, tenacious Evelyn Maynard is all alone in the world. But a scholarship to the exclusive Bradford Hills Institute offers her a safe haven and a fresh start. Or so she thought. Eve is one of the only humans at her new school, brimming with those born with Variant DNA - and coveted superhuman abilities. Bound by shared tragedy, Ethan, Josh, Tyler and Alec - and their unique powers - are revered amongst Variants. But something has always been missing and she may have just arrived with a scholarship and a glint in her enchanting, curious eyes. Together, they could be unimaginably powerful, but being bonded by the Light itself isn't always a blessing, and Eve's dangerous past could curse them all. Because she's not the only one keeping secrets... Can they learn to trust each other in time to save themselves from an insidious danger no one saw coming?

Providers and consumers have to deal with variants of software services, which are alternative instances of a services design, implementation, deployment, or operation. This work develops the service feature modeling language to represent software service variants and a suite of methods to select variants for development or delivery. An evaluation describes the systems implemented to make use of service feature modeling and its application to two real-world use cases.

Chess is a game of wits that calls for strategy, tactics and intuition. The game has a long history spanning several centuries and has been played the world over for it develops strategic thinking and tactical planning skills. Chess is intellectually stimulating and challenging - and offers limitless possibilities at every stage of play. The very nature of the game makes it amenable to systematic study and mastery over time. There are several variants of Chess which offer a level-playing field for amateurs who can think logically. This book is the outcome of the author's curiosity about new Chess variants which can be played on boards with different geometries, different sets of rules with different objectives. This entirely new look at Chess offers: Chess inspired games that reflect situations and events from daily life. Games inspired by popular sports such as squash, volleyball and cricket. Chess games as a motivational tool in education to help students develop interest and liking for their subjects and achieve better academic performance. Chess inspired puzzles. Chess as an outdoor sport and indoor team-game. A. V. Murali's engrossing book highlights the different perspectives of Chess - showcasing its broader applicability, not just for amusement, but for physical and intellectual development as well.