This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

This volume is concerned with the application of fluorescence, phosphorescence and chemiluminescence spectra to the analysis of organic and inorganic compounds. It deals with the use of these spectra to extract information about the structure and function of biological macromolecules. Several of the topics, including excited state optical activity and bioinorganic luminescence spectroscopy, are presented here in an analytical format.

Over the past three decades, luminescence spectroscopy has transcended its origins as a curiosity in the physical laboratory to become a widely used and respected staple of the analytical chemist's instrumentation arsenal. Fluorescence, chemiluminescence, and phosphorescence spectroscopies are now routinely applied to such real analytical problems as the quantitaion, qualitative identification, and structural characterization of organic and inorganic compounds and even of cellular structures. And the list of recent advances in analytical applications of luminescence spectroscopy keeps growing. The earlier volumes of Molecular Luminescence Spectroscopy provided professional chemists with a detailed, exhaustive, and up-to-date look at the applications of fluorescence, phosphorescence, and chemiluminescence spectra to the analysis of organic and inorganic compounds. Presenting topics never available in any analytical text, such as excited state optical activity and bioinorganic luminescence spectroscopy, the volumes represented a significant advance in the chemical literature. Part 3 continues the book's always current and practical examination of the field’s newest innovative turns. In a clear, systematic format, Part 3 discusses such widespread or ascendant laboratory techniques as: photochemically generated fluorophores fluorescent probes luminescence from bile salt aggregates hole-burning spectroscopy laser-excited microspectro-fluorometry near-infrared luminescence spectroscopy Other topics such as the fluorescence and phosphorescence of pharmaceuticals and natural products have never been reviewed as exhaustively before. The chapters on fluorescence detection in chromatography and luminescence immunoassay are the most up-to-date treatments available on these subjects. Invaluable to analytical chemists, instructors, and students, Molecular Luminescence Spectroscopy, Part 3 offers expert guidance on the practical specifics of this multi-faceted technique as well as its farreaching analytical possibilities.

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Concerned with the application of fluorescence, phosphorescence and chemiluminescence spectra to the analysis of organic and inorganic compounds, this book also deals with the use of spectra to extract information about the structure and function of biological macromolecules.

This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Trace Analysis is a highly practical book which deals with the science rather than the paperwork of quality assurance systems. Produced as part of the UK Valid Analytical Measurement (VAM) initiative, it provides the analyst with a systematic approach across the broad spectrum of trace analysis, offering practical advice and guidance on methodology and techniques. The book is structured to take the analyst step-by-step through the stages of any trace analysis. The approach is general, being broken down only into types of analyte. Additional chapters explain the application of groups of techniques to each analyte type. Each section contains references to published material which will allow the analyst to obtain further information on specific topics. Throughout the book, the analyst is reminded of pitfalls which lead to unreliable results. This new book therefore offers invaluable advice to analysts in all areas and at all levels, providing practical 'expert' advice on methodology. It will prove indispensable as a single, comprehensive bench guide for analysts in university, college and industrial laboratories.

A core text on principles, laboratory/field methodologies, and data interpretation for fluorescence applications in aquatic science, for advanced students and researchers.

Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.