Advanced Protocols for Animal Transgenesis

Advanced Protocols for Animal Transgenesis
Author :
Publisher : Springer Nature
Total Pages : 671
Release :
ISBN-10 : 9783642207921
ISBN-13 : 3642207928
Rating : 4/5 (21 Downloads)

Synopsis Advanced Protocols for Animal Transgenesis by : Shirley Pease

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Transgenesis Techniques

Transgenesis Techniques
Author :
Publisher :
Total Pages : 351
Release :
ISBN-10 : 0896036960
ISBN-13 : 9780896036963
Rating : 4/5 (60 Downloads)

Synopsis Transgenesis Techniques by : Alan R. Clarke

Animal Transgenesis and Cloning

Animal Transgenesis and Cloning
Author :
Publisher : Wiley-Blackwell
Total Pages : 248
Release :
ISBN-10 : STANFORD:36105111818394
ISBN-13 :
Rating : 4/5 (94 Downloads)

Synopsis Animal Transgenesis and Cloning by : Louis-Marie Houdebine

Designed to be a concise, balanced introduction to this dynamic subject, covering key issues and current techniques currently used in animal transgenesis and cloning. Provides the essentials of the subject, from the molecular basis of gene structure and function to therapeutic gene cloning in humans. Social and ethical implications of this important area of research are also considered.

Transgenic Animals

Transgenic Animals
Author :
Publisher : CRC Press
Total Pages : 606
Release :
ISBN-10 : 9057020696
ISBN-13 : 9789057020698
Rating : 4/5 (96 Downloads)

Synopsis Transgenic Animals by : Louis-Marie Houdebine

During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.

Rabbit Biotechnology

Rabbit Biotechnology
Author :
Publisher : Springer Science & Business Media
Total Pages : 135
Release :
ISBN-10 : 9789048122271
ISBN-13 : 9048122279
Rating : 4/5 (71 Downloads)

Synopsis Rabbit Biotechnology by : Louis-Marie Houdebine

Louis-Marie Houdebine and Jianglin Fan The study of biological functions of proteins and their possible roles in the pathogenesis of human diseases requires more and more relevant animal m- els. Although mice including genetically modified mice offer many possibilities, other non-murine species are absolutely required in some circumstances. Rabbit is one of these species, which has been widely used in biomedical studies. This animal is genetically and physiologically closer to humans including cardiov- cular system and metabolism characteristics. Rabbit is thus more appropriate than mice to study some diseases such as atherosclerosis and lipid metabolism. Because of its larger size, surgery manipulation, bleeding, and turn-over studies are much easier performed in rabbits than in mice. Furthermore, transgenic rabbits can be produced using microinjection and other methods such as lentiviral v- tors. Cloning in rabbits has been proved possible, even though still laborious and time-consuming. Hopefully, functional rabbit ES cell lines will be available in the coming years. Gene deletion or knock-out in rabbits will then become possible.

Transgenic Mouse Methods and Protocols

Transgenic Mouse Methods and Protocols
Author :
Publisher : Springer Science & Business Media
Total Pages : 382
Release :
ISBN-10 : 9781592593408
ISBN-13 : 1592593402
Rating : 4/5 (08 Downloads)

Synopsis Transgenic Mouse Methods and Protocols by : Marten H. Hofker

Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.

Genetically Modified Organisms

Genetically Modified Organisms
Author :
Publisher : CRC Press
Total Pages : 124
Release :
ISBN-10 : 9781482280029
ISBN-13 : 1482280027
Rating : 4/5 (29 Downloads)

Synopsis Genetically Modified Organisms by : Yves Tourte

This work reviews the theoretical and historical basis of genetic engineering, particularly in regard to genetically modified plants, and details techniques of creating genetically modified organisms. It describes research programs and results in areas such as agro-food, health, and the environment, and examines practical, legal, and ethical questions posed by society and the responses of scientists, legislators, and industry. B&W photographs of equipments are given.

Safety of Genetically Engineered Foods

Safety of Genetically Engineered Foods
Author :
Publisher : National Academies Press
Total Pages : 254
Release :
ISBN-10 : 9780309166157
ISBN-13 : 0309166152
Rating : 4/5 (57 Downloads)

Synopsis Safety of Genetically Engineered Foods by : National Research Council

Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Insect Transgenesis

Insect Transgenesis
Author :
Publisher : CRC Press
Total Pages : 420
Release :
ISBN-10 : 9781420039399
ISBN-13 : 1420039393
Rating : 4/5 (99 Downloads)

Synopsis Insect Transgenesis by : Alfred M. Handler

Imagine scientists controlling the transmission of certain diseases through the genetic modification of mosquitoes. Eradicating harmful insects without the use of pesticides. Or increasing the fertility of some insects who in turn eat harmful arthropods or even a plant pathogen. Those are just a few of the real-world applications of insect transgen

Nuclear Transfer Protocols

Nuclear Transfer Protocols
Author :
Publisher : Springer Science & Business Media
Total Pages : 352
Release :
ISBN-10 : 9781588292803
ISBN-13 : 1588292800
Rating : 4/5 (03 Downloads)

Synopsis Nuclear Transfer Protocols by : Paul J. Verma

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.