This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.

C. Neal Johnson offers the first comprehensive guide to business as mission (BAM) for practitioners. He provides conceptual foundationas for understanding BAM's unique place in global mission and prerequisites for engaging in it. Then he offers practical resources for how to do BAM, including strategic planning and step-by-step operational implementation.

Through a synthesis of a broader inter-disciplinary literature in the field of disaster studies, organisation theory and management, and an in-depth case study this book provides an analytical framework for organising post-disaster reconstruction programmes which aim to reduce future disaster risks and achieve other objectives. It explores the role of organisational design and management on approaching and achieving the objectives of the reconstruction programme in Iran after the 2003 Bam earthquake. The housing reconstruction programme in Bam was a complex case, offering various learning opportunities to understand organising reconstruction processes especially in urban areas. The case study research explores how the urban housing reconstruction programme system was formed purposefully towards the delivery of the stated objectives and created an innovative housing process throughout the urban area. It identifies consistencies and inconsistencies among the influential organisational attributes of the programme delivery system formation. The system evolved through corrective adjustments (either formally or informally) during its implementation to reflect unfolding consequences of inconsistencies in initial formation and emerging contextual issues in the field.

Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.

A poignant story of a hockey town paralyzed by scandal. Jobs are disappearing and Beartown is slowly dying, so for its citizens, hockey is everything. This is the story not just of hockey, but of a 15-year-old named Maya Andersson, whose father, Peter, the general manager of the hockey club, loves hockey, but loves his family more. Seventeen-year-old Kevin Erdahl is the star of Beartown, with a chance to go professional. One night, after a huge win, Maya goes to a raucous party at Kevin's house and is thrilled at his attention, but things get out of hand, and what takes place changes Beartown forever.

Presenting a theory of the theoryless, a computer scientist provides a model of how effective behavior can be learned even in a world as complex as our own, shedding new light on human nature.

Even though we know full well that most restaurant foods are made using ingredients laden with chemicals and additives, most of us can’t seem to shake the desire for even just a taste. Not to mention that nothing is easier than picking up takeout, hitting the drive-thru, or ordering delivery—but at what cost? Paleo Takeout: Restaurant Favorites Without the Junk delivers much healthier but equally satisfying alternatives, offering delectable recipes that mimic the flavors of our drive-thru and delivery favorites—Paleo style! Russ Crandall teaches you step-by-step how to prepare meals in less than an hour—leaving no sacrifice of taste or time. Our modern lives are hectic: We all face the challenge of creating meals at home that are as quick and flavorful as those from our neighborhood takeout restaurants. It’s hard to beat the convenience of restaurant food, even when we know full well that it’s seldom a healthy choice. In Paleo Takeout: Restaurant Favorites Without the Junk, celebrated author Russ Crandall re-creates everyone’s favorite takeout meals, made in record time using wholesome ingredients, giving you all of the gratification and none of the regret! Inspired by beloved restaurant experiences, Paleo Takeout features more than 200 recipes expertly culled from Chinese, Korean, Japanese, Thai, Vietnamese, Indian, Italian, Mexican, Greek, and American cuisines. Inside, you’ll find everything from Chow Mein to Moo Shu Pork, and Thai Red Curry to Buffalo Wings, all with a focus of “fridge to face” in less than an hour. Also featured is an indispensible meal-planning guide to help you put everything together for a doable, lasting approach to cooking and health. Paleo Takeout: Restaurant Favorites Without the Junk proves that eating right in a way that satisfies even the choosiest of healthy eaters is not only possible but also a lot of fun

If he had been with me everything would have been different... I wasn't with Finn on that August night. But I should've been. It was raining, of course. And he and Sylvie were arguing as he drove down the slick road. No one ever says what they were arguing about. Other people think it's not important. They do not know there is another story. The story that lurks between the facts. What they do not know—the cause of the argument—is crucial. So let me tell you...

Learn how you can tackle everyday leadership challenges regardless of your title, position, or authority with this insightful resource A book about leadership for people who are not in formal or hierarchical leadership positions, Everyday People, Extraordinary Leadership provides readers with a comprehensive and practical approach to addressing leadership challenges, no matter the setting or circumstance. Esteemed scholars and sought-after consultants Jim Kouzes and Barry Posner adapt their trademark The Five Practices of Exemplary Leadership® framework to today’s more horizontal workplace, showing people that leadership is not about where you are in the organization; it’s about how you behave and what you do. Everyday People, Extraordinary Leadership draws on the authors’ deep well of research and practical experience to cover key subjects: The essence of making a difference in any role, setting, or situation The difference between positions of authority and leadership The importance of self-development in leadership development This book is perfectly applicable and accessible for anyone who wants to improve their own leadership potential and who isn’t yet in an official leadership role. Everyday People, Extraordinary Leadership offers authoritative new insights, original case studies and examples, and practical guidance for those individuals who want to make a difference. You supply the will, and this book will supply the way.

NATIONAL BESTSELLER • A curmudgeonly professor journeys to a small town in the far north in this “incredibly fun journey through fae lands and dark magic” (NPR), the start of a heartwarming and enchanting new fantasy series. “A darkly gorgeous fantasy that sparkles with snow and magic.”—Sangu Mandanna, author of The Very Secret Society of Irregular Witches A BEST BOOK OF THE YEAR: The New York Times Book Review, NPR, PopSugar Cambridge professor Emily Wilde is good at many things: She is the foremost expert on the study of faeries. She is a genius scholar and a meticulous researcher who is writing the world’s first encyclopaedia of faerie lore. But Emily Wilde is not good at people. She could never make small talk at a party—or even get invited to one. And she prefers the company of her books, her dog, Shadow, and the Fair Folk to other people. So when she arrives in the hardscrabble village of Hrafnsvik, Emily has no intention of befriending the gruff townsfolk. Nor does she care to spend time with another new arrival: her dashing and insufferably handsome academic rival Wendell Bambleby, who manages to charm the townsfolk, muddle Emily’s research, and utterly confound and frustrate her. But as Emily gets closer and closer to uncovering the secrets of the Hidden Ones—the most elusive of all faeries—lurking in the shadowy forest outside the town, she also finds herself on the trail of another mystery: Who is Wendell Bambleby, and what does he really want? To find the answer, she’ll have to unlock the greatest mystery of all—her own heart. Book One of the Emily Wilde Series